Detection and identification of fungi in bronchoalveolar lavage
fluid from immunocompromised patients using panfungal PCR

J 2019

Detection and identification of fungi in bronchoalveolar lavage fluid from immunocompromised patients using panfungal PCR

ŘÍČNÁ, Dita, Martina LENGEROVÁ, Matěj BEZDÍČEK, Iva KOCMANOVÁ, Lubos DRGONA et. al.

Základní údaje

Originální název

Detection and identification of fungi in bronchoalveolar lavage fluid from immunocompromised patients using panfungal PCR

Autoři

ŘÍČNÁ, Dita (203 Česká republika, domácí), Martina LENGEROVÁ (203 Česká republika, domácí), Matěj BEZDÍČEK (203 Česká republika, domácí), Iva KOCMANOVÁ (203 Česká republika), Lubos DRGONA (703 Slovensko), Barbora WEINBERGEROVÁ (203 Česká republika, domácí), Jiří MAYER (203 Česká republika, domácí) a Zdeněk RÁČIL (203 Česká republika, domácí)

Vydání

Folia microbiologica, Dordrecht, Springer, 2019, 0015-5632

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30204 Oncology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.730

Kód RIV

RIV/00216224:14110/19:00108353

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1007/s12223-018-00669-w

UT WoS

000468847600014

Klíčová slova anglicky

fungal pneumonia

Štítky

14110212, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 12. 7. 2019 16:43, Soňa Böhmová

Anotace

V originále

Rapid diagnostics of fungal pneumonia and initiation of appropriate therapy are still challenging. In this study, we used two panfungal assays to test bronchoalveolar lavage fluid (BALF) samples to prove their ability to confirm invasive fungal disease diagnosis and identify causative agents. Two methods targeting different fungal rDNA regions were used, and the obtained PCR products were sequenced directly or after cloning. In total, 106 BALF samples from 104 patients were tested. After sequencing, we obtained 578 sequences. Four hundred thirty-seven sequences were excluded from further analysis due to duplication (n=335) or similarity with sequences detected in the extraction control sample (n=102); 141 unique sequences were analyzed. Altogether, 23/141 (16%) of the fungi detected belonged to pathogenic species, and 63/141 (45%) were identified as various yeasts; a variety of environmental or very rare fungal human pathogens represented 29/141 (21%) of the total and 26/141 (18%) were described as uncultured fungus. Panfungal PCR detected fungal species that would be missed by specific methods in only one case (probable cryptococcosis). Panfungal PCR followed by sequencing has limited use for testing BALF samples due to frequent commensal or environmental fungal species pickup.

Návaznosti

MUNI/A/1105/2018, interní kód MU

Název: Nové přístupy ve výzkumu, diagnostice a terapii hematologických malignit VI (Akronym: VýDiTeHeMA VI)

Investor: Masarykova univerzita, Nové přístupy ve výzkumu, diagnostice a terapii hematologických malignit VI, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

TE02000058, projekt VaV

Název: Centrum kompetence pro molekulární diagnostiku a personalizovanou medicínu (Akronym: MOLDIMED)

Investor: Technologická agentura ČR, Centrum kompetence pro molekulární diagnostiku a personalizovanou medicínu

Citovat

ŘÍČNÁ, Dita, Martina LENGEROVÁ, Matěj BEZDÍČEK, Iva KOCMANOVÁ, Lubos DRGONA, Barbora WEINBERGEROVÁ, Jiří MAYER a Zdeněk RÁČIL. Detection and identification of fungi in bronchoalveolar lavage fluid from immunocompromised patients using panfungal PCR. Folia microbiologica. Dordrecht: Springer, 2019, roč. 64, č. 3, s. 421-428. ISSN 0015-5632. Dostupné z: https://dx.doi.org/10.1007/s12223-018-00669-w.

@article{1545498,
   author = {Říčná, Dita and Lengerová, Martina and Bezdíček, Matěj and Kocmanová, Iva and Drgona, Lubos and Weinbergerová, Barbora and Mayer, Jiří and Ráčil, Zdeněk},
   article_location = {Dordrecht},
   article_number = {3},
   doi = {http://dx.doi.org/10.1007/s12223-018-00669-w},
   keywords = {fungal pneumonia},
   language = {eng},
   issn = {0015-5632},
   journal = {Folia microbiologica},
   title = {Detection and identification of fungi in bronchoalveolar lavage fluid from immunocompromised patients using panfungal PCR},
   url = {http://dx.doi.org/10.1007/s12223-018-00669-w},
   volume = {64},
   year = {2019}
}

TY  - JOUR
ID  - 1545498
AU  - Říčná, Dita - Lengerová, Martina - Bezdíček, Matěj - Kocmanová, Iva - Drgona, Lubos - Weinbergerová, Barbora - Mayer, Jiří - Ráčil, Zdeněk
PY  - 2019
TI  - Detection and identification of fungi in bronchoalveolar lavage fluid from immunocompromised patients using panfungal PCR
JF  - Folia microbiologica
VL  - 64
IS  - 3
SP  - 421-428
EP  - 421-428
PB  - Springer
SN  - 00155632
KW  - fungal pneumonia
UR  - http://dx.doi.org/10.1007/s12223-018-00669-w
L2  - http://dx.doi.org/10.1007/s12223-018-00669-w
N2  - Rapid diagnostics of fungal pneumonia and initiation of appropriate therapy are still challenging. In this study, we used two panfungal assays to test bronchoalveolar lavage fluid (BALF) samples to prove their ability to confirm invasive fungal disease diagnosis and identify causative agents. Two methods targeting different fungal rDNA regions were used, and the obtained PCR products were sequenced directly or after cloning. In total, 106 BALF samples from 104 patients were tested. After sequencing, we obtained 578 sequences. Four hundred thirty-seven sequences were excluded from further analysis due to duplication (n=335) or similarity with sequences detected in the extraction control sample (n=102); 141 unique sequences were analyzed. Altogether, 23/141 (16%) of the fungi detected belonged to pathogenic species, and 63/141 (45%) were identified as various yeasts; a variety of environmental or very rare fungal human pathogens represented 29/141 (21%) of the total and 26/141 (18%) were described as uncultured fungus. Panfungal PCR detected fungal species that would be missed by specific methods in only one case (probable cryptococcosis). Panfungal PCR followed by sequencing has limited use for testing BALF samples due to frequent commensal or environmental fungal species pickup.
ER  -

ŘÍČNÁ, Dita, Martina LENGEROVÁ, Matěj BEZDÍČEK, Iva KOCMANOVÁ, Lubos DRGONA, Barbora WEINBERGEROVÁ, Jiří MAYER a Zdeněk RÁČIL. Detection and identification of fungi in bronchoalveolar lavage fluid from immunocompromised patients using panfungal PCR. \textit{Folia microbiologica}. Dordrecht: Springer, 2019, roč.~64, č.~3, s.~421-428. ISSN~0015-5632. Dostupné z: https://dx.doi.org/10.1007/s12223-018-00669-w.

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