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@proceedings{1554077,
author = {Modlitbová, Pavlína and Farka, Zdeněk and Pastucha, Matěj and Pořízka, Pavel and Novotný, Karel and Skládal, Petr and Kaiser, Jozef},
booktitle = {EMSLIBS 2019: 10th Euro-Mediterranean Symposium on Laser-Induced Breakdown Spectroscopy},
language = {eng},
isbn = {978-80-88195-13-9},
title = {Laser-Induced Breakdown Spectroscopy as a Novel Readout Method for Nanoparticle-Based Immunoassays},
url = {https://emslibs.com/wp-content/uploads/2019/09/b3.2.pdf},
year = {2019}
}
TY - CONF
ID - 1554077
AU - Modlitbová, Pavlína - Farka, Zdeněk - Pastucha, Matěj - Pořízka, Pavel - Novotný, Karel - Skládal, Petr - Kaiser, Jozef
PY - 2019
TI - Laser-Induced Breakdown Spectroscopy as a Novel Readout Method for Nanoparticle-Based Immunoassays
SN - 9788088195139
UR - https://emslibs.com/wp-content/uploads/2019/09/b3.2.pdf
N2 - Laser-Induced Breakdown Spectroscopy (LIBS) was examined as a novel method to provide the readout of microtiter plate immunoassays involving nanoparticles (NP). The so-called Tag-LIBS, i.e. a sensitive detection of specific biomarkers, was applied to detect the NP labels using the nanosecond ablation sampling. The NP labels were examined from the bottom of the standard 96-well microtiter plate. Thanks to the well-known flexibility in the LIBS instrumentation, the plasma emission collection as well as the focusing optics can be rebuilt into a collinear arrangement. The presented experiments proved the suitable detection of silver NPs from the microtiter plate’s bottom without any negative effects on the NPs’ qualitative and quantitative analysis. Subsequently, a sandwich immunoassay tha tdetects human serum albumin was successfully developed, using streptavidin-coated AgNP labels. The assay provided a detection limit of 10 ng/mL, which is comparable with the fluorescence-based readout. The main advantage of LIBS readout is that it enables a detection of any type of NP labels independent on their fluorescence or catalytic properties. Owing to the immediate signal response, a relatively simple instrumentation enabling the automation, and the capability of multi-elemental analyses, LIBS technique offers a promising and fast alternative to other readout techniques allowing a multiplex detection of various biomarkers.
ER -
MODLITBOVÁ, Pavlína, Zdeněk FARKA, Matěj PASTUCHA, Pavel POŘÍZKA, Karel NOVOTNÝ, Petr SKLÁDAL a Jozef KAISER. Laser-Induced Breakdown Spectroscopy as a Novel Readout Method for Nanoparticle-Based Immunoassays. In \textit{EMSLIBS 2019: 10th Euro-Mediterranean Symposium on Laser-Induced Breakdown Spectroscopy}. 2019. ISBN~978-80-88195-13-9.
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