Ascites-Derived Extracellular microRNAs as Potential Biomarkers
for Ovarian Cancer

J 2019

Ascites-Derived Extracellular microRNAs as Potential Biomarkers for Ovarian Cancer

ZÁVESKÝ, Luděk, Eva JANDÁKOVÁ, Vít WEINBERGER, Luboš MINÁŘ, Veronika HANZÍKOVÁ et. al.

Základní údaje

Originální název

Ascites-Derived Extracellular microRNAs as Potential Biomarkers for Ovarian Cancer

Autoři

ZÁVESKÝ, Luděk (203 Česká republika, garant), Eva JANDÁKOVÁ (203 Česká republika), Vít WEINBERGER (203 Česká republika, domácí), Luboš MINÁŘ (203 Česká republika, domácí), Veronika HANZÍKOVÁ (203 Česká republika), Daniela DUŠKOVÁ (203 Česká republika), Lenka ZÁVESKÁ DRÁBKOVÁ (203 Česká republika), Iveta SVOBODOVÁ (203 Česká republika) a Aleš HOŘÍNEK (203 Česká republika)

Vydání

REPRODUCTIVE SCIENCES, THOUSAND OAKS, SAGE PUBLICATIONS INC, 2019, 1933-7191

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30214 Obstetrics and gynaecology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.616

Kód RIV

RIV/00216224:14110/19:00109661

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1177/1933719118776808

UT WoS

000461442500009

Klíčová slova anglicky

ovarian cancer; ascites; microRNA; diagnostic markers; prognostic markers

Štítky

14110411, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 16. 5. 2019 12:01, Soňa Böhmová

Anotace

V originále

Ovarian cancer as the most fatal gynecological malignancy is often manifested by excessive fluid accumulation known as ascites or effusion. Ascites-derived microRNAs (miRNAs) may be closely associated with ovarian cancer progression. However, our knowledge of their roles, altered expression, and clinical outcomes remained limited. In this study, large-scale expression profiling of 754 human miRNAs was performed using real-time quantitative polymerase chain reaction and 384-well TaqMan array human miRNA A and B cards to identify differentially expressed miRNAs between extracellular fraction of the ascitic fluid associated with high-grade serous ovarian carcinomas and control plasma. Of the 754 miRNAs, 153 were significantly differentially expressed relative to the controls. Expression of 7 individual miRNAs (miR-200a, miR-200b, miR-200c, miR-141, miR-429, miR-1290, and miR-30a-5p) was further validated in extended sample sets, including serous, endometrioid, and mucinous subtypes. All miR-200 family members and miR-1290 were conspicuously overexpressed, while miR-30a-5p was only weakly overexpressed. The ability of miRNAs expression to discriminate the pathological samples from the controls was strong. Receiver operating characteristic curve analyses found area under the curve (AUC) values of 1.000 for miR-200a, miR-200c, miR-141, miR-429, and miR-1290 and of AUC 0.996 and 0.885 for miR-200b and miR-30a-5p, respectively. Preliminary survival analyses indicated low expression level of miR-200b as significantly related to longer overall survival (hazard ratio [HR]: 0.25, mean survival 44 months), while high expression level was related to poor overall survival (HR: 4.04, mean survival 24 months). Our findings suggested that ascites-derived miRNAs should be further explored and evaluated as potential diagnostic and prognostic biomarkers for ovarian cancer.

Citovat

ZÁVESKÝ, Luděk, Eva JANDÁKOVÁ, Vít WEINBERGER, Luboš MINÁŘ, Veronika HANZÍKOVÁ, Daniela DUŠKOVÁ, Lenka ZÁVESKÁ DRÁBKOVÁ, Iveta SVOBODOVÁ a Aleš HOŘÍNEK. Ascites-Derived Extracellular microRNAs as Potential Biomarkers for Ovarian Cancer. REPRODUCTIVE SCIENCES. THOUSAND OAKS: SAGE PUBLICATIONS INC, 2019, roč. 26, č. 4, s. 510-522. ISSN 1933-7191. Dostupné z: https://dx.doi.org/10.1177/1933719118776808.

