2019
The effect of light wavelength on in vitro bilirubin photodegradation and photoisomer production
VREMAN, Hendrik J., Stephanie KOURULA, Jana JASPROVA, Lucie LUDVÍKOVÁ, Petr KLÁN et. al.Základní údaje
Originální název
The effect of light wavelength on in vitro bilirubin photodegradation and photoisomer production
Autoři
VREMAN, Hendrik J. (840 Spojené státy), Stephanie KOURULA (840 Spojené státy), Jana JASPROVA (203 Česká republika), Lucie LUDVÍKOVÁ (203 Česká republika, domácí), Petr KLÁN (203 Česká republika, garant, domácí), Lucie MUCHOVA (203 Česká republika), Libor VITEK (203 Česká republika), Benjamin K. CLINE (840 Spojené státy), Ronald J. WONG (840 Spojené státy) a David K. STEVENSON (840 Spojené státy)
Vydání
Pediatric Research, Rotterdam, Williams and Wilkins, 2019, 0031-3998
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
30209 Paediatrics
Stát vydavatele
Spojené státy
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Impakt faktor
Impact factor: 2.747
Kód RIV
RIV/00216224:14310/19:00108501
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000466408300024
Klíčová slova anglicky
HUMAN-SERUM ALBUMIN; QUANTUM YIELD; STRUCTURAL PHOTOISOMERIZATION; PHOTOTHERAPY; ISOMERIZATION; DEPENDENCE; ISOMER; TURQUOISE; INFANTS; HYPERBILIRUBINEMIA
Štítky
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 17. 2. 2023 21:18, Mgr. Michaela Hylsová, Ph.D.
Anotace
V originále
BACKGROUND: The action spectrum for bilirubin photodegradation has been intensively studied. However, questions still remain regarding which light wavelength most efficiently photodegrades bilirubin. In this study, we determined the in vitro effects of different irradiation wavelength ranges on bilirubin photodegradation. METHODS: In our in vitro method, normalized absolute irradiance levels of 4.2 x 10(15) photons/cm(2)/s from light-emitting diodes (ranging from 390-530 nm) and 10-nm band-pass filters were used to irradiate bilirubin solutions (25 mg/dL in 4% human serum albumin). Bilirubin and its major photoisomer concentrations were determined; the half-life time of bilirubin (t(1/2)) was calculated for each wavelength range, and the spectral characteristics for bilirubin photodegradation products were obtained for key wavelengths. RESULTS: The in vitro photodegradation of bilirubin at 37 degrees C decreased linearly as the wavelength was increased from 390 to 500 nm with t(1/2) decreasing from 63 to 17 min, respectively. At 460 +/- 10 nm, a significantly lower rate of photodegradation and thus higher t(1/2) (31 min) than that at 500 nm (17 min) was demonstrated. CONCLUSION: In our system, the optimum bilirubin photodegradation and lumirubin production rates occurred between 490 and 500 nm. Spectra shapes were remarkably similar, suggesting that lumirubin production was the major process of bilirubin photodegradation.
Návaznosti
NV18-07-00342, projekt VaV |
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