Motility and cytoskeletal organisation in the archigregarine
Selenidium pygospionis (Apicomplexa): observations on native
and experimentally affected parasites

J 2019

Motility and cytoskeletal organisation in the archigregarine Selenidium pygospionis (Apicomplexa): observations on native and experimentally affected parasites

KOVÁČIKOVÁ, Magdaléna, Gita G. PASKEROVA, Andrei DIAKIN, Timur G. SIMDYANOV, Naděžda VAŠKOVICOVÁ et. al.

Základní údaje

Originální název

Motility and cytoskeletal organisation in the archigregarine Selenidium pygospionis (Apicomplexa): observations on native and experimentally affected parasites

Autoři

KOVÁČIKOVÁ, Magdaléna (703 Slovensko, garant, domácí), Gita G. PASKEROVA (643 Rusko), Andrei DIAKIN (643 Rusko, domácí), Timur G. SIMDYANOV (643 Rusko), Naděžda VAŠKOVICOVÁ (203 Česká republika) a Andrea BARDŮNEK VALIGUROVÁ (703 Slovensko, domácí)

Vydání

Parasitology research, New York, Springer, 2019, 0932-0113

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.641

Kód RIV

RIV/00216224:14310/19:00107631

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1007/s00436-019-06381-z

UT WoS

000482439500020

Klíčová slova anglicky

Actin; Cytoskeletal drugs; Microtubules; Motility; Ultrastructure; alpha-Tubulin

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 3. 2020 16:51, Mgr. Marie Šípková, DiS.

Anotace

V originále

Representatives of Apicomplexa perform various kinds of movements that are linked to the different stages of their life cycle. Ancestral apicomplexan lineages, including gregarines, represent organisms suitable for research into the evolution and diversification of motility within the group. The vermiform trophozoites and gamonts of the archigregarine Selenidium pygospionis perform a very active type of bending motility. Experimental assays and subsequent light, electron, and confocal microscopic analyses demonstrated the fundamental role of the cytoskeletal proteins actin and tubulin in S. pygospionis motility and allowed us to compare the mechanism of its movement to the gliding machinery (the so-called glideosome concept) described in apicomplexan zoites. Actin-modifying drugs caused a reduction in the movement speed (cytochalasin D) or stopped the motility of archigregarines completely (jasplakinolide). Microtubule-disrupting drugs (oryzalin and colchicine) had an even more noticeable effect on archigregarine motility. The fading and disappearance of microtubules were documented in ultrathin sections, along with the formation of alpha-tubulin clusters visible after the immunofluorescent labelling of drug-treated archigregarines. The obtained data indicate that subpellicular microtubules most likely constitute the main motor structure involved in S. pygospionis bending motility, while actin has rather a supportive function.

Návaznosti

GBP505/12/G112, projekt VaV

Název: ECIP - Evropské centrum ichtyoparazitologie

Investor: Grantová agentura ČR, ECIP - Evropské centrum ichtyoparazitologie

Citovat

KOVÁČIKOVÁ, Magdaléna, Gita G. PASKEROVA, Andrei DIAKIN, Timur G. SIMDYANOV, Naděžda VAŠKOVICOVÁ a Andrea BARDŮNEK VALIGUROVÁ. Motility and cytoskeletal organisation in the archigregarine Selenidium pygospionis (Apicomplexa): observations on native and experimentally affected parasites. Parasitology research. New York: Springer, 2019, roč. 118, č. 9, s. 2651-2667. ISSN 0932-0113. Dostupné z: https://dx.doi.org/10.1007/s00436-019-06381-z.

@article{1556898,
   author = {Kováčiková, Magdaléna and Paskerova, Gita G. and Diakin, Andrei and Simdyanov, Timur G. and Vaškovicová, Naděžda and Bardůnek Valigurová, Andrea},
   article_location = {New York},
   article_number = {9},
   doi = {http://dx.doi.org/10.1007/s00436-019-06381-z},
   keywords = {Actin; Cytoskeletal drugs; Microtubules; Motility; Ultrastructure; alpha-Tubulin},
   language = {eng},
   issn = {0932-0113},
   journal = {Parasitology research},
   title = {Motility and cytoskeletal organisation in the archigregarine Selenidium pygospionis (Apicomplexa): observations on native and experimentally affected parasites},
   url = {http://link.springer.com/article/10.1007/s00436-019-06381-z},
   volume = {118},
   year = {2019}
}

TY  - JOUR
ID  - 1556898
AU  - Kováčiková, Magdaléna - Paskerova, Gita G. - Diakin, Andrei - Simdyanov, Timur G. - Vaškovicová, Naděžda - Bardůnek Valigurová, Andrea
PY  - 2019
TI  - Motility and cytoskeletal organisation in the archigregarine Selenidium pygospionis (Apicomplexa): observations on native and experimentally affected parasites
JF  - Parasitology research
VL  - 118
IS  - 9
SP  - 2651-2667
EP  - 2651-2667
PB  - Springer
SN  - 09320113
KW  - Actin
KW  - Cytoskeletal drugs
KW  - Microtubules
KW  - Motility
KW  - Ultrastructure
KW  - alpha-Tubulin
UR  - http://link.springer.com/article/10.1007/s00436-019-06381-z
L2  - http://link.springer.com/article/10.1007/s00436-019-06381-z
N2  - Representatives of Apicomplexa perform various kinds of movements that are linked to the different stages of their life cycle. Ancestral apicomplexan lineages, including gregarines, represent organisms suitable for research into the evolution and diversification of motility within the group. The vermiform trophozoites and gamonts of the archigregarine Selenidium pygospionis perform a very active type of bending motility. Experimental assays and subsequent light, electron, and confocal microscopic analyses demonstrated the fundamental role of the cytoskeletal proteins actin and tubulin in S. pygospionis motility and allowed us to compare the mechanism of its movement to the gliding machinery (the so-called glideosome concept) described in apicomplexan zoites. Actin-modifying drugs caused a reduction in the movement speed (cytochalasin D) or stopped the motility of archigregarines completely (jasplakinolide). Microtubule-disrupting drugs (oryzalin and colchicine) had an even more noticeable effect on archigregarine motility. The fading and disappearance of microtubules were documented in ultrathin sections, along with the formation of alpha-tubulin clusters visible after the immunofluorescent labelling of drug-treated archigregarines. The obtained data indicate that subpellicular microtubules most likely constitute the main motor structure involved in S. pygospionis bending motility, while actin has rather a supportive function.
ER  -

KOVÁČIKOVÁ, Magdaléna, Gita G. PASKEROVA, Andrei DIAKIN, Timur G. SIMDYANOV, Naděžda VAŠKOVICOVÁ a Andrea BARDŮNEK VALIGUROVÁ. Motility and cytoskeletal organisation in the archigregarine Selenidium pygospionis (Apicomplexa): observations on native and experimentally affected parasites. \textit{Parasitology research}. New York: Springer, 2019, roč.~118, č.~9, s.~2651-2667. ISSN~0932-0113. Dostupné z: https://dx.doi.org/10.1007/s00436-019-06381-z.

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