Oral administration of BDNF and/or GDNF normalizes serum BDNF
level in the olfactory bulbectomized rats: A proof of concept
study

J 2019

Oral administration of BDNF and/or GDNF normalizes serum BDNF level in the olfactory bulbectomized rats: A proof of concept study

KUČERA, Jan, Jana RUDÁ, Filip ZLÁMAL, Daniela KURUCZOVÁ, Zuzana BABINSKÁ et. al.

Základní údaje

Originální název

Oral administration of BDNF and/or GDNF normalizes serum BDNF level in the olfactory bulbectomized rats: A proof of concept study

Autoři

KUČERA, Jan (203 Česká republika, domácí), Jana RUDÁ (203 Česká republika, domácí), Filip ZLÁMAL (203 Česká republika, domácí), Daniela KURUCZOVÁ (703 Slovensko, domácí), Zuzana BABINSKÁ (703 Slovensko, domácí), Josef TOMANDL (203 Česká republika, domácí), Marie TOMANDLOVÁ (203 Česká republika, domácí) a Julie DOBROVOLNÁ (203 Česká republika, garant, domácí)

Vydání

Pharmacological Reports, KRAKOW, POLISH ACAD SCIENCES INST PHARMACOLOGY, 2019, 1734-1140

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30104 Pharmacology and pharmacy

Stát vydavatele

Polsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.754

Kód RIV

RIV/00216224:14110/19:00109876

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1016/j.pharep.2019.03.005

UT WoS

000475847600014

Klíčová slova anglicky

BDNF; GDNF; Neurotrophins; Olfactory bulbectomy; Rats

Štítky

14110512, 14110516, 14110518, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 4. 3. 2020 15:21, Mgr. Tereza Miškechová

Anotace

V originále

Background: Neurotrophins, especially brain-derived neurotrophic factor (BDNF) have gained significant therapeutic interest particularly in neurologic and psychiatric disorders and they have been found in human breast milk of mothers who suffered from adverse outcomes in pregnancy. This study tested the hypothesis that oral administration of BDNF/GDNF (glial cell line-derived neurotrophic factor) can exert a biological effect in a rat model of severe neuropathology induced by olfactory bulbectomy (OBX), which exhibits dysregulation of BDNF signaling and impaired blood-brain barrier. Methods: Adult male albino Sprague-Dawley rats underwent the OBX surgery and separate groups of OBX and sham-operated controls received one oral dose of vehicle, BDNF (0.005 mg/kg), GDNF (0.03 mg/kg) or their combination. One week after neurotrophin dosing the rats were sacrificed and BDNF level was assessed by ELISA in the blood serum and cerebrospinal fluid. Results: A significant decrease of serum BDNF level was found in the OBX model. This alteration was normalized by all types of treatment BDNF, GDNF, or their combination. No influence of sham surgery or treatment was observed in the control rats. BDNF levels in cerebrospinal fluid were below detection limit. Conclusion: This study indicates that oral administration of neurotrophins is able to exert a biological effect in the OBX model. There is a number of potential mechanisms, which remain to be elucidated.

Návaznosti

EF15_003/0000469, projekt VaV

Název: Cetocoen Plus

MUNI/A/1550/2018, interní kód MU

Název: Farmakologický výzkum v oblasti farmakokinetiky, neuropsychofarmakologie a onkologie

Investor: Masarykova univerzita, Farmakologický výzkum v oblasti farmakokinetiky, neuropsychofarmakologie a onkologie, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

MUNI/A/1553/2018, interní kód MU

Název: Genetické, environmentální a tkáňové charakteristiky vybraných patologických stavů a nemocí (Akronym: genetika; stres; biomateriály; tkáňové kultury)

Investor: Masarykova univerzita, Genetické, environmentální a tkáňové charakteristiky vybraných patologických stavů a nemocí, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

ROZV/24/LF/2018, interní kód MU

Název: LF - Příspěvek na IP 2108

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, LF - Příspěvek na IP 2108, Interní rozvojové projekty

Citovat

KUČERA, Jan, Jana RUDÁ, Filip ZLÁMAL, Daniela KURUCZOVÁ, Zuzana BABINSKÁ, Josef TOMANDL, Marie TOMANDLOVÁ a Julie DOBROVOLNÁ. Oral administration of BDNF and/or GDNF normalizes serum BDNF level in the olfactory bulbectomized rats: A proof of concept study. Pharmacological Reports. KRAKOW: POLISH ACAD SCIENCES INST PHARMACOLOGY, 2019, roč. 71, č. 4, s. 669-675. ISSN 1734-1140. Dostupné z: https://dx.doi.org/10.1016/j.pharep.2019.03.005.

