Microscopy examination of red blood and yeast cell
agglutination induced by bacterial lectins

J 2019

Microscopy examination of red blood and yeast cell agglutination induced by bacterial lectins

MRÁZKOVÁ, Jana, Lenka MALINOVSKÁ a Michaela WIMMEROVÁ

Základní údaje

Originální název

Microscopy examination of red blood and yeast cell agglutination induced by bacterial lectins

Autoři

MRÁZKOVÁ, Jana (203 Česká republika, domácí), Lenka MALINOVSKÁ (203 Česká republika, domácí) a Michaela WIMMEROVÁ (203 Česká republika, garant, domácí)

Vydání

PLOS ONE, Public Library of Science, 2019, 1932-6203

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.740

Kód RIV

RIV/00216224:14740/19:00107577

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1371/journal.pone.0220318

UT WoS

000484977900081

Klíčová slova anglicky

lectins; microscopy; agglutination; specificity

Štítky

CF BIC, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 27. 10. 2024 15:04, Ing. Martina Blahová

Anotace

V originále

Lectins are a group of ubiquitous proteins which specifically recognize and reversibly bind sugar moieties of glycoprotein and glycolipid constituents on cell surfaces. The mutagenesis approach is often employed to characterize lectin binding properties. As lectins are not enzymes, it is not easy to perform a rapid specificity screening of mutants using chromogenic substrates. Lectin-based cell agglutination is a simple and low-cost technique which can overcome most of these problems. In this work, a modified method of the agglutination of human erythrocytes and yeast cells with microscopy detection was successfully used for a specificity study of the newly prepared mutant lectin RS-IIL_A22S, which experimentally completed studies on sugar preferences of lectins in the PA-IIL family. Results showed that the sensitivity of this method is comparable with ITC, is able to determine subtle differences in lectin specificity, and works directly in cell lysates.

Návaznosti

GA18-18964S, projekt VaV

Název: Lektiny a jejich úloha v interakci patogen/hostitel a buněčném rozpoznávání

Investor: Grantová agentura ČR, Lektiny a jejich úloha v interakci patogen/hostitel a buněčném rozpoznávání

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

MUNI/A/1575/2018, interní kód MU

Název: Podpora biochemického výzkumu v roce 2019

Investor: Masarykova univerzita, Podpora biochemického výzkumu v roce 2019, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

90043, velká výzkumná infrastruktura

Název: CIISB

Citovat

MRÁZKOVÁ, Jana, Lenka MALINOVSKÁ a Michaela WIMMEROVÁ. Microscopy examination of red blood and yeast cell agglutination induced by bacterial lectins. PLOS ONE. Public Library of Science, 2019, roč. 14, č. 7, s. 1-23. ISSN 1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0220318.

@article{1549857,
   author = {Mrázková, Jana and Malinovská, Lenka and Wimmerová, Michaela},
   article_number = {7},
   doi = {http://dx.doi.org/10.1371/journal.pone.0220318},
   keywords = {lectins; microscopy; agglutination; specificity},
   language = {eng},
   issn = {1932-6203},
   journal = {PLOS ONE},
   title = {Microscopy examination of red blood and yeast cell agglutination induced by bacterial lectins},
   url = {https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0220318&type=printable},
   volume = {14},
   year = {2019}
}

TY  - JOUR
ID  - 1549857
AU  - Mrázková, Jana - Malinovská, Lenka - Wimmerová, Michaela
PY  - 2019
TI  - Microscopy examination of red blood and yeast cell agglutination induced by bacterial lectins
JF  - PLOS ONE
VL  - 14
IS  - 7
SP  - 1-23
EP  - 1-23
PB  - Public Library of Science
SN  - 19326203
KW  - lectins
KW  - microscopy
KW  - agglutination
KW  - specificity
UR  - https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0220318&type=printable
L2  - https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0220318&type=printable
N2  - Lectins are a group of ubiquitous proteins which specifically recognize and reversibly bind sugar moieties of glycoprotein and glycolipid constituents on cell surfaces. The mutagenesis approach is often employed to characterize lectin binding properties. As lectins are not enzymes, it is not easy to perform a rapid specificity screening of mutants using chromogenic substrates. Lectin-based cell agglutination is a simple and low-cost technique which can overcome most of these problems. In this work, a modified method of the agglutination of human erythrocytes and yeast cells with microscopy detection was successfully used for a specificity study of the newly prepared mutant lectin RS-IIL_A22S, which experimentally completed studies on sugar preferences of lectins in the PA-IIL family. Results showed that the sensitivity of this method is comparable with ITC, is able to determine subtle differences in lectin specificity, and works directly in cell lysates.
ER  -

MRÁZKOVÁ, Jana, Lenka MALINOVSKÁ a Michaela WIMMEROVÁ. Microscopy examination of red blood and yeast cell agglutination induced by bacterial lectins. \textit{PLOS ONE}. Public Library of Science, 2019, roč.~14, č.~7, s.~1-23. ISSN~1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0220318.

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