Detailed Information on Publication Record
2017
Phosphorylation-Dependent Feedback Inhibition of RIG-I by DAPK1 Identified by Kinome-wide siRNA Screening
WILLEMSEN, Joschka, Oliver WICHT, Julia WOLANSKI, Nina BAUR, Sandra BASTIAN et. al.Basic information
Original name
Phosphorylation-Dependent Feedback Inhibition of RIG-I by DAPK1 Identified by Kinome-wide siRNA Screening
Authors
WILLEMSEN, Joschka, Oliver WICHT, Julia WOLANSKI, Nina BAUR, Sandra BASTIAN, Darya HAAS, Petr MATULA (203 Czech Republic, guarantor, belonging to the institution), Bettina KNAPP, Laurene MEYNIEL-SCHICKLIN, Chen WANG, Ralf BARTENSCHLAGER, Volker LOHMANN, Karl ROHR, Holger ERFLE, Lars KADERALI, Joseph MARCOTRIGIANO, Andreas PICHLMAIR and Marco BINDER
Edition
Molecular Cell, CAMBRIDGE, CELL PRESS, 2017, 1097-2765
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10601 Cell biology
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 14.248
RIV identification code
RIV/00216224:14330/17:00107683
Organization unit
Faculty of Informatics
UT WoS
000396431400007
Keywords in English
innate immunity; antiviral response; pattern recognition receptors; signal transduction; feedback regulation; interferon system; cytokines; DAPK1; RIG-I; DDX58
Tags
International impact, Reviewed
Změněno: 28/4/2020 07:48, RNDr. Pavel Šmerk, Ph.D.
Abstract
V originále
Cell-autonomous induction of type I interferon must be stringently regulated. Rapid induction is key to control virus infection, whereas proper limitation of signaling is essential to prevent immunopathology and autoimmune disease. Using unbiased kinome-wide RNAi screening followed by thorough validation, we identified 22 factors that regulate RIG-I/IRF3 signaling activity. We describe a negative-feedback mechanism targeting RIG-I activity, which is mediated by death associated protein kinase 1 (DAPK1). RIG-I signaling triggers DAPK1 kinase activation, and active DAPK1 potently inhibits RIG-I stimulated IRF3 activity and interferon-beta production. DAPK1 phosphorylates RIG-I in vitro at previously reported as well as other sites that limit 5'ppp-dsRNA sensing and virtually abrogate RIG-I activation.
Links
GBP302/12/G157, research and development project |
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