Impact of acceptor splice site NAGTAG motif on exon recognition

J 2019

Impact of acceptor splice site NAGTAG motif on exon recognition

HUJOVÁ, Pavla, Lucie GRODECKÁ, Přemysl SOUČEK a Tomáš FREIBERGER

Základní údaje

Originální název

Impact of acceptor splice site NAGTAG motif on exon recognition

Autoři

HUJOVÁ, Pavla (203 Česká republika, domácí), Lucie GRODECKÁ (203 Česká republika), Přemysl SOUČEK (203 Česká republika, garant) a Tomáš FREIBERGER (203 Česká republika, domácí)

Vydání

MOLECULAR BIOLOGY REPORTS, DORDRECHT, Springer, 2019, 0301-4851

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.402

Kód RIV

RIV/00216224:14110/19:00108505

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1007/s11033-019-04734-6

UT WoS

000470332600028

Klíčová slova anglicky

Pre-mRNA splicing; Acceptor splice site; NAGNAG motif; Tandem acceptor splice site

Štítky

14110114, rivok

Změněno: 18. 12. 2019 13:24, Mgr. Tereza Miškechová

Anotace

V originále

Pre-mRNA splicing is an essential step in gene expression, when introns are removed and exons joined by the complex of proteins called spliceosome. Correct splicing requires a precise exon/intron junction definition, which is determined by a consensual donor and acceptor splice site at the 5 and 3 end, respectively. An acceptor splice site (3ss) consists of highly conserved AG nucleotides in positions E-2 and E-1. These nucleotides can appear in tandem, located 3bp from each other. Then they are referred to as NAGNAG or tandem 3ss, which can be alternatively spliced. NAG/TAG 3ss motif abundance is extremely low and cannot be easily explained by just a nucleotide preference in this position. We tested artificial NAG/TAG motif's potential negative effect on exon recognition using a minigene assay. Introducing the NAG/TAG motif into seven different exons revealed no general negative effect on exon recognition. The only observed effect was the partial use of the newly formed distal 3ss. We can conclude that this motif's extremely low preference in a natural 3ss is not a consequence of the NAG/TAG motif's negative effect on exon recognition, but more likely the result of other RNA processing aspects, such as an alternative 3ss choice, decreased 3ss strength, or incorporating an amber stop codon.

Návaznosti

NV16-34414A, projekt VaV

Název: Určení genových oblastí náchylných ke vzniku mutací ovlivňujících sestřih mRNA

Citovat

HUJOVÁ, Pavla, Lucie GRODECKÁ, Přemysl SOUČEK a Tomáš FREIBERGER. Impact of acceptor splice site NAGTAG motif on exon recognition. MOLECULAR BIOLOGY REPORTS. DORDRECHT: Springer, 2019, roč. 46, č. 3, s. 2877-2884. ISSN 0301-4851. Dostupné z: https://dx.doi.org/10.1007/s11033-019-04734-6.

@article{1550501,
   author = {Hujová, Pavla and Grodecká, Lucie and Souček, Přemysl and Freiberger, Tomáš},
   article_location = {DORDRECHT},
   article_number = {3},
   doi = {http://dx.doi.org/10.1007/s11033-019-04734-6},
   keywords = {Pre-mRNA splicing; Acceptor splice site; NAGNAG motif; Tandem acceptor splice site},
   language = {eng},
   issn = {0301-4851},
   journal = {MOLECULAR BIOLOGY REPORTS},
   title = {Impact of acceptor splice site NAGTAG motif on exon recognition},
   url = {http://dx.doi.org/10.1007/s11033-019-04734-6},
   volume = {46},
   year = {2019}
}

TY  - JOUR
ID  - 1550501
AU  - Hujová, Pavla - Grodecká, Lucie - Souček, Přemysl - Freiberger, Tomáš
PY  - 2019
TI  - Impact of acceptor splice site NAGTAG motif on exon recognition
JF  - MOLECULAR BIOLOGY REPORTS
VL  - 46
IS  - 3
SP  - 2877-2884
EP  - 2877-2884
PB  - Springer
SN  - 03014851
KW  - Pre-mRNA splicing
KW  - Acceptor splice site
KW  - NAGNAG motif
KW  - Tandem acceptor splice site
UR  - http://dx.doi.org/10.1007/s11033-019-04734-6
L2  - http://dx.doi.org/10.1007/s11033-019-04734-6
N2  - Pre-mRNA splicing is an essential step in gene expression, when introns are removed and exons joined by the complex of proteins called spliceosome. Correct splicing requires a precise exon/intron junction definition, which is determined by a consensual donor and acceptor splice site at the 5 and 3 end, respectively. An acceptor splice site (3ss) consists of highly conserved AG nucleotides in positions E-2 and E-1. These nucleotides can appear in tandem, located 3bp from each other. Then they are referred to as NAGNAG or tandem 3ss, which can be alternatively spliced. NAG/TAG 3ss motif abundance is extremely low and cannot be easily explained by just a nucleotide preference in this position. We tested artificial NAG/TAG motif's potential negative effect on exon recognition using a minigene assay. Introducing the NAG/TAG motif into seven different exons revealed no general negative effect on exon recognition. The only observed effect was the partial use of the newly formed distal 3ss. We can conclude that this motif's extremely low preference in a natural 3ss is not a consequence of the NAG/TAG motif's negative effect on exon recognition, but more likely the result of other RNA processing aspects, such as an alternative 3ss choice, decreased 3ss strength, or incorporating an amber stop codon.
ER  -

HUJOVÁ, Pavla, Lucie GRODECKÁ, Přemysl SOUČEK a Tomáš FREIBERGER. Impact of acceptor splice site NAGTAG motif on exon recognition. \textit{MOLECULAR BIOLOGY REPORTS}. DORDRECHT: Springer, 2019, roč.~46, č.~3, s.~2877-2884. ISSN~0301-4851. Dostupné z: https://dx.doi.org/10.1007/s11033-019-04734-6.

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