HORVÁTH, Peter, Peter ŠEBEJ, David KOVÁŘ, Jiří DAMBORSKÝ, Zbyněk PROKOP a Petr KLÁN. Fluorescent pH Indicators for Neutral to Near-Alkaline Conditions Based on 9-Iminopyronin Derivatives. ACS Omega. WASHINGTON: American Chemical Society, 2019, roč. 4, č. 3, s. 5479-5485. ISSN 2470-1343. Dostupné z: https://dx.doi.org/10.1021/acsomega.9b00362. |
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@article{1548608, author = {Horváth, Peter and Šebej, Peter and Kovář, David and Damborský, Jiří and Prokop, Zbyněk and Klán, Petr}, article_location = {WASHINGTON}, article_number = {3}, doi = {http://dx.doi.org/10.1021/acsomega.9b00362}, keywords = {HALOALKANE DEHALOGENASES; PROBE; FLUOROPHORES; ANALOGS; DYES; QUANTITATION; CELL}, language = {eng}, issn = {2470-1343}, journal = {ACS Omega}, title = {Fluorescent pH Indicators for Neutral to Near-Alkaline Conditions Based on 9-Iminopyronin Derivatives}, url = {https://pubs.acs.org/doi/10.1021/acsomega.9b00362}, volume = {4}, year = {2019} }
TY - JOUR ID - 1548608 AU - Horváth, Peter - Šebej, Peter - Kovář, David - Damborský, Jiří - Prokop, Zbyněk - Klán, Petr PY - 2019 TI - Fluorescent pH Indicators for Neutral to Near-Alkaline Conditions Based on 9-Iminopyronin Derivatives JF - ACS Omega VL - 4 IS - 3 SP - 5479-5485 EP - 5479-5485 PB - American Chemical Society SN - 24701343 KW - HALOALKANE DEHALOGENASES KW - PROBE KW - FLUOROPHORES KW - ANALOGS KW - DYES KW - QUANTITATION KW - CELL UR - https://pubs.acs.org/doi/10.1021/acsomega.9b00362 L2 - https://pubs.acs.org/doi/10.1021/acsomega.9b00362 N2 - Monitoring enzymatic activities at pH ranges compatible with their physiological optimum using fluorescent assays is important for high-throughput screening and engineering of novel biocatalysts as well as applications in biosensing and diagnostics. However, the number of fluorescent dyes exhibiting necessary optical properties at alkaline pHs is limited. Here, we report the design, synthesis, and biochemical application of fluorescent 9-iminopyronin derivatives possessing unique and distinct emission properties to provide optimal sensitivity at neutral to near-alkaline conditions. They exist in two acid-base forms, allowing for a dual-emission ratiometric sensing with a linear response of pH between 6.0 and 8.5 and detection sensitivity of a 0.06 pH unit. Selected fluorophores were used as the sensors for monitoring the enzymatic hydrolytic activity of a model enzyme haloalkane dehalogenase. ER -
HORVÁTH, Peter, Peter ŠEBEJ, David KOVÁŘ, Jiří DAMBORSKÝ, Zbyněk PROKOP a Petr KLÁN. Fluorescent pH Indicators for Neutral to Near-Alkaline Conditions Based on 9-Iminopyronin Derivatives. \textit{ACS Omega}. WASHINGTON: American Chemical Society, 2019, roč.~4, č.~3, s.~5479-5485. ISSN~2470-1343. Dostupné z: https://dx.doi.org/10.1021/acsomega.9b00362.
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