2019
Prediction of neuroblastoma cell response to treatment with natural or synthetic retinoids using selected protein biomarkers
DOBROTKOVÁ, Viera, Petr CHLAPEK, Marta JEŽOVÁ, Kateřina ADÁMKOVÁ, Pavel MAZÁNEK et. al.Základní údaje
Originální název
Prediction of neuroblastoma cell response to treatment with natural or synthetic retinoids using selected protein biomarkers
Autoři
DOBROTKOVÁ, Viera (703 Slovensko, domácí), Petr CHLAPEK (203 Česká republika, domácí), Marta JEŽOVÁ (203 Česká republika, domácí), Kateřina ADÁMKOVÁ (203 Česká republika, domácí), Pavel MAZÁNEK (203 Česká republika, domácí), Jaroslav ŠTĚRBA (203 Česká republika, domácí) a Renata VESELSKÁ (203 Česká republika, garant, domácí)
Vydání
PLOS ONE, San Francisco, Public Library of Science, 2019, 1932-6203
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
30204 Oncology
Stát vydavatele
Spojené státy
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Impakt faktor
Impact factor: 2.740
Kód RIV
RIV/00216224:14310/19:00108499
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000471234500064
Klíčová slova anglicky
Neuroblastoma; induced differentiation; retinoids; retinoic acid; bexarotene; fenretinide; biomarkers
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 11. 5. 2020 09:53, Mgr. Marie Šípková, DiS.
Anotace
V originále
Although the administration of retinoids represents an important part of treatment for children suffering from high-risk neuroblastomas, approximately 50% of these patients do not respond to this therapy or develop resistance to retinoids during treatment. Our study focused on the comparative analysis of the expression of five genes and corresponding proteins (DDX39A, HMGA1, HMGA2, HOXC9 and PBX1) that have recently been discussed as possible predictive biomarkers of clinical response to retinoid differentiation therapy. Expression of these five candidate biomarkers was evaluated at both the mRNA and protein level in the same subset of 8 neuroblastoma cell lines after treatment with natural or synthetic retinoids. We found that the cell lines that were HMGA2-positive and/or HOXC9-negative have a reduced sensitivity to retinoids. Furthermore, the experiments revealed that the retinoid-sensitive cell lines showed a uniform pattern of change after treatment with both natural and sensitive retinoids: increased DDX39A and decreased PBX1 protein levels. Our results showed that in NBL cells, these putative protein biomarkers are associated with sensitivity or resistance to retinoids, and their endogenous or induced expression can distinguish between these two phenotypes.
Návaznosti
MUNI/A/1586/2018, interní kód MU |
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NV15-34621A, projekt VaV |
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