Expression of sodium channel NaV 1.8 in dorsal root ganglia
after Paclitaxel treatment

k 2019

Expression of sodium channel NaV 1.8 in dorsal root ganglia after Paclitaxel treatment

LEVIN, Shahaf, Petr DUBOVÝ a Marek JOUKAL

Základní údaje

Originální název

Expression of sodium channel NaV 1.8 in dorsal root ganglia after Paclitaxel treatment

Autoři

LEVIN, Shahaf (376 Izrael, domácí), Petr DUBOVÝ (203 Česká republika, domácí) a Marek JOUKAL (203 Česká republika, garant, domácí)

Vydání

Morphology 2019, 2019

Další údaje

Jazyk

angličtina

Typ výsledku

Prezentace na konferencích

Obor

30106 Anatomy and morphology

Stát vydavatele

Slovensko

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14110/19:00110621

Organizační jednotka

Lékařská fakulta

ISBN

978-80-8152-758-6

Klíčová slova anglicky

DRG; Dorsal Root Ganglia; Paclitaxel; Taxol; NaV; NaV1.8; 1.8; Peripheral Neuropathy

Příznaky

Mezinárodní význam

Změněno: 27. 11. 2020 08:29, MUDr. Shahaf Levin

Anotace

V originále

Introduction: The chemotherapeutic agent Paclitaxel causes the adverse reaction of peripheral neuropathy. The Nav sodium channel family has an important role in pain sensation. The subtype Nav 1.8 is the more abundantly expressed Tetrodotoxin resistant (TTX-R) sodium channel expressed in nociceptive Dorsal Root Ganglia (DRG) neurons. The aim of our study was to assess possible changes in Nav 1.8 channel expression in the DRG after intraperitoneal Paclitaxel application. Material and methods: Wistar rats (n=27, males) were used in our experiments. Intraperitoneal injections of Paclitaxel in 4 doses with a cumulative dose of 8 mg/kg were performed on experimental rats, while control animals received only the excipient (alcohol and cremophor EL; 1:1). The animals were left to survive for 1, 7, 14 and 21 days from the last application. Experimental and control rats were sacrificed together with naive rats and perfused transcardially by Zambonis fixative. Longitudinal cryostat sections through the lumbar DRGs were cut and immunostained for Nav 1.8. The intensity of Nav 1.8 immunofluorescence in the DRGs was measured and statistically analyzed. Results: The presence of Nav 1.8 immunofluorescence was found in lumbar DRG neurons in naive, control and Paclitaxel treated animals. Statistically significant increase in Nav 1.8 immunofluorescence was found in the DRGs of Paclitaxel treated animals in comparison to both control and naive animals. Interestingly, positivity was also detected in vessels walls within the DRG. Conclusions: Our results indicate that DRG neurons react to Paclitaxel application by increasing Nav 1.8 channel expression. These changes might be associated with the symptoms of chemotherapy induced peripheral neuropathy.

Návaznosti

MUNI/A/1086/2018, interní kód MU

Název: Reakce struktur nervové soustavy na poškození

Investor: Masarykova univerzita, Reakce struktur nervové soustavy na poškození, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

LEVIN, Shahaf, Petr DUBOVÝ a Marek JOUKAL. Expression of sodium channel NaV 1.8 in dorsal root ganglia after Paclitaxel treatment. In Morphology 2019. 2019. ISBN 978-80-8152-758-6.

@proceedings{1554916,
   author = {Levin, Shahaf and Dubový, Petr and Joukal, Marek},
   booktitle = {Morphology 2019},
   keywords = {DRG; Dorsal Root Ganglia; Paclitaxel; Taxol; NaV; NaV1.8; 1.8; Peripheral Neuropathy},
   language = {eng},
   isbn = {978-80-8152-758-6},
   title = {Expression of sodium channel NaV 1.8 in dorsal root ganglia after Paclitaxel treatment},
   year = {2019}
}

TY  - CONF
ID  - 1554916
AU  - Levin, Shahaf - Dubový, Petr - Joukal, Marek
PY  - 2019
TI  - Expression of sodium channel NaV 1.8 in dorsal root ganglia after Paclitaxel treatment
SN  - 9788081527586
KW  - DRG
KW  - Dorsal Root Ganglia
KW  - Paclitaxel
KW  - Taxol
KW  - NaV
KW  - NaV1.8
KW  - 1.8
KW  - Peripheral Neuropathy
N2  - Introduction: The chemotherapeutic agent Paclitaxel causes the adverse reaction of peripheral neuropathy. The Nav sodium channel family has an important role in pain sensation. The subtype Nav 1.8 is the more abundantly expressed Tetrodotoxin resistant (TTX-R) sodium channel expressed in nociceptive Dorsal Root Ganglia (DRG) neurons. The aim of our study was to assess possible changes in Nav 1.8 channel expression in the DRG after intraperitoneal Paclitaxel application. Material and methods: Wistar rats (n=27, males) were used in our experiments. Intraperitoneal injections of Paclitaxel in 4 doses with a cumulative dose of 8 mg/kg were performed on experimental rats, while control animals received only the excipient (alcohol and cremophor EL; 1:1). The animals were left to survive for 1, 7, 14 and 21 days from the last application. Experimental and control rats were sacrificed together with naive rats and perfused transcardially by Zambonis fixative. Longitudinal cryostat sections through the lumbar DRGs were cut and immunostained for Nav 1.8. The intensity of Nav 1.8 immunofluorescence in the DRGs was measured and statistically analyzed. Results: The presence of Nav 1.8 immunofluorescence was found in lumbar DRG neurons in naive, control and Paclitaxel treated animals. Statistically significant increase in Nav 1.8 immunofluorescence was found in the DRGs of Paclitaxel treated animals in comparison to both control and naive animals. Interestingly, positivity was also detected in vessels walls within the DRG. Conclusions: Our results indicate that DRG neurons react to Paclitaxel application by increasing Nav 1.8 channel expression. These changes might be associated with the symptoms of chemotherapy induced peripheral neuropathy.
ER  -

LEVIN, Shahaf, Petr DUBOVÝ a Marek JOUKAL. Expression of sodium channel NaV 1.8 in dorsal root ganglia after Paclitaxel treatment. In \textit{Morphology 2019}. 2019. ISBN~978-80-8152-758-6.

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