The effect of shock freezing on physiological properties and
consequent growth of Antarctic filamentous (Stigeoclonium sp.)
and coccal alga (Diplosphaera chodatii) on agar plates

J 2019

The effect of shock freezing on physiological properties and consequent growth of Antarctic filamentous (Stigeoclonium sp.) and coccal alga (Diplosphaera chodatii) on agar plates

OREKHOVA, Alla; Miloš BARTÁK; Aleyna OZKAR a Josef ELSTER

Základní údaje

Originální název

The effect of shock freezing on physiological properties and consequent growth of Antarctic filamentous (Stigeoclonium sp.) and coccal alga (Diplosphaera chodatii) on agar plates

Název česky

Vliv šokového zmražení na fyziologické vlastnosti a následný růst antarktické vláknité (Stigeoclonium sp.) a kokální řasy (Diplosphaera chodatii)v agarové kultuře

Autoři

OREKHOVA, Alla; Miloš BARTÁK; Aleyna OZKAR a Josef ELSTER

Vydání

Czech Polar Reports, Masarykova univerzita, 2019, 1805-0689

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Full Text

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/19:00109872

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.5817/CPR2019-1-4

EID Scopus

2-s2.0-85079839504

Klíčová slova česky

Antarktida; stres chladem; kryoresistence; řasové kultury; biotechnologie

Klíčová slova anglicky

Antarctica; cold stress; cryoresistance; algal cultures; biotechnologies

Štítky

rivok

Příznaky

Recenzováno

Změněno: 18. 3. 2020 15:06, Mgr. Marie Novosadová Šípková, DiS.

Anotace

ORIG CZ

V originále

In this study, we investigated the effects of shock freezing on physiological properties and consequent growth of in the Antarctic alga Stigeoclonium sp. and comparative coccal alga Diplosphaera chodatii on agar plates. Culture of algae grown in liquid medium were used to study subzero temperatures on the species resistance to shock freezing. Then, microalgae were frozen in liquid nitrogen and inoculated on BBM agar after thawing. Physiological status of algae was evaluated by chlorophyll fluorescence parameters during 28 days. The results showed that interspecific differences existed in their tolerance to shock freezing, as well as their consequent growth rate on agars. Direct effects of freezing in liquid nitrogen was demonstrated in chlorophyll fluorescence parameters recorded immediately after thawing the samples (in liquid medium). In spite of the fact that majority of cells was destroyed by shock freezing, the potential of photochemical processes in PS II (FV/FM) remained constant in D. chodatii. It may indicate high resistance of the species to freezing/thawing cycles and a capability of the surviving cells, core chlorophylls in PS II respectively, to perform photosynthetic processes related to PS II. Contrastingly, Stigeoclonium sp. showed a shock freezing-dependent decrease in FV/FM. When shock-frozen, thawed and inoculated on agar plates, the culture of D. chodatii, and Stigeoclonium sp. showed cultivation time-dependent increase in chlorophyll fluorescence parameters (FV/FM, FS).

Česky

V této studii jsme zkoumali účinky šokového zmrazení na fyziologické vlastnosti a následný růst v antarktické řase Stigeoclonium sp. a srovnávací kokální řase Diplosphaera chodatii na agarových kulturách.

Návaznosti

EF16_013/0001708, projekt VaV

Název: ECOPOLARIS - Změny ve struktuře a funkci součástí terestrických polárních ekosystémů (CzechPolar2)

LM2015078, projekt VaV

Název: Česká polární výzkumná infrastruktura (Akronym: CzechPolar2)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Polar Research Infrastructure

Přiložené soubory

Orekhova_web.pdf

Požádat o autorskou verzi souboru

Citovat

OREKHOVA, Alla; Miloš BARTÁK; Aleyna OZKAR a Josef ELSTER. The effect of shock freezing on physiological properties and consequent growth of Antarctic filamentous (Stigeoclonium sp.) and coccal alga (Diplosphaera chodatii) on agar plates. Czech Polar Reports. Masarykova univerzita, 2019, roč. 9, č. 1, s. 37-48. ISSN 1805-0689. Dostupné z: https://doi.org/10.5817/CPR2019-1-4.

@article{1538259,
   author = {Orekhova, Alla and Barták, Miloš and Ozkar, Aleyna and Elster, Josef},
   article_number = {1},
   doi = {https://doi.org/10.5817/CPR2019-1-4},
   keywords = {Antarctica; cold stress; cryoresistance; algal cultures; biotechnologies},
   language = {eng},
   issn = {1805-0689},
   journal = {Czech Polar Reports},
   title = {The effect of shock freezing on physiological properties and consequent growth of Antarctic filamentous (Stigeoclonium sp.) and coccal alga (Diplosphaera chodatii) on agar plates},
   url = {http://www.sci.muni.cz/CPR/17cislo/Orekhova_web.pdf},
   volume = {9},
   year = {2019}
}

TY  - JOUR
ID  - 1538259
AU  - Orekhova, Alla - Barták, Miloš - Ozkar, Aleyna - Elster, Josef
PY  - 2019
TI  - The effect of shock freezing on physiological properties and consequent growth of Antarctic filamentous (Stigeoclonium sp.) and coccal alga (Diplosphaera chodatii) on agar plates
JF  - Czech Polar Reports
VL  - 9
IS  - 1
SP  - 37-48
EP  - 37-48
PB  - Masarykova univerzita
SN  - 18050689
KW  - Antarctica
KW  - cold stress
KW  - cryoresistance
KW  - algal cultures
KW  - biotechnologies
UR  - http://www.sci.muni.cz/CPR/17cislo/Orekhova_web.pdf
L2  - http://www.sci.muni.cz/CPR/17cislo/Orekhova_web.pdf
N2  - In this study, we investigated the effects of shock freezing on physiological properties and consequent growth of in the Antarctic alga Stigeoclonium sp. and comparative coccal alga Diplosphaera chodatii on agar plates. Culture of algae grown in liquid medium were used to study subzero temperatures on the species resistance to shock freezing. Then, microalgae were frozen in liquid nitrogen and inoculated on BBM agar after thawing. Physiological status of algae was evaluated by chlorophyll fluorescence parameters during 28 days. The results showed that interspecific differences existed in their tolerance to shock freezing, as well as their consequent growth rate on agars. Direct effects of freezing in liquid nitrogen was demonstrated in chlorophyll fluorescence parameters recorded immediately after thawing the samples (in liquid medium). In spite of the fact that majority of cells was destroyed by shock freezing, the potential of photochemical processes in PS II (FV/FM) remained constant in D. chodatii. It may indicate high resistance of the species to freezing/thawing cycles and a capability of the surviving cells, core chlorophylls in PS II respectively, to perform photosynthetic processes related to PS II. Contrastingly, Stigeoclonium sp. showed a shock freezing-dependent decrease in FV/FM. When shock-frozen, thawed and inoculated on agar plates, the culture of D. chodatii, and Stigeoclonium sp. showed cultivation time-dependent increase in chlorophyll fluorescence parameters (FV/FM, FS).
ER  -

OREKHOVA, Alla; Miloš BARTÁK; Aleyna OZKAR a Josef ELSTER. The effect of shock freezing on physiological properties and consequent growth of Antarctic filamentous (Stigeoclonium sp.) and coccal alga (Diplosphaera chodatii) on agar plates. \textit{Czech Polar Reports}. Masarykova univerzita, 2019, roč.~9, č.~1, s.~37-48. ISSN~1805-0689. Dostupné z: https://doi.org/10.5817/CPR2019-1-4.

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