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@article{1534736,
author = {Šimečková, Šárka and Kahounova, Zuzana and Fedr, Radek and Remšík, Ján and Slabáková, Eva and Suchankova, Tereza and Procházková, Jiřina and Bouchal, Jan and Kharaishvili, Gvantsa and Král, Milan and Beneš, Petr and Souček, Karel},
article_location = {LONDON},
article_number = {APR},
doi = {http://dx.doi.org/10.1038/s41598-019-42131-y},
keywords = {DEPENDENT KINASE INHIBITOR; STEM-CELLS; DOCETAXEL RESISTANCE; MOLECULAR SIGNATURE; TRANSITION; P27; EMT; ACQUISITION; PROGRESSION; P27(KIP1)},
language = {eng},
issn = {2045-2322},
journal = {Scientific reports},
title = {High Skp2 expression is associated with a mesenchymal phenotype and increased tumorigenic potential of prostate cancer cells},
url = {https://www.nature.com/articles/s41598-019-42131-y},
volume = {9},
year = {2019}
}
TY - JOUR
ID - 1534736
AU - Šimečková, Šárka - Kahounova, Zuzana - Fedr, Radek - Remšík, Ján - Slabáková, Eva - Suchankova, Tereza - Procházková, Jiřina - Bouchal, Jan - Kharaishvili, Gvantsa - Král, Milan - Beneš, Petr - Souček, Karel
PY - 2019
TI - High Skp2 expression is associated with a mesenchymal phenotype and increased tumorigenic potential of prostate cancer cells
JF - Scientific reports
VL - 9
IS - APR
SP - 1-10
EP - 1-10
PB - NATURE PUBLISHING GROUP
SN - 20452322
KW - DEPENDENT KINASE INHIBITOR
KW - STEM-CELLS
KW - DOCETAXEL RESISTANCE
KW - MOLECULAR SIGNATURE
KW - TRANSITION
KW - P27
KW - EMT
KW - ACQUISITION
KW - PROGRESSION
KW - P27(KIP1)
UR - https://www.nature.com/articles/s41598-019-42131-y
L2 - https://www.nature.com/articles/s41598-019-42131-y
N2 - Skp2 is a crucial component of SCFskP2 E3 ubiquitin ligase and is often overexpressed in various types of cancer, including prostate cancer (PCa). The epithelial-to-mesenchymal transition (EMT) is involved in PCa progression. The acquisition of a mesenchymal phenotype that results in a cancer stem cell (CSC) phenotype in PCa was described. Therefore, we aimed to investigate the expression and localization of Skp2 in clinical samples from patients with PCa, the association of Skp2 with EMT status, and the role of Skp2 in prostate CSC. We found that nuclear expression of Skp2 was increased in patients with PCa compared to those with benign hyperplasia, and correlated with high Gleason score in PCa patients. Increased Skp2 expression was observed in PCa cell lines with mesenchymal and CSC-like phenotype compared to their epithelial counterparts. Conversely, the CSC-like phenotype was diminished in cells in which SKP2 expression was silenced. Furthermore, we observed that Skp2 downregulation led to the decrease in subpopulation of CD44(+)CD24(-) cancer stem-like cells. Finally, we showed that high expression levels of both CD24 and CD44 were associated with favorable recurrence-free survival for PCa patients. This study uncovered the Skp2-mediated CSC-like phenotype with oncogenic functions in PCa.
ER -
ŠIMEČKOVÁ, Šárka, Zuzana KAHOUNOVA, Radek FEDR, Ján REMŠÍK, Eva SLABÁKOVÁ, Tereza SUCHANKOVA, Jiřina PROCHÁZKOVÁ, Jan BOUCHAL, Gvantsa KHARAISHVILI, Milan KRÁL, Petr BENEŠ and Karel SOUČEK. High Skp2 expression is associated with a mesenchymal phenotype and increased tumorigenic potential of prostate cancer cells. \textit{Scientific reports}. LONDON: NATURE PUBLISHING GROUP, 2019, vol.~9, APR, p.~1-10. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/s41598-019-42131-y.
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