Enterovirus particles expel capsid pentamers to enable genome
release

J 2019

Enterovirus particles expel capsid pentamers to enable genome release

BUCHTA, David, Tibor FÜZIK, Dominik HREBÍK, Yevgen LEVDANSKY, Lukáš SUKENÍK et. al.

Základní údaje

Originální název

Enterovirus particles expel capsid pentamers to enable genome release

Autoři

BUCHTA, David (203 Česká republika, domácí), Tibor FÜZIK (703 Slovensko, domácí), Dominik HREBÍK (703 Slovensko, domácí), Yevgen LEVDANSKY (804 Ukrajina, domácí), Lukáš SUKENÍK (203 Česká republika, domácí), Liya MUKHAMEDOVA (643 Rusko, domácí), Jana MORAVCOVÁ (203 Česká republika, domácí), Robert VÁCHA (203 Česká republika, domácí) a Pavel PLEVKA (203 Česká republika, garant, domácí)

Vydání

Nature Communications, London, Nature Publishing Group, 2019, 2041-1723

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10607 Virology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 12.121

Kód RIV

RIV/00216224:14740/19:00107387

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1038/s41467-019-09132-x

UT WoS

000460631100023

Klíčová slova anglicky

MOLECULAR-DYNAMICS; CONFORMATIONAL-CHANGES; FORCE-FIELD; CELL ENTRY; POLIOVIRUS; VIRUS; RNA; MODEL; TRANSITIONS; RECOGNITION

Štítky

CF CRYO, CF PROT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 27. 10. 2024 14:04, Ing. Martina Blahová

Anotace

V originále

Viruses from the genus Enterovirus are important human pathogens. Receptor binding or exposure to acidic pH in endosomes converts enterovirus particles to an activated state that is required for genome release. However, the mechanism of enterovirus uncoating is not well understood. Here, we use cryo-electron microscopy to visualize virions of human echovirus 18 in the process of genome release. We discover that the exit of the RNA from the particle of echovirus 18 results in a loss of one, two, or three adjacent capsid-protein pentamers. The opening in the capsid, which is more than 120 A in diameter, enables the release of the genome without the need to unwind its putative double-stranded RNA segments. We also detect capsids lacking pentamers during genome release from echovirus 30. Thus, our findings uncover a mechanism of enterovirus genome release that could become target for antiviral drugs.

Návaznosti

GX19-25982X, projekt VaV

Název: Analýza replikace enterovirů s využitím elektronové mikroskopie

Investor: Grantová agentura ČR, Structural study of enterovirus replication in situ

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

3041, interní kód MU

Název: Structural studies of human and animal pathogens from the order Picornavirales

Investor: EMBO (European Molecular Biology Organization), Structural studies of human and animal pathogens from the order Picornavirales

335855, interní kód MU

Název: Structural studies of human picornaviruses directed towards development of anti-viral compounds (Akronym: PicoDrugs)

Investor: Evropská unie, Structural studies of human picornaviruses directed towards development of anti-viral compounds, Myšlenky

90043, velká výzkumná infrastruktura

Název: CIISB

Citovat

BUCHTA, David, Tibor FÜZIK, Dominik HREBÍK, Yevgen LEVDANSKY, Lukáš SUKENÍK, Liya MUKHAMEDOVA, Jana MORAVCOVÁ, Robert VÁCHA a Pavel PLEVKA. Enterovirus particles expel capsid pentamers to enable genome release. Nature Communications. London: Nature Publishing Group, 2019, roč. 10, MAR, s. 1-9. ISSN 2041-1723. Dostupné z: https://dx.doi.org/10.1038/s41467-019-09132-x.

@article{1528796,
   author = {Buchta, David and Füzik, Tibor and Hrebík, Dominik and Levdansky, Yevgen and Sukeník, Lukáš and Mukhamedova, Liya and Moravcová, Jana and Vácha, Robert and Plevka, Pavel},
   article_location = {London},
   article_number = {MAR},
   doi = {http://dx.doi.org/10.1038/s41467-019-09132-x},
   keywords = {MOLECULAR-DYNAMICS; CONFORMATIONAL-CHANGES; FORCE-FIELD; CELL ENTRY; POLIOVIRUS; VIRUS; RNA; MODEL; TRANSITIONS; RECOGNITION},
   language = {eng},
   issn = {2041-1723},
   journal = {Nature Communications},
   title = {Enterovirus particles expel capsid pentamers to enable genome release},
   url = {https://www.nature.com/articles/s41467-019-09132-x.pdf},
   volume = {10},
   year = {2019}
}

TY  - JOUR
ID  - 1528796
AU  - Buchta, David - Füzik, Tibor - Hrebík, Dominik - Levdansky, Yevgen - Sukeník, Lukáš - Mukhamedova, Liya - Moravcová, Jana - Vácha, Robert - Plevka, Pavel
PY  - 2019
TI  - Enterovirus particles expel capsid pentamers to enable genome release
JF  - Nature Communications
VL  - 10
IS  - MAR
SP  - 1-9
EP  - 1-9
PB  - Nature Publishing Group
SN  - 20411723
KW  - MOLECULAR-DYNAMICS
KW  - CONFORMATIONAL-CHANGES
KW  - FORCE-FIELD
KW  - CELL ENTRY
KW  - POLIOVIRUS
KW  - VIRUS
KW  - RNA
KW  - MODEL
KW  - TRANSITIONS
KW  - RECOGNITION
UR  - https://www.nature.com/articles/s41467-019-09132-x.pdf
L2  - https://www.nature.com/articles/s41467-019-09132-x.pdf
N2  - Viruses from the genus Enterovirus are important human pathogens. Receptor binding or exposure to acidic pH in endosomes converts enterovirus particles to an activated state that is required for genome release. However, the mechanism of enterovirus uncoating is not well understood. Here, we use cryo-electron microscopy to visualize virions of human echovirus 18 in the process of genome release. We discover that the exit of the RNA from the particle of echovirus 18 results in a loss of one, two, or three adjacent capsid-protein pentamers. The opening in the capsid, which is more than 120 A in diameter, enables the release of the genome without the need to unwind its putative double-stranded RNA segments. We also detect capsids lacking pentamers during genome release from echovirus 30. Thus, our findings uncover a mechanism of enterovirus genome release that could become target for antiviral drugs.
ER  -

BUCHTA, David, Tibor FÜZIK, Dominik HREBÍK, Yevgen LEVDANSKY, Lukáš SUKENÍK, Liya MUKHAMEDOVA, Jana MORAVCOVÁ, Robert VÁCHA a Pavel PLEVKA. Enterovirus particles expel capsid pentamers to enable genome release. \textit{Nature Communications}. London: Nature Publishing Group, 2019, roč.~10, MAR, s.~1-9. ISSN~2041-1723. Dostupné z: https://dx.doi.org/10.1038/s41467-019-09132-x.

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