Detailed Information on Publication Record
2019
Ampérometrický biosenzor pre rýchle stanovenie albumínu v moči
ŠIŠKOVÁ, Martina, Matěj PASTUCHA, Marta FARKOVÁ, Přemysl LUBAL, Zdeněk FARKA et. al.Basic information
Original name
Ampérometrický biosenzor pre rýchle stanovenie albumínu v moči
Name (in English)
Amperometric Biosensor for Rapid Determination of Albumin in the Urine
Authors
Edition
22. ročník celostátní soutěže o nejlepší studentskou vědeckou práci v oboru analytická chemie Cena Karla Štulíka 2019, 2019
Other information
Language
Slovak
Type of outcome
Konferenční abstrakt
Field of Study
10406 Analytical chemistry
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Organization unit
Central European Institute of Technology
ISSN
Keywords in English
amperometry; human serum albumin; biosensor
Změněno: 27/7/2019 16:11, doc. Mgr. Zdeněk Farka, Ph.D.
Abstract
In English
This work presents the development of an amperometric biosensor for the rapid determination of clinically relevant concentrations of human serum albumin in the urine, whose increased concentration indicates nephropathy. This simple method is characterized by a short analysis time, low cost of determination, possibility of miniaturization, automatization and point of care analysis. The assay was performed by using various methods of immobilization the antibody to screen-printed electrodes by means of mixed and flow-through configuration. By immobilizing the antibody via cysteamine and glutaraldehyde in a mixed analysis, a detection limit of 10 ng ml–1 was reached, allowing the determination of human serum albumin in the urine.
Links
LQ1601, research and development project |
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MUNI/A/1237/2016, interní kód MU |
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