VACULÍKOVÁ, Anna, Marta FARKOVÁ and Přemysl LUBAL. Application of CD spectroscopy in analytical chemistry. In Libuše Trnková. XIX. Workshop of Biophysical Chemists and Electrochemists. 1st edition. Brno: Masaryk University, 2019, p. 61. ISBN 978-80-210-9309-6.
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Basic information
Original name Application of CD spectroscopy in analytical chemistry
Authors VACULÍKOVÁ, Anna, Marta FARKOVÁ and Přemysl LUBAL.
Edition 1st edition. Brno, XIX. Workshop of Biophysical Chemists and Electrochemists, p. 61-61, 2019.
Publisher Masaryk University
Other information
Original language English
Type of outcome Proceedings paper
Field of Study 10406 Analytical chemistry
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Publication form storage medium (CD, DVD, flash disk)
Organization unit Faculty of Science
ISBN 978-80-210-9309-6
Keywords (in Czech) spectroscopy; circular dichroism; tryptophan
Changed by Changed by: RNDr. Marta Farková, CSc., učo 546. Changed: 27/7/2019 16:38.
Abstract
Many biological compounds exhibit optical activity, which is due to asymmetric carbon on which four different substituents are attached. This configuration leads to two structurally different forms of one substance, when both forms have the ability to rotate the plane of polarized light, one turns to the left side (L-) and the second one to the right one (D-). This phenomena can be monitored by circular dichroism (CD) spectroscopy when the ellipticity is measured in the region of absorption band and this parameter can be used for structural analysis as well as for quantitative determination. However, if L- and D- forms are present in solution in ratio 1:1, i.e. racemic optically inactive mixture, they does not exhibit any rotatation. In our case, the L- and D- forms of tryptophan in aqueous solution were measured. The spectroscopic measurements were done at Jasco J-810 spectropolarimeter (Jasco, Japan) in the wavelength range of 200-300 nm. CD-spectra of pure L- and D-tryptophan and their racemic mixture are presented. It was demonstrated, that CD spectroscopy can be employed for quantitative analysis and determination of purity of chiral aminoacid (Tryptophan).
Links
MUNI/A/1359/2018, interní kód MUName: Vývoj metod a instrumentace pro analýzu biologicky významných látek 2019
Investor: Masaryk University, Category A
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