Immunohistochemical analysis of retraction pocket pars tensa of
tympanic membrane in children

J 2019

Immunohistochemical analysis of retraction pocket pars tensa of tympanic membrane in children

URÍK, Milan, Pavel HURNIK, Dušan ŽIAK, Josef MACHAČ, Ivo ŠLAPÁK et. al.

Základní údaje

Originální název

Immunohistochemical analysis of retraction pocket pars tensa of tympanic membrane in children

Autoři

URÍK, Milan (703 Slovensko, garant, domácí), Pavel HURNIK (203 Česká republika), Dušan ŽIAK (203 Česká republika), Josef MACHAČ (203 Česká republika, domácí), Ivo ŠLAPÁK (203 Česká republika, domácí), Oldřich MOTYKA (203 Česká republika) a Petr JABANDŽIEV (203 Česká republika, domácí)

Vydání

International journal of pediatric otorhinolaryngology, Amsterdam, Elsevier, 2019, 0165-5876

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30206 Otorhinolaryngology

Stát vydavatele

Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.241

Kód RIV

RIV/00216224:14110/19:00110123

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1016/j.ijporl.2019.04.008

UT WoS

000471738900019

Klíčová slova anglicky

Retraction pocket; Cholesteatoma; Immunohistochemistry; Chidren; Pars tensa

Štítky

14110317, 14110319, rivok

Změněno: 10. 7. 2019 11:25, Soňa Böhmová

Anotace

V originále

Aims: Immunohistochemical analysis of retraction pocket pars tensa of tympanic membrane in children. Identification of signs typical for cholesteatoma and support of retraction theory of cholesteatoma. Study design: a prospective study analysing 31 surgically removed retraction pockets. Department: University Hospital, Children's Medical Centre Methods: Retraction pockets processed by a standard process for immunohistochemical analysis. The observed findings were specified using antibodies CD45 LCA (leukocyte common antigen), CD31 (platelet endothelial cell adhesion molecule), D2-40 (marker of lymphatic endothelium), MMP9 (marker of degradation of connective tissue extracellular matrix) and Ki67 (cellular marker of proliferation). Results: All observed parameters except for MMP9 had a significantly higher incidence in retraction pocket stage III compared to stage II according to Charachon. Conclusion: We described immunohistochemical signs of retraction pocket pars tensa of tympanic membrane in children resulting in cholesteatoma. All the observed signs occur in the structure of matrix and perimatrix of cholesteatoma. A significantly higher incidence of all observed parameters except from MMP9 was proved in retraction pocket stage III, unlike in stage II. This observation proves the fact that retraction pocket is a progressive disease and is a procholesteatoma stage.

Návaznosti

MUNI/A/1002/2018, interní kód MU

Název: Ztráta sluchu v dětském věku - příčiny, diagnostika, možnosti rehabilitace III

Investor: Masarykova univerzita, Ztráta sluchu v dětském věku - příčiny, diagnostika, možnosti rehabilitace III, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

URÍK, Milan, Pavel HURNIK, Dušan ŽIAK, Josef MACHAČ, Ivo ŠLAPÁK, Oldřich MOTYKA a Petr JABANDŽIEV. Immunohistochemical analysis of retraction pocket pars tensa of tympanic membrane in children. International journal of pediatric otorhinolaryngology. Amsterdam: Elsevier, 2019, roč. 122, JUL 2019, s. 111-116. ISSN 0165-5876. Dostupné z: https://dx.doi.org/10.1016/j.ijporl.2019.04.008.

@article{1545096,
   author = {Urík, Milan and Hurnik, Pavel and Žiak, Dušan and Machač, Josef and Šlapák, Ivo and Motyka, Oldřich and Jabandžiev, Petr},
   article_location = {Amsterdam},
   article_number = {JUL 2019},
   doi = {http://dx.doi.org/10.1016/j.ijporl.2019.04.008},
   keywords = {Retraction pocket; Cholesteatoma; Immunohistochemistry; Chidren; Pars tensa},
   language = {eng},
   issn = {0165-5876},
   journal = {International journal of pediatric otorhinolaryngology},
   title = {Immunohistochemical analysis of retraction pocket pars tensa of tympanic membrane in children},
   url = {http://dx.doi.org/10.1016/j.ijporl.2019.04.008},
   volume = {122},
   year = {2019}
}

TY  - JOUR
ID  - 1545096
AU  - Urík, Milan - Hurnik, Pavel - Žiak, Dušan - Machač, Josef - Šlapák, Ivo - Motyka, Oldřich - Jabandžiev, Petr
PY  - 2019
TI  - Immunohistochemical analysis of retraction pocket pars tensa of tympanic membrane in children
JF  - International journal of pediatric otorhinolaryngology
VL  - 122
IS  - JUL 2019
SP  - 111-116
EP  - 111-116
PB  - Elsevier
SN  - 01655876
KW  - Retraction pocket
KW  - Cholesteatoma
KW  - Immunohistochemistry
KW  - Chidren
KW  - Pars tensa
UR  - http://dx.doi.org/10.1016/j.ijporl.2019.04.008
L2  - http://dx.doi.org/10.1016/j.ijporl.2019.04.008
N2  - Aims: Immunohistochemical analysis of retraction pocket pars tensa of tympanic membrane in children. Identification of signs typical for cholesteatoma and support of retraction theory of cholesteatoma. Study design: a prospective study analysing 31 surgically removed retraction pockets. Department: University Hospital, Children's Medical Centre Methods: Retraction pockets processed by a standard process for immunohistochemical analysis. The observed findings were specified using antibodies CD45 LCA (leukocyte common antigen), CD31 (platelet endothelial cell adhesion molecule), D2-40 (marker of lymphatic endothelium), MMP9 (marker of degradation of connective tissue extracellular matrix) and Ki67 (cellular marker of proliferation). Results: All observed parameters except for MMP9 had a significantly higher incidence in retraction pocket stage III compared to stage II according to Charachon. Conclusion: We described immunohistochemical signs of retraction pocket pars tensa of tympanic membrane in children resulting in cholesteatoma. All the observed signs occur in the structure of matrix and perimatrix of cholesteatoma. A significantly higher incidence of all observed parameters except from MMP9 was proved in retraction pocket stage III, unlike in stage II. This observation proves the fact that retraction pocket is a progressive disease and is a procholesteatoma stage.
ER  -

URÍK, Milan, Pavel HURNIK, Dušan ŽIAK, Josef MACHAČ, Ivo ŠLAPÁK, Oldřich MOTYKA a Petr JABANDŽIEV. Immunohistochemical analysis of retraction pocket pars tensa of tympanic membrane in children. \textit{International journal of pediatric otorhinolaryngology}. Amsterdam: Elsevier, 2019, roč.~122, JUL 2019, s.~111-116. ISSN~0165-5876. Dostupné z: https://dx.doi.org/10.1016/j.ijporl.2019.04.008.

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