The region upstream of the telomerase reverse transcriptase
gene is essential for in planta telomerase complementation

J 2019

The region upstream of the telomerase reverse transcriptase gene is essential for in planta telomerase complementation

CRHÁK, Tomáš, Dagmar ZACHOVÁ, Miloslava FOJTOVÁ a Eva SÝKOROVÁ

Základní údaje

Originální název

The region upstream of the telomerase reverse transcriptase gene is essential for in planta telomerase complementation

Autoři

CRHÁK, Tomáš (203 Česká republika, domácí), Dagmar ZACHOVÁ (203 Česká republika, domácí), Miloslava FOJTOVÁ (203 Česká republika, domácí) a Eva SÝKOROVÁ (203 Česká republika, garant)

Vydání

Plant Science, Elsevier Ireland Ltd. 2019, 0168-9452

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

odkaz na plný text článku

Impakt faktor

Impact factor: 3.591

Kód RIV

RIV/00216224:14740/19:00107676

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1016/j.plantsci.2019.01.001

UT WoS

000461265000005

Klíčová slova anglicky

Arabidopsis thaliana; complementation; in planta; promoter; telomerase; telomere

Štítky

CF PLANT, in planta, komplementace, promotor, rivok, telomeráza

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 4. 3. 2020 14:45, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Telomerase is essential for the maintenance of telomeres, structures located at the ends of linear eukaryotic chromosomes that are crucial for genomic stability. Telomerase has been frequently explored in mammals because of its activity in many types of cancers, but knowledge in plants is rather sketchy despite plants representing useful models due to peculiarities in their telomeres and telomerase biology. We studied in planta complementation of telomerase in Arabidopsis thaliana mutant plants with disrupted expression of the gene encoding the telomerase protein subunit (AtTERT) and significantly shortened telomeres. We found that the upstream region of AtTERT, previously identified as a putative minimal promoter, was essential for reconstitution of telomerase function, as demonstrated by the full or partial recovery of the telomere phenotype in mutants. In contrast, transformation by the full length AtTERT gene construct resulted in more progressive telomere shortening in mutants and even in wild type plants, despite the high level of AtTERT transcript and telomerase activity detected by in vitro assay. Thus, the telomerase protein subunit putative promoter is essential for in planta telomerase reconstitution and restoration of its catalytical activity. Contributions from other factors, including those tissue-specific, for proper telomerase function are discussed.

Návaznosti

GA18-07027S, projekt VaV

Název: Zapojení telomerázy do buněčného interaktomu

Investor: Grantová agentura ČR, Zapojení telomerázy do buněčného interaktomu

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

CRHÁK, Tomáš, Dagmar ZACHOVÁ, Miloslava FOJTOVÁ a Eva SÝKOROVÁ. The region upstream of the telomerase reverse transcriptase gene is essential for in planta telomerase complementation. Plant Science. Elsevier Ireland Ltd., 2019, roč. 281, č. 281, s. 41-51. ISSN 0168-9452. Dostupné z: https://dx.doi.org/10.1016/j.plantsci.2019.01.001.

@article{1561458,
   author = {Crhák, Tomáš and Zachová, Dagmar and Fojtová, Miloslava and Sýkorová, Eva},
   article_number = {281},
   doi = {http://dx.doi.org/10.1016/j.plantsci.2019.01.001},
   keywords = {Arabidopsis thaliana; complementation; in planta; promoter; telomerase; telomere},
   language = {eng},
   issn = {0168-9452},
   journal = {Plant Science},
   title = {The region upstream of the telomerase reverse transcriptase gene is essential for in planta telomerase complementation},
   url = {https://www.sciencedirect.com/science/article/pii/S0168945218314511?via%3Dihub},
   volume = {281},
   year = {2019}
}

TY  - JOUR
ID  - 1561458
AU  - Crhák, Tomáš - Zachová, Dagmar - Fojtová, Miloslava - Sýkorová, Eva
PY  - 2019
TI  - The region upstream of the telomerase reverse transcriptase gene is essential for in planta telomerase complementation
JF  - Plant Science
VL  - 281
IS  - 281
SP  - 41-51
EP  - 41-51
PB  - Elsevier Ireland Ltd.
SN  - 01689452
KW  - Arabidopsis thaliana
KW  - complementation
KW  - in planta
KW  - promoter
KW  - telomerase
KW  - telomere
UR  - https://www.sciencedirect.com/science/article/pii/S0168945218314511?via%3Dihub
L2  - https://www.sciencedirect.com/science/article/pii/S0168945218314511?via%3Dihub
N2  - Telomerase is essential for the maintenance of telomeres, structures located at the ends of linear eukaryotic chromosomes that are crucial for genomic stability. Telomerase has been frequently explored in mammals because of its activity in many types of cancers, but knowledge in plants is rather sketchy despite plants representing useful models due to peculiarities in their telomeres and telomerase biology. We studied in planta complementation of telomerase in Arabidopsis thaliana mutant plants with disrupted expression of the gene encoding the telomerase protein subunit (AtTERT) and significantly shortened telomeres. We found that the upstream region of AtTERT, previously identified as a putative minimal promoter, was essential for reconstitution of telomerase function, as demonstrated by the full or partial recovery of the telomere phenotype in mutants. In contrast, transformation by the full length AtTERT gene construct resulted in more progressive telomere shortening in mutants and even in wild type plants, despite the high level of AtTERT transcript and telomerase activity detected by in vitro assay. Thus, the telomerase protein subunit putative promoter is essential for in planta telomerase reconstitution and restoration of its catalytical activity. Contributions from other factors, including those tissue-specific, for proper telomerase function are discussed.
ER  -

CRHÁK, Tomáš, Dagmar ZACHOVÁ, Miloslava FOJTOVÁ a Eva SÝKOROVÁ. The region upstream of the telomerase reverse transcriptase gene is essential for in planta telomerase complementation. \textit{Plant Science}. Elsevier Ireland Ltd., 2019, roč.~281, č.~281, s.~41-51. ISSN~0168-9452. Dostupné z: https://dx.doi.org/10.1016/j.plantsci.2019.01.001.

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