Multicomponent Yeast Two-Hybrid System: Applications to Study
Protein-Protein Interactions in SMC Complexes.

C 2019

Multicomponent Yeast Two-Hybrid System: Applications to Study Protein-Protein Interactions in SMC Complexes.

PALEČEK, Jan, Lucie VONDROVÁ, Kateřina ZÁBRADY a Jakub OTOČKA

Základní údaje

Originální název

Multicomponent Yeast Two-Hybrid System: Applications to Study Protein-Protein Interactions in SMC Complexes.

Autoři

PALEČEK, Jan (203 Česká republika, garant, domácí), Lucie VONDROVÁ (203 Česká republika, domácí), Kateřina ZÁBRADY (203 Česká republika) a Jakub OTOČKA (703 Slovensko, domácí)

Vydání

New York, NY, Methods in Molecular Biology, od s. 79-90, 12 s. SMC Complexes, vol 2004. 2019

Nakladatel

Springer Nature

Další údaje

Jazyk

angličtina

Typ výsledku

Kapitola resp. kapitoly v odborné knize

Obor

10609 Biochemical research methods

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

tištěná verze "print"

Odkazy

URL

Kód RIV

RIV/00216224:14310/19:00107533

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-1-4939-9519-6

DOI

http://dx.doi.org/10.1007/978-1-4939-9520-2_7

UT WoS

000487933300008

Klíčová slova anglicky

KITE proteins; Kleisin bridge; Multicomponent yeast two-hybrid (Y2H) system; Non-SMC Element (Nse) subunits; Protein–protein interactions; SMC5/6; Structural maintenance of chromosomes (SMC) complexes

Štítky

rivok, topvydavatel

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 1. 4. 2020 21:04, Mgr. Marie Šípková, DiS.

Anotace

V originále

Analysis of protein-protein interactions (PPI) is key for the understanding of most protein assemblies including structural maintenance of chromosomes (SMC) complexes. SMC complexes are composed of SMC proteins, kleisin, and kleisin-interacting subunits. These subunits interact in specific ways to constitute and regulate the closed structure of the complexes. Specifically, kleisin molecules bridge the SMC dimers and the kleisin-interacting subunits modulate stability of the bridge. Here we describe a multicomponent version of a yeast two-hybrid (Y2H) method and its application for analysis of the bridging role of the Nse4 kleisin in the SMC5/6 complex. Using this technique, we also show a stabilizing effect of KITE (kleisin-interacting tandem winged-helix element) proteins on SMC5/6.

Návaznosti

GA18-02067S, projekt VaV

Název: Úloha Nse1/Nse3/Nse4, E3-ubikvitin ligásy, ve stabilitě genomu

Investor: Grantová agentura ČR, Úloha Nse1/Nse3/Nse4, E3-ubikvitin ligásy, ve stabilitě genomu

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

PALEČEK, Jan, Lucie VONDROVÁ, Kateřina ZÁBRADY a Jakub OTOČKA. Multicomponent Yeast Two-Hybrid System: Applications to Study Protein-Protein Interactions in SMC Complexes. In Badrinarayanan A. (eds). Methods in Molecular Biology. New York, NY: Springer Nature, 2019, s. 79-90. SMC Complexes, vol 2004. ISBN 978-1-4939-9519-6. Dostupné z: https://dx.doi.org/10.1007/978-1-4939-9520-2_7.

@inbook{1548183,
   author = {Paleček, Jan and Vondrová, Lucie and Zábrady, Kateřina and Otočka, Jakub},
   address = {New York, NY},
   booktitle = {Methods in Molecular Biology},
   doi = {http://dx.doi.org/10.1007/978-1-4939-9520-2_7},
   editor = {Badrinarayanan A. (eds)},
   keywords = {KITE proteins; Kleisin bridge; Multicomponent yeast two-hybrid (Y2H) system; Non-SMC Element (Nse) subunits; Protein–protein interactions; SMC5/6; Structural maintenance of chromosomes (SMC) complexes},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {New York, NY},
   isbn = {978-1-4939-9519-6},
   pages = {79-90},
   publisher = {Springer Nature},
   title = {Multicomponent Yeast Two-Hybrid System: Applications to Study Protein-Protein Interactions in SMC Complexes.},
   url = {https://link.springer.com/protocol/10.1007%2F978-1-4939-9520-2_7},
   year = {2019}
}

TY  - CHAP
ID  - 1548183
AU  - Paleček, Jan - Vondrová, Lucie - Zábrady, Kateřina - Otočka, Jakub
PY  - 2019
TI  - Multicomponent Yeast Two-Hybrid System: Applications to Study Protein-Protein Interactions in SMC Complexes.
VL  - SMC Complexes, vol 2004.
PB  - Springer Nature
CY  - New York, NY
SN  - 9781493995196
KW  - KITE proteins
KW  - Kleisin bridge
KW  - Multicomponent yeast two-hybrid (Y2H) system
KW  - Non-SMC Element (Nse) subunits
KW  - Protein–protein interactions
KW  - SMC5/6
KW  - Structural maintenance of chromosomes (SMC) complexes
UR  - https://link.springer.com/protocol/10.1007%2F978-1-4939-9520-2_7
L2  - https://link.springer.com/protocol/10.1007%2F978-1-4939-9520-2_7
N2  - Analysis of protein-protein interactions (PPI) is key for the understanding of most protein assemblies including structural maintenance of chromosomes (SMC) complexes. SMC complexes are composed of SMC proteins, kleisin, and kleisin-interacting subunits. These subunits interact in specific ways to constitute and regulate the closed structure of the complexes. Specifically, kleisin molecules bridge the SMC dimers and the kleisin-interacting subunits modulate stability of the bridge. Here we describe a multicomponent version of a yeast two-hybrid (Y2H) method and its application for analysis of the bridging role of the Nse4 kleisin in the SMC5/6 complex. Using this technique, we also show a stabilizing effect of KITE (kleisin-interacting tandem winged-helix element) proteins on SMC5/6.
ER  -

PALEČEK, Jan, Lucie VONDROVÁ, Kateřina ZÁBRADY a Jakub OTOČKA. Multicomponent Yeast Two-Hybrid System: Applications to Study Protein-Protein Interactions in SMC Complexes. In Badrinarayanan A. (eds). \textit{Methods in Molecular Biology}. New York, NY: Springer Nature, 2019, s.~79-90. SMC Complexes, vol 2004. ISBN~978-1-4939-9519-6. Dostupné z: https://dx.doi.org/10.1007/978-1-4939-9520-2\_{}7.

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