Další formáty:
BibTeX
LaTeX
RIS
@article{1561176,
author = {Schořová, Šárka and Fajkus, Jiří and Záveská Drábková, Lenka and Honys, David and Procházková Schrumpfová, Petra},
article_number = {2},
doi = {http://dx.doi.org/10.1111/tpj.14306},
keywords = {Telomerase assembly; Pontin; Reptin; AtTERT; AtTRB; AtRuvBL; AtPOT1a; nucleolus; Arabidopsis.},
language = {eng},
issn = {0960-7412},
journal = {The Plant Journal},
title = {The plant Pontin and Reptin homologues, RuvBL1 and RuvBL2a, colocalize with TERT and TRB proteins in vivo, and participate in telomerase biogenesis},
url = {https://onlinelibrary.wiley.com/doi/10.1111/tpj.14306},
volume = {98},
year = {2019}
}
TY - JOUR
ID - 1561176
AU - Schořová, Šárka - Fajkus, Jiří - Záveská Drábková, Lenka - Honys, David - Procházková Schrumpfová, Petra
PY - 2019
TI - The plant Pontin and Reptin homologues, RuvBL1 and RuvBL2a, colocalize with TERT and TRB proteins in vivo, and participate in telomerase biogenesis
JF - The Plant Journal
VL - 98
IS - 2
SP - 195-212
EP - 195-212
PB - Blackwell Science
SN - 09607412
KW - Telomerase assembly
KW - Pontin
KW - Reptin
KW - AtTERT
KW - AtTRB
KW - AtRuvBL
KW - AtPOT1a
KW - nucleolus
KW - Arabidopsis.
UR - https://onlinelibrary.wiley.com/doi/10.1111/tpj.14306
L2 - https://onlinelibrary.wiley.com/doi/10.1111/tpj.14306
N2 - Telomerase maturation and recruitment to telomeres is regulated by several telomerase- and telomereassociated proteins. Among a number of proteins, human Pontin and Reptin play critical roles in telomerase biogenesis. Here we characterized plant orthologues of Pontin and Reptin, RuvBL1 and RuvBL2a, respectively, and show association of Arabidopsis thaliana RuvBL1 (AtRuvBL1) with the catalytic subunit of telomerase (AtTERT) in the nucleolus in vivo. In contrast to mammals, interactions between AtTERT and AtRuvBL proteins in A. thaliana are not direct and they are rather mediated by one of the Arabidopsis thaliana Telomere Repeat Binding (AtTRB) proteins. We further show that plant orthologue of dyskerin, named AtCBF5, is indirectly associated with AtTRB proteins but not with the AtRuvBL proteins in the plant nucleus/nucleolus, and interacts with the Protection of telomere 1 (AtPOT1a) in the nucleolus or cytoplasmic foci. Our genomewide phylogenetic analyses identify orthologues in RuvBL protein family within the plant kingdom. Dysfunction of AtRuvBL genes in heterozygous T-DNA insertion A. thaliana mutants results in reduced telomerase activity and indicate the involvement of AtRuvBL in plant telomerase biogenesis.
ER -
SCHOŘOVÁ, Šárka, Jiří FAJKUS, Lenka ZÁVESKÁ DRÁBKOVÁ, David HONYS a Petra PROCHÁZKOVÁ SCHRUMPFOVÁ. The plant Pontin and Reptin homologues, RuvBL1 and RuvBL2a, colocalize with TERT and TRB proteins in vivo, and participate in telomerase biogenesis. \textit{The Plant Journal}. Blackwell Science, 2019, roč.~98, č.~2, s.~195-212. ISSN~0960-7412. Dostupné z: https://dx.doi.org/10.1111/tpj.14306.
|
