| ONDIČ, Ondrej, Jana NĚMCOVÁ, Reza ALAGHEHBANDAN, Kateřina ČERNÁ, Barbora GOMOLČÁKOVÁ, Iva KINKOROVÁ-LUŇÁČKOVÁ, Jan CHYTRA, Henrieta ŠIDLOVÁ, Ondřej MÁJEK a Jiří BOUDA. The detection of DNA methylation of tumour suppressor genes in cervical high-grade squamous intraepithelial lesion: A prospective cytological-histological correlation study of 70 cases. CYTOPATHOLOGY. HOBOKEN: WILEY, 2019, roč. 30, č. 4, s. 426-431. ISSN 0956-5507. Dostupné z: https://dx.doi.org/10.1111/cyt.12718. |
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@article{1552438,
author = {Ondič, Ondrej and Němcová, Jana and Alaghehbandan, Reza and Černá, Kateřina and Gomolčáková, Barbora and KinkorováandLuňáčková, Iva and Chytra, Jan and Šidlová, Henrieta and Májek, Ondřej and Bouda, Jiří},
article_location = {HOBOKEN},
article_number = {4},
doi = {http://dx.doi.org/10.1111/cyt.12718},
keywords = {cervix; cytology; HSIL; hypermethylation; methylation},
language = {eng},
issn = {0956-5507},
journal = {CYTOPATHOLOGY},
title = {The detection of DNA methylation of tumour suppressor genes in cervical high-grade squamous intraepithelial lesion: A prospective cytological-histological correlation study of 70 cases},
url = {http://dx.doi.org/10.1111/cyt.12718},
volume = {30},
year = {2019}
}
TY - JOUR
ID - 1552438
AU - Ondič, Ondrej - Němcová, Jana - Alaghehbandan, Reza - Černá, Kateřina - Gomolčáková, Barbora - Kinkorová-Luňáčková, Iva - Chytra, Jan - Šidlová, Henrieta - Májek, Ondřej - Bouda, Jiří
PY - 2019
TI - The detection of DNA methylation of tumour suppressor genes in cervical high-grade squamous intraepithelial lesion: A prospective cytological-histological correlation study of 70 cases
JF - CYTOPATHOLOGY
VL - 30
IS - 4
SP - 426-431
EP - 426-431
PB - WILEY
SN - 09565507
KW - cervix
KW - cytology
KW - HSIL
KW - hypermethylation
KW - methylation
UR - http://dx.doi.org/10.1111/cyt.12718
L2 - http://dx.doi.org/10.1111/cyt.12718
N2 - Background DNA methylation has been suggested as one of the epigenetic changes promoting carcinogenesis. The aim of this study was to prospectively evaluate the methylation status of CADM 1, MAL and hsa-miR-124 genes in high-grade squamous intraepithelial lesion (HSIL) liquid-based cytology (LBC) samples with a histological correlation. Methods Seventy histologically confirmed cases of HSIL paired with prior screening LBC diagnosis of HSIL within a 3-month interval were selected. Histologically, the lesions were reviewed and assessed including: (a) number of blocks harbouring dysplastic squamous epithelium; (b) number of blocks containing glandular extension of dysplastic epithelium; and (c) the depth of glandular extension (which was assessed semi-quantitatively as graded 1-3). Human papillomavirus (HPV) subtyping was performed from residual LBC materials using the LINEAR ARRAY HPV Genotyping Test and in-house polymerase chain reaction targeting the HPV E1 gene. The detection of methylation silencing of tumour suppressor genes CADM1, MAL and hsa-miR-124 was performed by multiplex methylation-specific real-time polymerase chain reaction. Results A positive methylation status was detected in 41 cases (58.6%). The number of blocks with HSIL varied from one to 13. Glandular extension was seen in 44 cases with the number of blocks involved ranging from one to 10. The depth of HSIL glandular extension varied. Conclusion The DNA methylation test allows HSIL lesions to be divided into two distinct groups of methylated HSIL in significantly older patients and unmethylated HSIL in younger patients. This study was not able to prove that methylation status in cervical HSIL correlates with the size of the lesion (measured by the number of blocks involved) or with HSIL propensity for endocervical glandular extension, nor with HPV type or multi-infection.
ER -
ONDIČ, Ondrej, Jana NĚMCOVÁ, Reza ALAGHEHBANDAN, Kateřina ČERNÁ, Barbora GOMOLČÁKOVÁ, Iva KINKOROVÁ-LUŇÁČKOVÁ, Jan CHYTRA, Henrieta ŠIDLOVÁ, Ondřej MÁJEK a Jiří BOUDA. The detection of DNA methylation of tumour suppressor genes in cervical high-grade squamous intraepithelial lesion: A prospective cytological-histological correlation study of 70 cases. \textit{CYTOPATHOLOGY}. HOBOKEN: WILEY, 2019, roč.~30, č.~4, s.~426-431. ISSN~0956-5507. Dostupné z: https://dx.doi.org/10.1111/cyt.12718.
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