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@proceedings{1552838,
author = {Killinger, Michael and Buchtová, marcela},
booktitle = {International Congress of Vertebrate Morphology.},
keywords = {morphology},
language = {eng},
title = {PORCN inhibition stimulates chondrocyte differentiation},
url = {https://onlinelibrary.wiley.com/toc/10974687/2019/280/S1},
year = {2019}
}
TY - CONF
ID - 1552838
AU - Killinger, Michael - Buchtová, marcela
PY - 2019
TI - PORCN inhibition stimulates chondrocyte differentiation
KW - morphology
UR - https://onlinelibrary.wiley.com/toc/10974687/2019/280/S1
L2 - https://onlinelibrary.wiley.com/toc/10974687/2019/280/S1
N2 - Porcupine (PORCN) is an endoplasmic reticulum protein belonging tothe membrane bound O–acyl transferase superfamily. This molecule isnecessary for the attachment of long chain fatty acids to WNT pro-teins. PORCN is expressed in most tissues of the body and animalswith loss of function in PORCN exhibit embryonic lethality at earlydevelopmental stages with extensive gastrulation defects. Severaldominant mutations in PORCN were described in human patients,who demonstrated significant skeletal abnormalities. Recently, a pow-erful PORCN inhibitor C59 has become commercially available andhas been successfully used to inhibit WNT pathway in several cancerlines. C59 negatively affects WNT signaling by inhibition of WNTpalmitoylation, which is necessary to acetylate WNT ligands beforetheir secretion and binding to a carrier protein. Here, we analyzed thepossible effect of this inhibitor on skeletogenesis with focus on endo-chondral bone formation. We observed, enhanced growth of mesen-chymal condensations and also increased number of cartilage nodulesin primary micromass cultures established from the early limb bud,which were treated by increasing concentrations of C59.
ER -
KILLINGER, Michael a marcela BUCHTOVÁ. PORCN inhibition stimulates chondrocyte differentiation. In \textit{International Congress of Vertebrate Morphology.}. 2019. ISSN~0362-2525.
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