a 2019

Ampérometrický biosenzor pre rýchle stanovenie albumínu v moči

ŠIŠKOVÁ, Martina, Matěj PASTUCHA, Marta FARKOVÁ, Přemysl LUBAL, Zdeněk FARKA et. al.

Basic information

Original name

Ampérometrický biosenzor pre rýchle stanovenie albumínu v moči

Name (in English)

Amperometric Biosensor for Rapid Determination of Albumin in the Urine

Authors

Edition

22. ročník celostátní soutěže o nejlepší studentskou vědeckou práci v oboru analytická chemie Cena Karla Štulíka 2019, 2019

Other information

Language

Slovak

Type of outcome

Konferenční abstrakt

Field of Study

10406 Analytical chemistry

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Organization unit

Central European Institute of Technology

ISSN

Keywords in English

amperometry; human serum albumin; biosensor
Změněno: 27/7/2019 16:11, doc. Mgr. Zdeněk Farka, Ph.D.

Abstract

In English

This work presents the development of an amperometric biosensor for the rapid determination of clinically relevant concentrations of human serum albumin in the urine, whose increased concentration indicates nephropathy. This simple method is characterized by a short analysis time, low cost of determination, possibility of miniaturization, automatization and point of care analysis. The assay was performed by using various methods of immobilization the antibody to screen-printed electrodes by means of mixed and flow-through configuration. By immobilizing the antibody via cysteamine and glutaraldehyde in a mixed analysis, a detection limit of 10 ng ml–1 was reached, allowing the determination of human serum albumin in the urine.

Links

LQ1601, research and development project
Name: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR
MUNI/A/1237/2016, interní kód MU
Name: Vývoj metod a instrumentace pro analýzu biologicky významných látek 2017
Investor: Masaryk University, Category A