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@inbook{1548183, author = {Paleček, Jan and Vondrová, Lucie and Zábrady, Kateřina and Otočka, Jakub}, address = {New York, NY}, booktitle = {Methods in Molecular Biology}, doi = {http://dx.doi.org/10.1007/978-1-4939-9520-2_7}, editor = {Badrinarayanan A. (eds)}, keywords = {KITE proteins; Kleisin bridge; Multicomponent yeast two-hybrid (Y2H) system; Non-SMC Element (Nse) subunits; Protein–protein interactions; SMC5/6; Structural maintenance of chromosomes (SMC) complexes}, howpublished = {tištěná verze "print"}, language = {eng}, location = {New York, NY}, isbn = {978-1-4939-9519-6}, pages = {79-90}, publisher = {Springer Nature}, title = {Multicomponent Yeast Two-Hybrid System: Applications to Study Protein-Protein Interactions in SMC Complexes.}, url = {https://link.springer.com/protocol/10.1007%2F978-1-4939-9520-2_7}, year = {2019} }
TY - CHAP ID - 1548183 AU - Paleček, Jan - Vondrová, Lucie - Zábrady, Kateřina - Otočka, Jakub PY - 2019 TI - Multicomponent Yeast Two-Hybrid System: Applications to Study Protein-Protein Interactions in SMC Complexes. VL - SMC Complexes, vol 2004. PB - Springer Nature CY - New York, NY SN - 9781493995196 KW - KITE proteins KW - Kleisin bridge KW - Multicomponent yeast two-hybrid (Y2H) system KW - Non-SMC Element (Nse) subunits KW - Protein–protein interactions KW - SMC5/6 KW - Structural maintenance of chromosomes (SMC) complexes UR - https://link.springer.com/protocol/10.1007%2F978-1-4939-9520-2_7 L2 - https://link.springer.com/protocol/10.1007%2F978-1-4939-9520-2_7 N2 - Analysis of protein-protein interactions (PPI) is key for the understanding of most protein assemblies including structural maintenance of chromosomes (SMC) complexes. SMC complexes are composed of SMC proteins, kleisin, and kleisin-interacting subunits. These subunits interact in specific ways to constitute and regulate the closed structure of the complexes. Specifically, kleisin molecules bridge the SMC dimers and the kleisin-interacting subunits modulate stability of the bridge. Here we describe a multicomponent version of a yeast two-hybrid (Y2H) method and its application for analysis of the bridging role of the Nse4 kleisin in the SMC5/6 complex. Using this technique, we also show a stabilizing effect of KITE (kleisin-interacting tandem winged-helix element) proteins on SMC5/6. ER -
PALEČEK, Jan, Lucie VONDROVÁ, Kateřina ZÁBRADY a Jakub OTOČKA. Multicomponent Yeast Two-Hybrid System: Applications to Study Protein-Protein Interactions in SMC Complexes. In Badrinarayanan A. (eds). \textit{Methods in Molecular Biology}. New York, NY: Springer Nature, 2019, s.~79-90. SMC Complexes, vol 2004. ISBN~978-1-4939-9519-6. Dostupné z: https://dx.doi.org/10.1007/978-1-4939-9520-2\_{}7.
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