Excreted/secreted products of entomopathogenic nematodes and
their effect on insect immunity

a 2019

Excreted/secreted products of entomopathogenic nematodes and their effect on insect immunity

ELIÁŠ, Sara, Jana HURYCHOVÁ, Pavel DOBEŠ, Duarte Nuno TOUBARRO TIAGO, Nelson SIMÕES et. al.

Základní údaje

Originální název

Excreted/secreted products of entomopathogenic nematodes and their effect on insect immunity

Autoři

ELIÁŠ, Sara (276 Německo, domácí), Jana HURYCHOVÁ (203 Česká republika, domácí), Pavel DOBEŠ (203 Česká republika, domácí), Duarte Nuno TOUBARRO TIAGO (620 Portugalsko), Nelson SIMÕES (620 Portugalsko) a Pavel HYRŠL (203 Česká republika, garant, domácí)

Vydání

International Congress on Invertebrate Pathology and Microbial Control & 52nd Annual Meeting of the Society for Invertebrate Pathology & 17th Meeting of the IOBC‐WPRS Working Group “Microbial and Nematode Control of Invertebrate Pests” 2019

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10616 Entomology

Stát vydavatele

Španělsko

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/19:00107567

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Heterorhabditis bacteriophora; phenoloxidase; Galleria mellonella; excreted/secreted products

Změněno: 14. 8. 2019 15:50, Mgr. Sara Šreibr

Anotace

V originále

Entomopathogenic nematodes (EPNs) are the obligate parasites of insects. In the fight against the immune system of the host, nematodes use their excreted/secreted products (ESPs). These products are a mixture of molecules with distinct functions. We identified several functions of the ESPs and examined them in detail - specifically their influence on phenoloxidase activity (PO) and coagulation. ESPs were obtained from two isolates of Heterorhabditis bacteriophora from different environments. Firstly, we purified the samples based on their charge. There were 5 fractions obtained from each isolate of H. b. in which their activity in the inhibition of G. mellonella PO was verified. Inhibition activity was also observed for the coagulation in both isolates. For the active fractions in PO inhibition were used a second purification based on their size. We obtained 18 new fractions in which we found very highly activity which was manifested in the inhibition of phenoloxidase and coagulation. Phenoloxidase and coagulation inhibition were also tested on ESPs isolated from EPN Steinernema carpocapsae. The inhibition of both immune responses was confirmed. Potential use of these results is mainly in the biological control of insect pests.

Návaznosti

GA17-03253S, projekt VaV

Název: Hormonální kontrola hmyzího obranného systému

Investor: Grantová agentura ČR, Hormonální kontrola hmyzího obranného systému

Citovat

ELIÁŠ, Sara, Jana HURYCHOVÁ, Pavel DOBEŠ, Duarte Nuno TOUBARRO TIAGO, Nelson SIMÕES a Pavel HYRŠL. Excreted/secreted products of entomopathogenic nematodes and their effect on insect immunity. In International Congress on Invertebrate Pathology and Microbial Control & 52nd Annual Meeting of the Society for Invertebrate Pathology & 17th Meeting of the IOBC‐WPRS Working Group “Microbial and Nematode Control of Invertebrate Pests”. 2019.

@proceedings{1549357,
   author = {Eliáš, Sara and Hurychová, Jana and Dobeš, Pavel and Toubarro Tiago, Duarte Nuno and Simões, Nelson and Hyršl, Pavel},
   booktitle = {International Congress on Invertebrate Pathology and Microbial Control & 52nd Annual Meeting of the Society for Invertebrate Pathology & 17th Meeting of the IOBC‐WPRS Working Group “Microbial and Nematode Control of Invertebrate Pests”},
   keywords = {Heterorhabditis bacteriophora; phenoloxidase; Galleria mellonella; excreted/secreted products},
   language = {eng},
   title = {Excreted/secreted products of entomopathogenic nematodes and their effect on insect immunity},
   year = {2019}
}

TY  - CONF
ID  - 1549357
AU  - Eliáš, Sara - Hurychová, Jana - Dobeš, Pavel - Toubarro Tiago, Duarte Nuno - Simões, Nelson - Hyršl, Pavel
PY  - 2019
TI  - Excreted/secreted products of entomopathogenic nematodes and their effect on insect immunity
KW  - Heterorhabditis bacteriophora
KW  - phenoloxidase
KW  - Galleria mellonella
KW  - excreted/secreted products
N2  - Entomopathogenic nematodes (EPNs) are the obligate parasites of insects. In the fight against the immune system of the host, nematodes use their excreted/secreted products (ESPs). These products are a mixture of molecules with distinct functions. We identified several functions of the ESPs and examined them in detail - specifically their influence on phenoloxidase activity (PO) and coagulation. ESPs were obtained from two isolates of Heterorhabditis bacteriophora from different environments. Firstly, we purified the samples based on their charge. There were 5 fractions obtained from each isolate of H. b. in which their activity in the inhibition of G. mellonella PO was verified. Inhibition activity was also observed for the coagulation in both isolates. For the active fractions in PO inhibition were used a second purification based on their size. We obtained 18 new fractions in which we found very highly activity which was manifested in the inhibition of phenoloxidase and coagulation. Phenoloxidase and coagulation inhibition were also tested on ESPs isolated from EPN Steinernema carpocapsae. The inhibition of both immune responses was confirmed. Potential use of these results is mainly in the biological control of insect pests.
ER  -

ELIÁŠ, Sara, Jana HURYCHOVÁ, Pavel DOBEŠ, Duarte Nuno TOUBARRO TIAGO, Nelson SIMÕES a Pavel HYRŠL. Excreted/secreted products of entomopathogenic nematodes and their effect on insect immunity. In \textit{International Congress on Invertebrate Pathology and Microbial Control \&{} 52nd Annual Meeting of the Society for Invertebrate Pathology \&{} 17th Meeting of the IOBC‐WPRS Working Group “Microbial and Nematode Control of Invertebrate Pests”}. 2019.

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