Developmental toxicity of the fungicide ziram in zebrafish
(Danio rerio)

J 2019

Developmental toxicity of the fungicide ziram in zebrafish (Danio rerio)

CAO, Fangjie, Christopher L. SOUDERS, Pengfei LI, Ondřej ADAMOVSKÝ, Sen PANG et. al.

Základní údaje

Originální název

Developmental toxicity of the fungicide ziram in zebrafish (Danio rerio)

Autoři

CAO, Fangjie (156 Čína), Christopher L. SOUDERS (840 Spojené státy), Pengfei LI (156 Čína), Ondřej ADAMOVSKÝ (203 Česká republika, garant, domácí), Sen PANG (156 Čína), Lihong QIU (156 Čína) a Christopher J. MARTYNIUK (840 Spojené státy)

Vydání

Chemosphere, OXFORD, PERGAMON-ELSEVIER SCIENCE LTD, 2019, 0045-6535

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10511 Environmental sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.778

Kód RIV

RIV/00216224:14310/19:00110370

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.chemosphere.2018.09.105

UT WoS

000449891300035

Klíčová slova anglicky

Ziram; Zebrafish embryos; Mitochondrial bioenergetics; Dark/light preference; Gene expression; Dopamine system

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 3. 2020 09:39, Mgr. Marie Šípková, DiS.

Anotace

V originále

Ziram is a broad spectrum pesticide that belongs to the class of dimethyl-dithiocarbamate (DTC) fungicides. The objectives of this study were to assess the effects of ziram in developing zebrafish. Ziram was highly toxic to zebrafish embryos, with a 96-h LC50 value of 1082.54 nM (similar to 0.33 mg/L). Zebrafish embryos at 6 h post-fertilization (hpf) were exposed to solvent control (0.1% DMSO), or one dose of 1, 10, 100, and 1000 nM ziram for 96 h. Ziram induced lethality in a dose-dependent manner, decreased hatching rate and heartbeat, and caused wavy deformities at 72 and 96 hpf at 100 and 1000 nM. Basal oxygen consumption rates of zebrafish at 24 hpf were decreased with 1000 nM, suggesting that ziram affects oxidative phosphorylation. We also measured the expression of transcripts associated with the oxidative stress response (sod] and sod2) and dopamine receptor signaling at similar to 96 h of exposure. There was no difference in the expression of genes related to oxidative stress, nor those related to the dopamine system. Locomotor activity was also assessed in larval zebrafish (7 dpf), and ziram increased total activity, the velocity in light zone, and total distance moved at 10 nM, while it decreased the mean time spent in the dark zone at 1 and 10 nM. Behavioral responses were dependent upon the time point and clutch examined. These data demonstrate that ziram negatively impacts embryonic development (i.e. mortality, hatching, heartbeat and notochord development) of zebrafish, decreases basal respiration of embryos, and alters behavioral responses in larvae.

Citovat

CAO, Fangjie, Christopher L. SOUDERS, Pengfei LI, Ondřej ADAMOVSKÝ, Sen PANG, Lihong QIU a Christopher J. MARTYNIUK. Developmental toxicity of the fungicide ziram in zebrafish (Danio rerio). Chemosphere. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD, 2019, roč. 214, January, s. 303-313. ISSN 0045-6535. Dostupné z: https://dx.doi.org/10.1016/j.chemosphere.2018.09.105.

@article{1548992,
   author = {Cao, Fangjie and Souders, Christopher L. and Li, Pengfei and Adamovský, Ondřej and Pang, Sen and Qiu, Lihong and Martyniuk, Christopher J.},
   article_location = {OXFORD},
   article_number = {January},
   doi = {http://dx.doi.org/10.1016/j.chemosphere.2018.09.105},
   keywords = {Ziram; Zebrafish embryos; Mitochondrial bioenergetics; Dark/light preference; Gene expression; Dopamine system},
   language = {eng},
   issn = {0045-6535},
   journal = {Chemosphere},
   title = {Developmental toxicity of the fungicide ziram in zebrafish (Danio rerio)},
   url = {https://www.sciencedirect.com/science/article/pii/S0045653518317600?via%3Dihub},
   volume = {214},
   year = {2019}
}

TY  - JOUR
ID  - 1548992
AU  - Cao, Fangjie - Souders, Christopher L. - Li, Pengfei - Adamovský, Ondřej - Pang, Sen - Qiu, Lihong - Martyniuk, Christopher J.
PY  - 2019
TI  - Developmental toxicity of the fungicide ziram in zebrafish (Danio rerio)
JF  - Chemosphere
VL  - 214
IS  - January
SP  - 303-313
EP  - 303-313
PB  - PERGAMON-ELSEVIER SCIENCE LTD
SN  - 00456535
KW  - Ziram
KW  - Zebrafish embryos
KW  - Mitochondrial bioenergetics
KW  - Dark/light preference
KW  - Gene expression
KW  - Dopamine system
UR  - https://www.sciencedirect.com/science/article/pii/S0045653518317600?via%3Dihub
L2  - https://www.sciencedirect.com/science/article/pii/S0045653518317600?via%3Dihub
N2  - Ziram is a broad spectrum pesticide that belongs to the class of dimethyl-dithiocarbamate (DTC) fungicides. The objectives of this study were to assess the effects of ziram in developing zebrafish. Ziram was highly toxic to zebrafish embryos, with a 96-h LC50 value of 1082.54 nM (similar to 0.33 mg/L). Zebrafish embryos at 6 h post-fertilization (hpf) were exposed to solvent control (0.1% DMSO), or one dose of 1, 10, 100, and 1000 nM ziram for 96 h. Ziram induced lethality in a dose-dependent manner, decreased hatching rate and heartbeat, and caused wavy deformities at 72 and 96 hpf at 100 and 1000 nM. Basal oxygen consumption rates of zebrafish at 24 hpf were decreased with 1000 nM, suggesting that ziram affects oxidative phosphorylation. We also measured the expression of transcripts associated with the oxidative stress response (sod] and sod2) and dopamine receptor signaling at similar to 96 h of exposure. There was no difference in the expression of genes related to oxidative stress, nor those related to the dopamine system. Locomotor activity was also assessed in larval zebrafish (7 dpf), and ziram increased total activity, the velocity in light zone, and total distance moved at 10 nM, while it decreased the mean time spent in the dark zone at 1 and 10 nM. Behavioral responses were dependent upon the time point and clutch examined. These data demonstrate that ziram negatively impacts embryonic development (i.e. mortality, hatching, heartbeat and notochord development) of zebrafish, decreases basal respiration of embryos, and alters behavioral responses in larvae.
ER  -

CAO, Fangjie, Christopher L. SOUDERS, Pengfei LI, Ondřej ADAMOVSKÝ, Sen PANG, Lihong QIU a Christopher J. MARTYNIUK. Developmental toxicity of the fungicide ziram in zebrafish (Danio rerio). \textit{Chemosphere}. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD, 2019, roč.~214, January, s.~303-313. ISSN~0045-6535. Dostupné z: https://dx.doi.org/10.1016/j.chemosphere.2018.09.105.

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