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@proceedings{1553916,
author = {Michalcová, Lenka and Nevídalová, Hana and Glatz, Zdeněk},
booktitle = {26th International Symposium on Electro- and Liquid Phase-Separation Techniques (ITP2019)},
title = {Development of new on-line CE-FA methods for binding studies},
year = {2019}
}
TY - CONF
ID - 1553916
AU - Michalcová, Lenka - Nevídalová, Hana - Glatz, Zdeněk
PY - 2019
TI - Development of new on-line CE-FA methods for binding studies
N2 - In this study, the new on-line CE-FA method was created. The on-line approach of the sample mixing directly inside the capillary is based on diffusion and it is generic, robust and rapid. The numerous injection parameters and incubation period were considered and optimized. The method was used to determine the binding parameters of model systems (propranolol/lidocaine BSA) and compared with the classic off-line methods. Based on the statistical evaluation, it was found that the results achieved both on-line and off-line methods are almost identical. On-line sample mixing for the CE FA method has so far not been published and a combination of CE FA approaches gives rise to a fully automated and versatile method for characterizing binding interactions.
ER -
MICHALCOVÁ, Lenka, Hana NEVÍDALOVÁ a Zdeněk GLATZ. Development of new on-line CE-FA methods for binding studies. In \textit{26th International Symposium on Electro- and Liquid Phase-Separation Techniques (ITP2019)}. 2019.
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