J 2019

Current vascular allograft procurement, cryopreservation and transplantation techniques in the Czech Republic

ŠPAČEK, Miroslav, Pavel MĚŘIČKA, Libor JANOUŠEK, Petr ŠTÁDLER, Miloš ADAMEC et. al.

Základní údaje

Originální název

Current vascular allograft procurement, cryopreservation and transplantation techniques in the Czech Republic

Autoři

ŠPAČEK, Miroslav (203 Česká republika, garant), Pavel MĚŘIČKA (203 Česká republika), Libor JANOUŠEK (203 Česká republika), Petr ŠTÁDLER (203 Česká republika), Miloš ADAMEC (203 Česká republika), Robert VLACHOVSKÝ (203 Česká republika, domácí), Igor GUŇKA (203 Česká republika), Pavel NAVRÁTIL (203 Česká republika), Filip THIEME (203 Česká republika), Rudolf ŠPUNDA (203 Česká republika), Jan BURKERT (203 Česká republika), Robert STAFFA (203 Česká republika, domácí), Petr NĚMEC (203 Česká republika) a Jaroslav LINDNER (203 Česká republika)

Vydání

ADVANCES IN CLINICAL AND EXPERIMENTAL MEDICINE, WROCLAW, WROCLAW MEDICAL UNIV, 2019, 1899-5276

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30212 Surgery

Stát vydavatele

Polsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Impakt faktor

Impact factor: 1.514

Kód RIV

RIV/00216224:14110/19:00110189

Organizační jednotka

Lékařská fakulta

UT WoS

000466338100012

Klíčová slova anglicky

tissue banking; cryopreserved vascular allograft transplantation; operative procedures; graft procurement; cryopreservation

Štítky

Příznaky

Mezinárodní význam, Recenzováno
Změněno: 16. 7. 2019 15:22, Soňa Böhmová

Anotace

V originále

Background. Vascular allotransplantations are performed worldwide in selected patients suffering from vascular prosthesis infection or critical limb ischemia. Either fresh or cryopreserved vascular allograft may be used. Objectives. In various points, we address several aspects (allograft procurement, cryopreservation and transplantation technique) of the program of vascular allotransplantations in the Czech Republic. Materials and methods. Vascular grafts retrieval has been done within multiorgan harvests using no-touch technique. Very short time of cold ischemia is achieved due to close cooperation with Tissue Establishment where the following processing of cryopreservation is performed. Meeting all necessary quality criteria is a prerequisity for releasing grafts for clinical application. Standardized thawing protocol and surgical handling aims to minimize microfractures before implantation. Results. Based on experimental and clinical work, the first validation of cryopreserved arterial and venous grafts for clinical use was performed between 2011 and 2013 in the Czech Republic. The developement of storage of vascular tissue in banks was stimulated in 2000-2010 by the issue of EU directives and national harmonized norms, aimed at assurance of high quality and safety of cells and tissues used for transplantations in humans. Conclusions. There are several crucial moments affecting final quality, including graft retrieval within a multiorgan harvest, short ischemic time, cryopreservation, and thawing technique used. The recommended surgical handling during implantation may also affect results and graft-related complications.