The Methodology for Endoluminal Irreversible Electroporation in
Porcine Models

J 2019

The Methodology for Endoluminal Irreversible Electroporation in Porcine Models

ANDRAŠINA, Tomáš, Tomáš GROLICH, Michal CRHA, Ladislav STEHLIK, Tomáš ROHAN et. al.

Základní údaje

Originální název

The Methodology for Endoluminal Irreversible Electroporation in Porcine Models

Autoři

ANDRAŠINA, Tomáš (703 Slovensko, garant, domácí), Tomáš GROLICH (203 Česká republika, domácí), Michal CRHA (203 Česká republika), Ladislav STEHLIK (203 Česká republika), Tomáš ROHAN (203 Česká republika, domácí), Červinka DALIBOR (203 Česká republika), Veronika NOVOTNÁ (203 Česká republika), Vladan BERNARD (203 Česká republika, domácí), Iva SVOBODOVÁ (203 Česká republika, domácí) a Vlastimil VÁLEK (203 Česká republika, domácí)

Vydání

Acta Veterinaria Brno, Brno, Veterinární a farmaceutická univerzita Brno, 2019, 0001-7213

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30224 Radiology, nuclear medicine and medical imaging

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 0.566

Kód RIV

RIV/00216224:14110/19:00108487

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.2754/avb201988020201

UT WoS

000509755300008

Klíčová slova anglicky

biliary ablation; magnetic resonance imaging; intraductal ablation; experimental study

Štítky

14110112, 14110216, 14110223, 14110511, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 5. 2020 13:01, Mgr. Tereza Miškechová

Anotace

V originále

The aim of work was to describe methodology of surgical technique for endoluminal irreversible electroporation in the biliary tract performed within the perihilar region in porcine models. Endoluminal irreversible electroporation of the common bile duct was performed on 8 porcine models using an endoluminal device inserted during laparotomy. The endoluminal device consisted of 3 electrodes 1 cm in length, attached at 120 degrees around the balloon catheter. The procedure was conducted with following parameters: number of pulses 90, voltage of 1500 V between each couple of electrodes. Cross sectional imaging and histopathological assessment were employed for evaluations of ablation zone. Models were sacrificed in 24 hours and 96 hours after ablation. The treatment was successful in all porcine models. All animals survived the defined study period. Peri-ablation oedema within the hepatoduodenal ligament and adjacent liver tissue could be measured on post-procedural MRI or CT. Perforation in site of ablation developed in one model. Histopathological examination showed heavy regressive changes of ablated tissue. The elastic membranes of adjacent portal vein were preserved in all models. This novel endoluminal modality used within the perihilar region on porcine models is a feasible and well predictable procedure according to our experience. Further studies should explore the optimal protocol of catheter-based ablation to limit complications.

Návaznosti

NV15-32484A, projekt VaV

Název: Využití nových biotechnologií v prevenci a léčbě stenóz žlučových cest

ROZV/24/LF/2018, interní kód MU

Název: LF - Příspěvek na IP 2108

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, LF - Příspěvek na IP 2108, Interní rozvojové projekty

Citovat

ANDRAŠINA, Tomáš, Tomáš GROLICH, Michal CRHA, Ladislav STEHLIK, Tomáš ROHAN, Červinka DALIBOR, Veronika NOVOTNÁ, Vladan BERNARD, Iva SVOBODOVÁ a Vlastimil VÁLEK. The Methodology for Endoluminal Irreversible Electroporation in Porcine Models. Acta Veterinaria Brno. Brno: Veterinární a farmaceutická univerzita Brno, 2019, roč. 88, č. 2, s. 201-211. ISSN 0001-7213. Dostupné z: https://dx.doi.org/10.2754/avb201988020201.

@article{1541718,
   author = {Andrašina, Tomáš and Grolich, Tomáš and Crha, Michal and Stehlik, Ladislav and Rohan, Tomáš and Dalibor, Červinka and Novotná, Veronika and Bernard, Vladan and Svobodová, Iva and Válek, Vlastimil},
   article_location = {Brno},
   article_number = {2},
   doi = {http://dx.doi.org/10.2754/avb201988020201},
   keywords = {biliary ablation; magnetic resonance imaging; intraductal ablation; experimental study},
   language = {eng},
   issn = {0001-7213},
   journal = {Acta Veterinaria Brno},
   title = {The Methodology for Endoluminal Irreversible Electroporation in Porcine Models},
   url = {http://dx.doi.org/10.2754/avb201988020201},
   volume = {88},
   year = {2019}
}

TY  - JOUR
ID  - 1541718
AU  - Andrašina, Tomáš - Grolich, Tomáš - Crha, Michal - Stehlik, Ladislav - Rohan, Tomáš - Dalibor, Červinka - Novotná, Veronika - Bernard, Vladan - Svobodová, Iva - Válek, Vlastimil
PY  - 2019
TI  - The Methodology for Endoluminal Irreversible Electroporation in Porcine Models
JF  - Acta Veterinaria Brno
VL  - 88
IS  - 2
SP  - 201-211
EP  - 201-211
PB  - Veterinární a farmaceutická univerzita Brno
SN  - 00017213
KW  - biliary ablation
KW  - magnetic resonance imaging
KW  - intraductal ablation
KW  - experimental study
UR  - http://dx.doi.org/10.2754/avb201988020201
L2  - http://dx.doi.org/10.2754/avb201988020201
N2  - The aim of work was to describe methodology of surgical technique for endoluminal irreversible electroporation in the biliary tract performed within the perihilar region in porcine models. Endoluminal irreversible electroporation of the common bile duct was performed on 8 porcine models using an endoluminal device inserted during laparotomy. The endoluminal device consisted of 3 electrodes 1 cm in length, attached at 120 degrees around the balloon catheter. The procedure was conducted with following parameters: number of pulses 90, voltage of 1500 V between each couple of electrodes. Cross sectional imaging and histopathological assessment were employed for evaluations of ablation zone. Models were sacrificed in 24 hours and 96 hours after ablation. The treatment was successful in all porcine models. All animals survived the defined study period. Peri-ablation oedema within the hepatoduodenal ligament and adjacent liver tissue could be measured on post-procedural MRI or CT. Perforation in site of ablation developed in one model. Histopathological examination showed heavy regressive changes of ablated tissue. The elastic membranes of adjacent portal vein were preserved in all models. This novel endoluminal modality used within the perihilar region on porcine models is a feasible and well predictable procedure according to our experience. Further studies should explore the optimal protocol of catheter-based ablation to limit complications.
ER  -

ANDRAŠINA, Tomáš, Tomáš GROLICH, Michal CRHA, Ladislav STEHLIK, Tomáš ROHAN, Červinka DALIBOR, Veronika NOVOTNÁ, Vladan BERNARD, Iva SVOBODOVÁ a Vlastimil VÁLEK. The Methodology for Endoluminal Irreversible Electroporation in Porcine Models. \textit{Acta Veterinaria Brno}. Brno: Veterinární a farmaceutická univerzita Brno, 2019, roč.~88, č.~2, s.~201-211. ISSN~0001-7213. Dostupné z: https://dx.doi.org/10.2754/avb201988020201.

Podrobný výpis o publikaci