HARNOŠ, Jakub, M.C.A. CANIZAL, Miroslav JURÁSEK, Jitender KUMAR, Cornelia HOLLER, Alexandra SCHAMBONY, Kateřina HANÁKOVÁ, Ondřej BERNATÍK, Zbyněk ZDRÁHAL, Kristína GÖMÖRYOVÁ, Tomáš GYBEĽ, Tomasz Witold RADASZKIEWICZ, Marek KRAVEC, Lukáš TRANTÍREK, Jan RYNEŠ, Zankruti DAVE, Ana Iris FERNANDEZ-LLAMAZARES, Robert VÁCHA, Konstantinos TRIPSIANES, Carsten HOFFMANN a Vítězslav BRYJA. Dishevelled-3 conformation dynamics analyzed by FRET-based biosensors reveals a key role of casein kinase 1. Nature Communications. London: Nature Publishing Group, 2019, roč. 10, APR 18 2019, s. 1-18. ISSN 2041-1723. Dostupné z: https://dx.doi.org/10.1038/s41467-019-09651-7. |
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@article{1529116, author = {Harnoš, Jakub and Canizal, M.C.A. and Jurásek, Miroslav and Kumar, Jitender and Holler, Cornelia and Schambony, Alexandra and Hanáková, Kateřina and Bernatík, Ondřej and Zdráhal, Zbyněk and Gömöryová, Kristína and Gybeľ, Tomáš and Radaszkiewicz, Tomasz Witold and Kravec, Marek and Trantírek, Lukáš and Ryneš, Jan and Dave, Zankruti and FernandezandLlamazares, Ana Iris and Vácha, Robert and Tripsianes, Konstantinos and Hoffmann, Carsten and Bryja, Vítězslav}, article_location = {London}, article_number = {APR 18 2019}, doi = {http://dx.doi.org/10.1038/s41467-019-09651-7}, keywords = {PARTICLE MESH EWALD; BETA-CATENIN; WNT/BETA-CATENIN; DIX DOMAIN; MOLECULAR-DYNAMICS; STRUCTURAL BASIS; PDZ DOMAIN; DEP DOMAIN; G-PROTEIN; WNT}, language = {eng}, issn = {2041-1723}, journal = {Nature Communications}, title = {Dishevelled-3 conformation dynamics analyzed by FRET-based biosensors reveals a key role of casein kinase 1}, url = {https://www.nature.com/articles/s41467-019-09651-7}, volume = {10}, year = {2019} }
TY - JOUR ID - 1529116 AU - Harnoš, Jakub - Canizal, M.C.A. - Jurásek, Miroslav - Kumar, Jitender - Holler, Cornelia - Schambony, Alexandra - Hanáková, Kateřina - Bernatík, Ondřej - Zdráhal, Zbyněk - Gömöryová, Kristína - Gybeľ, Tomáš - Radaszkiewicz, Tomasz Witold - Kravec, Marek - Trantírek, Lukáš - Ryneš, Jan - Dave, Zankruti - Fernandez-Llamazares, Ana Iris - Vácha, Robert - Tripsianes, Konstantinos - Hoffmann, Carsten - Bryja, Vítězslav PY - 2019 TI - Dishevelled-3 conformation dynamics analyzed by FRET-based biosensors reveals a key role of casein kinase 1 JF - Nature Communications VL - 10 IS - APR 18 2019 SP - 1-18 EP - 1-18 PB - Nature Publishing Group SN - 20411723 KW - PARTICLE MESH EWALD KW - BETA-CATENIN KW - WNT/BETA-CATENIN KW - DIX DOMAIN KW - MOLECULAR-DYNAMICS KW - STRUCTURAL BASIS KW - PDZ DOMAIN KW - DEP DOMAIN KW - G-PROTEIN KW - WNT UR - https://www.nature.com/articles/s41467-019-09651-7 L2 - https://www.nature.com/articles/s41467-019-09651-7 N2 - Dishevelled (DVL) is the key component of the Wnt signaling pathway. Currently, DVL conformational dynamics under native conditions is unknown. To overcome this limitation, we develop the Fluorescein Arsenical Hairpin Binder- (FlAsH-) based FRET in vivo approach to study DVL conformation in living cells. Using this single-cell FRET approach, we demonstrate that (i) Wnt ligands induce open DVL conformation, (ii) DVL variants that are predominantly open, show more even subcellular localization and more efficient membrane recruitment by Frizzled (FZD) and (iii) Casein kinase 1 epsilon (CK1 epsilon) has a key regulatory function in DVL conformational dynamics. In silico modeling and in vitro biophysical methods explain how CK1 epsilon-specific phosphorylation events control DVL conformations via modulation of the PDZ domain and its interaction with DVL C-terminus. In summary, our study describes an experimental tool for DVL conformational sampling in living cells and elucidates the essential regulatory role of CK1 epsilon in DVL conformational dynamics. ER -
HARNOŠ, Jakub, M.C.A. CANIZAL, Miroslav JURÁSEK, Jitender KUMAR, Cornelia HOLLER, Alexandra SCHAMBONY, Kateřina HANÁKOVÁ, Ondřej BERNATÍK, Zbyněk ZDRÁHAL, Kristína GÖMÖRYOVÁ, Tomáš GYBEĽ, Tomasz Witold RADASZKIEWICZ, Marek KRAVEC, Lukáš TRANTÍREK, Jan RYNEŠ, Zankruti DAVE, Ana Iris FERNANDEZ-LLAMAZARES, Robert VÁCHA, Konstantinos TRIPSIANES, Carsten HOFFMANN a Vítězslav BRYJA. Dishevelled-3 conformation dynamics analyzed by FRET-based biosensors reveals a key role of casein kinase 1. \textit{Nature Communications}. London: Nature Publishing Group, 2019, roč.~10, APR 18 2019, s.~1-18. ISSN~2041-1723. Dostupné z: https://dx.doi.org/10.1038/s41467-019-09651-7.
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