| FEDOROVÁ, Veronika, Tereza VÁŇOVÁ, Lina Mohamed Bahaael ELREFAE, Jakub POSPÍŠIL, Martina PETRÁŠOVÁ, Veronika KOLAJOVÁ, Zuzana HUDACOVA, Jana BANIARIOVÁ, Martin BARÁK, Lucie PEŠKOVÁ, Tomáš BÁRTA, Markéta KAUCKÁ, Michael KILLINGER, Josef VEČEŘA, Ondřej BERNATÍK, Lukáš ČAJÁNEK, Hana HŘÍBKOVÁ and Dáša BOHAČIAKOVÁ. Differentiation of neural rosettes from human pluripotent stem cells in vitro is sequentially regulated on a molecular level and accomplished by the mechanism reminiscent of secondary neurulation. Stem Cell Research. Amsterdam: Elsevier Science BV, 2019, vol. 40, OCT 2019, p. 1-10. ISSN 1873-5061. Available from: https://dx.doi.org/10.1016/j.scr.2019.101563. |
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@article{1559896,
author = {Fedorová, Veronika and Váňová, Tereza and Elrefae, Lina Mohamed Bahaael and Pospíšil, Jakub and Petrášová, Martina and Kolajová, Veronika and Hudacova, Zuzana and Baniariová, Jana and Barák, Martin and Pešková, Lucie and Bárta, Tomáš and Kaucká, Markéta and Killinger, Michael and Večeřa, Josef and Bernatík, Ondřej and Čajánek, Lukáš and Hříbková, Hana and Bohačiaková, Dáša},
article_location = {Amsterdam},
article_number = {OCT 2019},
doi = {http://dx.doi.org/10.1016/j.scr.2019.101563},
keywords = {BMP; Differentiation; Human embryonic stem cells; Induced pluripotent stem cells; Neural rosettes; Secondary neurulation},
language = {eng},
issn = {1873-5061},
journal = {Stem Cell Research},
title = {Differentiation of neural rosettes from human pluripotent stem cells in vitro is sequentially regulated on a molecular level and accomplished by the mechanism reminiscent of secondary neurulation},
url = {https://www.sciencedirect.com/science/article/pii/S187350611930193X?via%3Dihub},
volume = {40},
year = {2019}
}
TY - JOUR
ID - 1559896
AU - Fedorová, Veronika - Váňová, Tereza - Elrefae, Lina Mohamed Bahaael - Pospíšil, Jakub - Petrášová, Martina - Kolajová, Veronika - Hudacova, Zuzana - Baniariová, Jana - Barák, Martin - Pešková, Lucie - Bárta, Tomáš - Kaucká, Markéta - Killinger, Michael - Večeřa, Josef - Bernatík, Ondřej - Čajánek, Lukáš - Hříbková, Hana - Bohačiaková, Dáša
PY - 2019
TI - Differentiation of neural rosettes from human pluripotent stem cells in vitro is sequentially regulated on a molecular level and accomplished by the mechanism reminiscent of secondary neurulation
JF - Stem Cell Research
VL - 40
IS - OCT 2019
SP - 1-10
EP - 1-10
PB - Elsevier Science BV
SN - 18735061
KW - BMP
KW - Differentiation
KW - Human embryonic stem cells
KW - Induced pluripotent stem cells
KW - Neural rosettes
KW - Secondary neurulation
UR - https://www.sciencedirect.com/science/article/pii/S187350611930193X?via%3Dihub
L2 - https://www.sciencedirect.com/science/article/pii/S187350611930193X?via%3Dihub
N2 - Development of neural tube has been extensively modeled in vitro using human pluripotent stem cells (hPSCs) that are able to form radially organized cellular structures called neural rosettes. While a great amount of research has been done using neural rosettes, studies have only inadequately addressed how rosettes are formed and what the molecular mechanisms and pathways involved in their formation are. Here we address this question by detailed analysis of the expression of pluripotency and differentiation-associated proteins during the early onset of differentiation of hPSCs towards neural rosettes. Additionally, we show that the BMP signaling is likely contributing to the formation of the complex cluster of neural rosettes and its inhibition leads to the altered expression of PAX6, SOX2 and SOX1 proteins and the rosette morphology. Finally, we provide evidence that the mechanism of neural rosettes formation in vitro is reminiscent of the process of secondary neurulation rather than that of primary neurulation in vivo. Since secondary neurulation is a largely unexplored process, its understanding will ultimately assist the development of methods to prevent caudal neural tube defects in humans.
ER -
FEDOROVÁ, Veronika, Tereza VÁŇOVÁ, Lina Mohamed Bahaael ELREFAE, Jakub POSPÍŠIL, Martina PETRÁŠOVÁ, Veronika KOLAJOVÁ, Zuzana HUDACOVA, Jana BANIARIOVÁ, Martin BARÁK, Lucie PEŠKOVÁ, Tomáš BÁRTA, Markéta KAUCKÁ, Michael KILLINGER, Josef VEČEŘA, Ondřej BERNATÍK, Lukáš ČAJÁNEK, Hana HŘÍBKOVÁ and Dáša BOHAČIAKOVÁ. Differentiation of neural rosettes from human pluripotent stem cells in vitro is sequentially regulated on a molecular level and accomplished by the mechanism reminiscent of secondary neurulation. \textit{Stem Cell Research}. Amsterdam: Elsevier Science BV, 2019, vol.~40, OCT 2019, p.~1-10. ISSN~1873-5061. Available from: https://dx.doi.org/10.1016/j.scr.2019.101563.
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