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@article{1538259,
author = {Orekhova, Alla and Barták, Miloš and Ozkar, Aleyna and Elster, Josef},
article_number = {1},
doi = {http://dx.doi.org/10.5817/CPR2019-1-4},
keywords = {Antarctica; cold stress; cryoresistance; algal cultures; biotechnologies},
language = {eng},
issn = {1805-0689},
journal = {Czech Polar Reports},
title = {The effect of shock freezing on physiological properties and consequent growth of Antarctic filamentous (Stigeoclonium sp.) and coccal alga (Diplosphaera chodatii) on agar plates},
url = {http://www.sci.muni.cz/CPR/17cislo/Orekhova_web.pdf},
volume = {9},
year = {2019}
}
TY - JOUR
ID - 1538259
AU - Orekhova, Alla - Barták, Miloš - Ozkar, Aleyna - Elster, Josef
PY - 2019
TI - The effect of shock freezing on physiological properties and consequent growth of Antarctic filamentous (Stigeoclonium sp.) and coccal alga (Diplosphaera chodatii) on agar plates
JF - Czech Polar Reports
VL - 9
IS - 1
SP - 37-48
EP - 37-48
PB - Masarykova univerzita
SN - 18050689
KW - Antarctica
KW - cold stress
KW - cryoresistance
KW - algal cultures
KW - biotechnologies
UR - http://www.sci.muni.cz/CPR/17cislo/Orekhova_web.pdf
L2 - http://www.sci.muni.cz/CPR/17cislo/Orekhova_web.pdf
N2 - In this study, we investigated the effects of shock freezing on physiological properties and consequent growth of in the Antarctic alga Stigeoclonium sp. and comparative coccal alga Diplosphaera chodatii on agar plates. Culture of algae grown in liquid medium were used to study subzero temperatures on the species resistance to shock freezing. Then, microalgae were frozen in liquid nitrogen and inoculated on BBM agar after thawing. Physiological status of algae was evaluated by chlorophyll fluorescence parameters during 28 days. The results showed that interspecific differences existed in their tolerance to shock freezing, as well as their consequent growth rate on agars. Direct effects of freezing in liquid nitrogen was demonstrated in chlorophyll fluorescence parameters recorded immediately after thawing the samples (in liquid medium). In spite of the fact that majority of cells was destroyed by shock freezing, the potential of photochemical processes in PS II (FV/FM) remained constant in D. chodatii. It may indicate high resistance of the species to freezing/thawing cycles and a capability of the surviving cells, core chlorophylls in PS II respectively, to perform photosynthetic processes related to PS II. Contrastingly, Stigeoclonium sp. showed a shock freezing-dependent decrease in FV/FM. When shock-frozen, thawed and inoculated on agar plates, the culture of D. chodatii, and Stigeoclonium sp. showed cultivation time-dependent increase in chlorophyll fluorescence parameters (FV/FM, FS).
ER -
OREKHOVA, Alla, Miloš BARTÁK, Aleyna OZKAR a Josef ELSTER. The effect of shock freezing on physiological properties and consequent growth of Antarctic filamentous (Stigeoclonium sp.) and coccal alga (Diplosphaera chodatii) on agar plates. \textit{Czech Polar Reports}. Masarykova univerzita, 2019, roč.~9, č.~1, s.~37-48. ISSN~1805-0689. Dostupné z: https://dx.doi.org/10.5817/CPR2019-1-4.
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