Impact of acceptor splice site NAGTAG motif on exon recognition

HUJOVÁ, Pavla, Lucie GRODECKÁ, Přemysl SOUČEK and Tomáš FREIBERGER. Impact of acceptor splice site NAGTAG motif on exon recognition. MOLECULAR BIOLOGY REPORTS. DORDRECHT: Springer, 2019, vol. 46, No 3, p. 2877-2884. ISSN 0301-4851. Available from: https://dx.doi.org/10.1007/s11033-019-04734-6.

Basic information

• Original name: Impact of acceptor splice site NAGTAG motif on exon recognition
• Authors: HUJOVÁ, Pavla (203 Czech Republic, belonging to the institution), Lucie GRODECKÁ (203 Czech Republic), Přemysl SOUČEK (203 Czech Republic, guarantor) and Tomáš FREIBERGER (203 Czech Republic, belonging to the institution).
• Edition: MOLECULAR BIOLOGY REPORTS, DORDRECHT, Springer, 2019, 0301-4851.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10608 Biochemistry and molecular biology
• Country of publisher: Netherlands
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 1.402
• RIV identification code: RIV/00216224:14110/19:00108505
• Organization unit: Faculty of Medicine
• Doi: http://dx.doi.org/10.1007/s11033-019-04734-6
• UT WoS: 000470332600028
• Keywords in English: Pre-mRNA splicing; Acceptor splice site; NAGNAG motif; Tandem acceptor splice site
• Tags: 14110114, rivok

Abstract

Pre-mRNA splicing is an essential step in gene expression, when introns are removed and exons joined by the complex of proteins called spliceosome. Correct splicing requires a precise exon/intron junction definition, which is determined by a consensual donor and acceptor splice site at the 5 and 3 end, respectively. An acceptor splice site (3ss) consists of highly conserved AG nucleotides in positions E-2 and E-1. These nucleotides can appear in tandem, located 3bp from each other. Then they are referred to as NAGNAG or tandem 3ss, which can be alternatively spliced. NAG/TAG 3ss motif abundance is extremely low and cannot be easily explained by just a nucleotide preference in this position. We tested artificial NAG/TAG motif's potential negative effect on exon recognition using a minigene assay. Introducing the NAG/TAG motif into seven different exons revealed no general negative effect on exon recognition. The only observed effect was the partial use of the newly formed distal 3ss. We can conclude that this motif's extremely low preference in a natural 3ss is not a consequence of the NAG/TAG motif's negative effect on exon recognition, but more likely the result of other RNA processing aspects, such as an alternative 3ss choice, decreased 3ss strength, or incorporating an amber stop codon.

Links

• NV16-34414A, research and development project: Name: Určení genových oblastí náchylných ke vzniku mutací ovlivňujících sestřih mRNA