@article{1531300,
   author = {Záveský, Luděk and Jandáková, Eva and Weinberger, Vít and Minář, Luboš and Hanzíková, Veronika and Dušková, Daniela and Záveská Drábková, Lenka and Svobodová, Iveta and Hořínek, Aleš},
   article_location = {THOUSAND OAKS},
   article_number = {4},
   doi = {http://dx.doi.org/10.1177/1933719118776808},
   keywords = {ovarian cancer; ascites; microRNA; diagnostic markers; prognostic markers},
   language = {eng},
   issn = {1933-7191},
   journal = {REPRODUCTIVE SCIENCES},
   title = {Ascites-Derived Extracellular microRNAs as Potential Biomarkers for Ovarian Cancer},
   url = {http://dx.doi.org/10.1177/1933719118776808},
   volume = {26},
   year = {2019}
}

TY  - JOUR
ID  - 1531300
AU  - Záveský, Luděk - Jandáková, Eva - Weinberger, Vít - Minář, Luboš - Hanzíková, Veronika - Dušková, Daniela - Záveská Drábková, Lenka - Svobodová, Iveta - Hořínek, Aleš
PY  - 2019
TI  - Ascites-Derived Extracellular microRNAs as Potential Biomarkers for Ovarian Cancer
JF  - REPRODUCTIVE SCIENCES
VL  - 26
IS  - 4
SP  - 510-522
EP  - 510-522
PB  - SAGE PUBLICATIONS INC
SN  - 19337191
KW  - ovarian cancer
KW  - ascites
KW  - microRNA
KW  - diagnostic markers
KW  - prognostic markers
UR  - http://dx.doi.org/10.1177/1933719118776808
L2  - http://dx.doi.org/10.1177/1933719118776808
N2  - Ovarian cancer as the most fatal gynecological malignancy is often manifested by excessive fluid accumulation known as ascites or effusion. Ascites-derived microRNAs (miRNAs) may be closely associated with ovarian cancer progression. However, our knowledge of their roles, altered expression, and clinical outcomes remained limited. In this study, large-scale expression profiling of 754 human miRNAs was performed using real-time quantitative polymerase chain reaction and 384-well TaqMan array human miRNA A and B cards to identify differentially expressed miRNAs between extracellular fraction of the ascitic fluid associated with high-grade serous ovarian carcinomas and control plasma. Of the 754 miRNAs, 153 were significantly differentially expressed relative to the controls. Expression of 7 individual miRNAs (miR-200a, miR-200b, miR-200c, miR-141, miR-429, miR-1290, and miR-30a-5p) was further validated in extended sample sets, including serous, endometrioid, and mucinous subtypes. All miR-200 family members and miR-1290 were conspicuously overexpressed, while miR-30a-5p was only weakly overexpressed. The ability of miRNAs expression to discriminate the pathological samples from the controls was strong. Receiver operating characteristic curve analyses found area under the curve (AUC) values of 1.000 for miR-200a, miR-200c, miR-141, miR-429, and miR-1290 and of AUC 0.996 and 0.885 for miR-200b and miR-30a-5p, respectively. Preliminary survival analyses indicated low expression level of miR-200b as significantly related to longer overall survival (hazard ratio [HR]: 0.25, mean survival 44 months), while high expression level was related to poor overall survival (HR: 4.04, mean survival 24 months). Our findings suggested that ascites-derived miRNAs should be further explored and evaluated as potential diagnostic and prognostic biomarkers for ovarian cancer.
ER  -

ZÁVESKÝ, Luděk, Eva JANDÁKOVÁ, Vít WEINBERGER, Luboš MINÁŘ, Veronika HANZÍKOVÁ, Daniela DUŠKOVÁ, Lenka ZÁVESKÁ DRÁBKOVÁ, Iveta SVOBODOVÁ a Aleš HOŘÍNEK. Ascites-Derived Extracellular microRNAs as Potential Biomarkers for Ovarian Cancer. \textit{REPRODUCTIVE SCIENCES}. THOUSAND OAKS: SAGE PUBLICATIONS INC, 2019, roč.~26, č.~4, s.~510-522. ISSN~1933-7191. Dostupné z: https://dx.doi.org/10.1177/1933719118776808.

Podrobný výpis o publikaci