The molecular and morphometric identification of Dictyocaulus
capreolus in clinically affected roe deer (Capreolus capreolus
L.)

J 2019

The molecular and morphometric identification of Dictyocaulus capreolus in clinically affected roe deer (Capreolus capreolus L.)

JURÁNKOVÁ, Jana; Dagmar JIRSOVÁ; Barbora KALOUSOVÁ and Pavel FOREJTEK

Basic information

Original name

The molecular and morphometric identification of Dictyocaulus capreolus in clinically affected roe deer (Capreolus capreolus L.)

Authors

JURÁNKOVÁ, Jana (203 Czech Republic, guarantor); Dagmar JIRSOVÁ (203 Czech Republic, belonging to the institution); Barbora KALOUSOVÁ (203 Czech Republic) and Pavel FOREJTEK

Edition

Veterinarni Medicina, Praha, CZECH ACADEMY AGRICULTURAL SCIENCES, 2019, 0375-8427

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

10613 Zoology

Country of publisher

Czech Republic

Confidentiality degree

is not subject to a state or trade secret

References:

Full Text

Impact factor

Impact factor: 0.588

RIV identification code

RIV/00216224:14310/19:00110795

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.17221/9/2019-VETMED

UT WoS

000488083000002

EID Scopus

2-s2.0-85072937462

Keywords in English

lung nematodes; rRNA; respiratory tract; anorexia; diarrhoea

Abstract

In the original language

The poor state of health and increased mortality rate of young roe deer, as reported by South Moravian hunters, caused by the increasing numbers of adult nematodes in the lungs of roe deer prompted us to identify the parasites using a combination of morphological measurements and a phylogenetic SSU rRNA analysis. The study was conducted in a 294 ha game reserve in South Moravia, Czech Republic. Molecular and morphometric techniques were used to identify adult nematodes collected from the respiratory tracts of nine 4–5 months old roe deer in poor health (low body weight of 3–4 kg, poor haircoat quality, and, in some cases, symptoms of diarrhoea). The morphological identification was based on a combination of adult worm characteristics corresponding to Dictyocaulus capreolus. A small subunit rRNA (SSU) partial sequence analysis showed the highest identity scores (99%) corresponding to the sequences of D. capreolus from a roe deer (GenBank: AY168859) from Sweden and the outcomes of the phylogenetic analyses resulted in a tree with a high branch support for two groups, with our sequences forming a well-supported clade with D. capreolus and Dictyocaulus sp. ex Capreolus capreolus (FJ589016) and Dictyocaulus sp. ex Rupicapra rupicapra (FJ589019) sequences from Spain. The examined roe deer have shown symptoms of diarrhoea, anorexia, and respiratory tract inflammation indicating that there might be a connection to the clinical importance of the Dictyocaulus infection

Links

LM2015085, research and development project

Name: CERIT Scientific Cloud (Acronym: CERIT-SC)

Investor: Ministry of Education, Youth and Sports of the CR, CERIT Scientific Cloud

Cite

JURÁNKOVÁ, Jana; Dagmar JIRSOVÁ; Barbora KALOUSOVÁ and Pavel FOREJTEK. The molecular and morphometric identification of Dictyocaulus capreolus in clinically affected roe deer (Capreolus capreolus L.). Veterinarni Medicina. Praha: CZECH ACADEMY AGRICULTURAL SCIENCES, 2019, vol. 64, No 9, p. 386-391. ISSN 0375-8427. Available from: https://dx.doi.org/10.17221/9/2019-VETMED.

@article{1563039,
   author = {Juránková, Jana and Jirsová, Dagmar and Kalousová, Barbora and Forejtek, Pavel},
   article_location = {Praha},
   article_number = {9},
   doi = {http://dx.doi.org/10.17221/9/2019-VETMED},
   keywords = {lung nematodes; rRNA; respiratory tract; anorexia; diarrhoea},
   language = {eng},
   issn = {0375-8427},
   journal = {Veterinarni Medicina},
   title = {The molecular and morphometric identification of Dictyocaulus capreolus in clinically affected roe deer (Capreolus capreolus L.)},
   url = {https://www.agriculturejournals.cz/web/vetmed.htm?type=article&id=9_2019-VETMED},
   volume = {64},
   year = {2019}
}

TY  - JOUR
ID  - 1563039
AU  - Juránková, Jana - Jirsová, Dagmar - Kalousová, Barbora - Forejtek, Pavel
PY  - 2019
TI  - The molecular and morphometric identification of Dictyocaulus capreolus in clinically affected roe deer (Capreolus capreolus L.)
JF  - Veterinarni Medicina
VL  - 64
IS  - 9
SP  - 386-391
EP  - 386-391
PB  - CZECH ACADEMY AGRICULTURAL SCIENCES
SN  - 03758427
KW  - lung nematodes
KW  - rRNA
KW  - respiratory tract
KW  - anorexia
KW  - diarrhoea
UR  - https://www.agriculturejournals.cz/web/vetmed.htm?type=article&id=9_2019-VETMED
L2  - https://www.agriculturejournals.cz/web/vetmed.htm?type=article&id=9_2019-VETMED
N2  - The poor state of health and increased mortality rate of young roe deer, as reported by South Moravian hunters, caused by the increasing numbers of adult nematodes in the lungs of roe deer prompted us to identify the parasites using a combination of morphological measurements and a phylogenetic SSU rRNA analysis. The study was conducted in a 294 ha game reserve in South Moravia, Czech Republic. Molecular and morphometric techniques were used to identify adult nematodes collected from the respiratory tracts of nine 4–5 months old roe deer in poor health (low body weight of 3–4 kg, poor haircoat quality, and, in some cases, symptoms of diarrhoea). The morphological identification was based on a combination of adult worm characteristics corresponding to Dictyocaulus capreolus. A small subunit rRNA (SSU) partial sequence analysis showed the highest identity scores (99%) corresponding to the sequences of D. capreolus from a roe deer (GenBank: AY168859) from Sweden and the outcomes of the phylogenetic analyses resulted in a tree with a high branch support for two groups, with our sequences forming a well-supported clade with D. capreolus and Dictyocaulus sp. ex Capreolus capreolus (FJ589016) and Dictyocaulus sp. ex Rupicapra rupicapra (FJ589019) sequences from Spain. The examined roe deer have shown symptoms of diarrhoea, anorexia, and respiratory tract inflammation indicating that there might be a connection to the clinical importance of the Dictyocaulus infection
ER  -

JURÁNKOVÁ, Jana; Dagmar JIRSOVÁ; Barbora KALOUSOVÁ and Pavel FOREJTEK. The molecular and morphometric identification of Dictyocaulus capreolus in clinically affected roe deer (Capreolus capreolus L.). \textit{Veterinarni Medicina}. Praha: CZECH ACADEMY AGRICULTURAL SCIENCES, 2019, vol.~64, No~9, p.~386-391. ISSN~0375-8427. Available from: https://dx.doi.org/10.17221/9/2019-VETMED.

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