| HORVÁTH, Peter, Peter ŠEBEJ, David KOVÁŘ, Jiří DAMBORSKÝ, Zbyněk PROKOP a Petr KLÁN. Fluorescent pH Indicators for Neutral to Near-Alkaline Conditions Based on 9-Iminopyronin Derivatives. ACS Omega. WASHINGTON: American Chemical Society, 2019, roč. 4, č. 3, s. 5479-5485. ISSN 2470-1343. Dostupné z: https://dx.doi.org/10.1021/acsomega.9b00362. |
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@article{1548608,
author = {Horváth, Peter and Šebej, Peter and Kovář, David and Damborský, Jiří and Prokop, Zbyněk and Klán, Petr},
article_location = {WASHINGTON},
article_number = {3},
doi = {http://dx.doi.org/10.1021/acsomega.9b00362},
keywords = {HALOALKANE DEHALOGENASES; PROBE; FLUOROPHORES; ANALOGS; DYES; QUANTITATION; CELL},
language = {eng},
issn = {2470-1343},
journal = {ACS Omega},
title = {Fluorescent pH Indicators for Neutral to Near-Alkaline Conditions Based on 9-Iminopyronin Derivatives},
url = {https://pubs.acs.org/doi/10.1021/acsomega.9b00362},
volume = {4},
year = {2019}
}
TY - JOUR
ID - 1548608
AU - Horváth, Peter - Šebej, Peter - Kovář, David - Damborský, Jiří - Prokop, Zbyněk - Klán, Petr
PY - 2019
TI - Fluorescent pH Indicators for Neutral to Near-Alkaline Conditions Based on 9-Iminopyronin Derivatives
JF - ACS Omega
VL - 4
IS - 3
SP - 5479-5485
EP - 5479-5485
PB - American Chemical Society
SN - 24701343
KW - HALOALKANE DEHALOGENASES
KW - PROBE
KW - FLUOROPHORES
KW - ANALOGS
KW - DYES
KW - QUANTITATION
KW - CELL
UR - https://pubs.acs.org/doi/10.1021/acsomega.9b00362
L2 - https://pubs.acs.org/doi/10.1021/acsomega.9b00362
N2 - Monitoring enzymatic activities at pH ranges compatible with their physiological optimum using fluorescent assays is important for high-throughput screening and engineering of novel biocatalysts as well as applications in biosensing and diagnostics. However, the number of fluorescent dyes exhibiting necessary optical properties at alkaline pHs is limited. Here, we report the design, synthesis, and biochemical application of fluorescent 9-iminopyronin derivatives possessing unique and distinct emission properties to provide optimal sensitivity at neutral to near-alkaline conditions. They exist in two acid-base forms, allowing for a dual-emission ratiometric sensing with a linear response of pH between 6.0 and 8.5 and detection sensitivity of a 0.06 pH unit. Selected fluorophores were used as the sensors for monitoring the enzymatic hydrolytic activity of a model enzyme haloalkane dehalogenase.
ER -
HORVÁTH, Peter, Peter ŠEBEJ, David KOVÁŘ, Jiří DAMBORSKÝ, Zbyněk PROKOP a Petr KLÁN. Fluorescent pH Indicators for Neutral to Near-Alkaline Conditions Based on 9-Iminopyronin Derivatives. \textit{ACS Omega}. WASHINGTON: American Chemical Society, 2019, roč.~4, č.~3, s.~5479-5485. ISSN~2470-1343. Dostupné z: https://dx.doi.org/10.1021/acsomega.9b00362.
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