@article{1538496,
   author = {Kučera, Jan and Rudá, Jana and Zlámal, Filip and Kuruczová, Daniela and Babinská, Zuzana and Tomandl, Josef and Tomandlová, Marie and Dobrovolná, Julie},
   article_location = {KRAKOW},
   article_number = {4},
   doi = {http://dx.doi.org/10.1016/j.pharep.2019.03.005},
   keywords = {BDNF; GDNF; Neurotrophins; Olfactory bulbectomy; Rats},
   language = {eng},
   issn = {1734-1140},
   journal = {Pharmacological Reports},
   title = {Oral administration of BDNF and/or GDNF normalizes serum BDNF level in the olfactory bulbectomized rats: A proof of concept study},
   url = {https://www.sciencedirect.com/science/article/pii/S1734114018305516?via%3Dihub},
   volume = {71},
   year = {2019}
}

TY  - JOUR
ID  - 1538496
AU  - Kučera, Jan - Rudá, Jana - Zlámal, Filip - Kuruczová, Daniela - Babinská, Zuzana - Tomandl, Josef - Tomandlová, Marie - Dobrovolná, Julie
PY  - 2019
TI  - Oral administration of BDNF and/or GDNF normalizes serum BDNF level in the olfactory bulbectomized rats: A proof of concept study
JF  - Pharmacological Reports
VL  - 71
IS  - 4
SP  - 669-675
EP  - 669-675
PB  - POLISH ACAD SCIENCES INST PHARMACOLOGY
SN  - 17341140
KW  - BDNF
KW  - GDNF
KW  - Neurotrophins
KW  - Olfactory bulbectomy
KW  - Rats
UR  - https://www.sciencedirect.com/science/article/pii/S1734114018305516?via%3Dihub
L2  - https://www.sciencedirect.com/science/article/pii/S1734114018305516?via%3Dihub
N2  - Background: Neurotrophins, especially brain-derived neurotrophic factor (BDNF) have gained significant therapeutic interest particularly in neurologic and psychiatric disorders and they have been found in human breast milk of mothers who suffered from adverse outcomes in pregnancy. This study tested the hypothesis that oral administration of BDNF/GDNF (glial cell line-derived neurotrophic factor) can exert a biological effect in a rat model of severe neuropathology induced by olfactory bulbectomy (OBX), which exhibits dysregulation of BDNF signaling and impaired blood-brain barrier. Methods: Adult male albino Sprague-Dawley rats underwent the OBX surgery and separate groups of OBX and sham-operated controls received one oral dose of vehicle, BDNF (0.005 mg/kg), GDNF (0.03 mg/kg) or their combination. One week after neurotrophin dosing the rats were sacrificed and BDNF level was assessed by ELISA in the blood serum and cerebrospinal fluid. Results: A significant decrease of serum BDNF level was found in the OBX model. This alteration was normalized by all types of treatment BDNF, GDNF, or their combination. No influence of sham surgery or treatment was observed in the control rats. BDNF levels in cerebrospinal fluid were below detection limit. Conclusion: This study indicates that oral administration of neurotrophins is able to exert a biological effect in the OBX model. There is a number of potential mechanisms, which remain to be elucidated.
ER  -

KUČERA, Jan, Jana RUDÁ, Filip ZLÁMAL, Daniela KURUCZOVÁ, Zuzana BABINSKÁ, Josef TOMANDL, Marie TOMANDLOVÁ a Julie DOBROVOLNÁ. Oral administration of BDNF and/or GDNF normalizes serum BDNF level in the olfactory bulbectomized rats: A proof of concept study. \textit{Pharmacological Reports}. KRAKOW: POLISH ACAD SCIENCES INST PHARMACOLOGY, 2019, roč.~71, č.~4, s.~669-675. ISSN~1734-1140. Dostupné z: https://dx.doi.org/10.1016/j.pharep.2019.03.005.

Podrobný výpis o publikaci