Egg maturity assessment prior to ICSI prevents premature
fertilization of late-maturing oocytes

J 2019

Egg maturity assessment prior to ICSI prevents premature fertilization of late-maturing oocytes

HOLUBCOVÁ, Zuzana, Drahomíra KYJOVSKÁ, Martina MARTONOVÁ, Darja PÁRALOVÁ, Tereza KLENKOVÁ et. al.

Základní údaje

Originální název

Egg maturity assessment prior to ICSI prevents premature fertilization of late-maturing oocytes

Autoři

HOLUBCOVÁ, Zuzana (203 Česká republika, garant, domácí), Drahomíra KYJOVSKÁ (203 Česká republika), Martina MARTONOVÁ (203 Česká republika), Darja PÁRALOVÁ (203 Česká republika), Tereza KLENKOVÁ (203 Česká republika), Pavel OTEVŘEL (203 Česká republika), Radka ŠTĚPÁNOVÁ (203 Česká republika, domácí), Soňa KLOUDOVÁ (203 Česká republika) a Aleš HAMPL (203 Česká republika, domácí)

Vydání

Journal of Assisted Reproduction and Genetics, NEW YORK, SPRINGER/PLENUM PUBLISHERS, 2019, 1058-0468

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30214 Obstetrics and gynaecology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.829

Kód RIV

RIV/00216224:14110/19:00110498

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1007/s10815-018-1393-0

UT WoS

000463773900011

Klíčová slova anglicky

Timing of ICSI; Oocyte maturation; Polarized light microscopy; Meiotic spindle; Immature oocytes

Štítky

14110516, 14110517, CF CELLIM, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 14. 4. 2020 14:21, Mgr. Tereza Miškechová

Anotace

V originále

Propose The presence of metaphase II (MII) spindle together with the polar body (PB) indicates completion of oocyte maturation. This study was designed to explore if spindle imaging can be used to optimize timing of intracytoplasmic sperm injection (ICSI). Methods The study involved 916 oocytes from 234 conventionally stimulated ICSI cycles with an unexpectedly poor ovarian response. All PB-displaying oocytes were subjected to polarized light microscopy (PLM) prior to ICSI. When MII spindle was absent in the majority of oocytes, ICSI was postponed and performed after additional spindle imaging. Fertilization, embryo development, and clinical outcome were evaluated with respect to the observed spindle pattern. Results The visible spindle was absent in 32.64% of PB-displaying oocytes. The late-maturing oocytes extruding PB in vitro were less likely to exhibit a spindle signal than in vivo matured MII oocytes (38.86% vs. 89.84%). When fertilization was postponed, 59.39% of initially spindle-negative oocytes developed detectable MII spindle. Spindled eggs had significantly higher developmental potential, and the presence of the spindle has been identified as an independent measure for predicting the formation of the blastocyst. Embryos derived from spindle-positive oocytes also showed a higher chance to implant and develop to term. Notably, 11 children were conceived by finely timed fertilization of late-maturing oocytes which are normally discarded. Conclusions The study confirms the prognostic value of spindle imaging and demonstrates that immature oocytes can be clinically utilized and give rise to live births when the timing of ICSI is adjusted to their developmental stage.

Citovat

HOLUBCOVÁ, Zuzana, Drahomíra KYJOVSKÁ, Martina MARTONOVÁ, Darja PÁRALOVÁ, Tereza KLENKOVÁ, Pavel OTEVŘEL, Radka ŠTĚPÁNOVÁ, Soňa KLOUDOVÁ a Aleš HAMPL. Egg maturity assessment prior to ICSI prevents premature fertilization of late-maturing oocytes. Journal of Assisted Reproduction and Genetics. NEW YORK: SPRINGER/PLENUM PUBLISHERS, 2019, roč. 36, č. 3, s. 445-452. ISSN 1058-0468. Dostupné z: https://dx.doi.org/10.1007/s10815-018-1393-0.

@article{1550537,
   author = {Holubcová, Zuzana and Kyjovská, Drahomíra and Martonová, Martina and Páralová, Darja and Klenková, Tereza and Otevřel, Pavel and Štěpánová, Radka and Kloudová, Soňa and Hampl, Aleš},
   article_location = {NEW YORK},
   article_number = {3},
   doi = {http://dx.doi.org/10.1007/s10815-018-1393-0},
   keywords = {Timing of ICSI; Oocyte maturation; Polarized light microscopy; Meiotic spindle; Immature oocytes},
   language = {eng},
   issn = {1058-0468},
   journal = {Journal of Assisted Reproduction and Genetics},
   title = {Egg maturity assessment prior to ICSI prevents premature fertilization of late-maturing oocytes},
   url = {https://link.springer.com/article/10.1007%2Fs10815-018-1393-0},
   volume = {36},
   year = {2019}
}

TY  - JOUR
ID  - 1550537
AU  - Holubcová, Zuzana - Kyjovská, Drahomíra - Martonová, Martina - Páralová, Darja - Klenková, Tereza - Otevřel, Pavel - Štěpánová, Radka - Kloudová, Soňa - Hampl, Aleš
PY  - 2019
TI  - Egg maturity assessment prior to ICSI prevents premature fertilization of late-maturing oocytes
JF  - Journal of Assisted Reproduction and Genetics
VL  - 36
IS  - 3
SP  - 445-452
EP  - 445-452
PB  - SPRINGER/PLENUM PUBLISHERS
SN  - 10580468
KW  - Timing of ICSI
KW  - Oocyte maturation
KW  - Polarized light microscopy
KW  - Meiotic spindle
KW  - Immature oocytes
UR  - https://link.springer.com/article/10.1007%2Fs10815-018-1393-0
L2  - https://link.springer.com/article/10.1007%2Fs10815-018-1393-0
N2  - Propose The presence of metaphase II (MII) spindle together with the polar body (PB) indicates completion of oocyte maturation. This study was designed to explore if spindle imaging can be used to optimize timing of intracytoplasmic sperm injection (ICSI). Methods The study involved 916 oocytes from 234 conventionally stimulated ICSI cycles with an unexpectedly poor ovarian response. All PB-displaying oocytes were subjected to polarized light microscopy (PLM) prior to ICSI. When MII spindle was absent in the majority of oocytes, ICSI was postponed and performed after additional spindle imaging. Fertilization, embryo development, and clinical outcome were evaluated with respect to the observed spindle pattern. Results The visible spindle was absent in 32.64% of PB-displaying oocytes. The late-maturing oocytes extruding PB in vitro were less likely to exhibit a spindle signal than in vivo matured MII oocytes (38.86% vs. 89.84%). When fertilization was postponed, 59.39% of initially spindle-negative oocytes developed detectable MII spindle. Spindled eggs had significantly higher developmental potential, and the presence of the spindle has been identified as an independent measure for predicting the formation of the blastocyst. Embryos derived from spindle-positive oocytes also showed a higher chance to implant and develop to term. Notably, 11 children were conceived by finely timed fertilization of late-maturing oocytes which are normally discarded. Conclusions The study confirms the prognostic value of spindle imaging and demonstrates that immature oocytes can be clinically utilized and give rise to live births when the timing of ICSI is adjusted to their developmental stage.
ER  -

HOLUBCOVÁ, Zuzana, Drahomíra KYJOVSKÁ, Martina MARTONOVÁ, Darja PÁRALOVÁ, Tereza KLENKOVÁ, Pavel OTEVŘEL, Radka ŠTĚPÁNOVÁ, Soňa KLOUDOVÁ a Aleš HAMPL. Egg maturity assessment prior to ICSI prevents premature fertilization of late-maturing oocytes. \textit{Journal of Assisted Reproduction and Genetics}. NEW YORK: SPRINGER/PLENUM PUBLISHERS, 2019, roč.~36, č.~3, s.~445-452. ISSN~1058-0468. Dostupné z: https://dx.doi.org/10.1007/s10815-018-1393-0.

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