Optimization of background electrolyte composition for
simultaneous contactless conductivity and fluorescence
detection in capillary electrophoresis of biological samples

J 2019

Optimization of background electrolyte composition for simultaneous contactless conductivity and fluorescence detection in capillary electrophoresis of biological samples

LAČNÁ, Júlia; Jan PŘIKRYL; Norio TESHIMA; Hiroya MURAKAMI; Yukihiro ESAKA et. al.

Basic information

Original name

Optimization of background electrolyte composition for simultaneous contactless conductivity and fluorescence detection in capillary electrophoresis of biological samples

Authors

LAČNÁ, Júlia (703 Slovakia, belonging to the institution); Jan PŘIKRYL (203 Czech Republic); Norio TESHIMA; Hiroya MURAKAMI; Yukihiro ESAKA; František FORET (203 Czech Republic, belonging to the institution) and Petr KUBÁŇ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Electrophoresis, HOBOKEN, WILEY-BLACKWELL, 2019, 0173-0835

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

10406 Analytical chemistry

Country of publisher

Netherlands

Confidentiality degree

is not subject to a state or trade secret

References:

URL

Impact factor

Impact factor: 3.081

RIV identification code

RIV/00216224:14740/19:00108517

Organization unit

Central European Institute of Technology

DOI

https://doi.org/10.1002/elps.201900112

UT WoS

000483858400018

EID Scopus

2-s2.0-85068158096

Keywords in English

Capillary electrophoresis; Contactless conductivity detection; Dual detection; Laser induced fluorescence detection; Saliva

Abstract

In the original language

In this article, optimization of BGE for simultaneous separation of inorganic ions, organic acids, and glutathione using dual (CD)-D-4-LIF detection in capillary electrophoresis is presented. The optimized BGE consisted of 30 mM 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid, 15 mM 2-amino-2-hydroxymethyl-propane-1,3-diol, and 2 mM 18-crown-6 at pH 7.2 and allowed simultaneous separation of ten inorganic anions and cations, three organic acids and glutathione in 20 min. The samples were injected hydrodynamically from both capillary ends using the double-opposite end injection principle. Sensitive detection of anions, cations, and organic acids with micromolar LODs using (CD)-D-4 and simultaneously glutathione with nanomolar LODs using LIF was achieved in a single run. The developed BGE may be useful in analyses of biological samples containing analytes with differing concentrations of several orders of magnitude that is not possible with single detection mode.

Links

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

NV17-31945A, research and development project

Name: Vývoj neinvazivní diagnostiky extraezofageálních projevů refluxní choroby jícnu

Cite

LAČNÁ, Júlia; Jan PŘIKRYL; Norio TESHIMA; Hiroya MURAKAMI; Yukihiro ESAKA; František FORET and Petr KUBÁŇ. Optimization of background electrolyte composition for simultaneous contactless conductivity and fluorescence detection in capillary electrophoresis of biological samples. Electrophoresis. HOBOKEN: WILEY-BLACKWELL, 2019, vol. 40, 18-19, p. 2390-2397. ISSN 0173-0835. Available from: https://doi.org/10.1002/elps.201900112.

@article{1566837,
   author = {Lačná, Júlia and Přikryl, Jan and Teshima, Norio and Murakami, Hiroya and Esaka, Yukihiro and Foret, František and Kubáň, Petr},
   article_location = {HOBOKEN},
   article_number = {18-19},
   doi = {https://doi.org/10.1002/elps.201900112},
   keywords = {Capillary electrophoresis; Contactless conductivity detection; Dual detection; Laser induced fluorescence detection; Saliva},
   language = {eng},
   issn = {0173-0835},
   journal = {Electrophoresis},
   title = {Optimization of background electrolyte composition for simultaneous contactless conductivity and fluorescence detection in capillary electrophoresis of biological samples},
   url = {http://dx.doi.org/10.1002/elps.201900112},
   volume = {40},
   year = {2019}
}

TY  - JOUR
ID  - 1566837
AU  - Lačná, Júlia - Přikryl, Jan - Teshima, Norio - Murakami, Hiroya - Esaka, Yukihiro - Foret, František - Kubáň, Petr
PY  - 2019
TI  - Optimization of background electrolyte composition for simultaneous contactless conductivity and fluorescence detection in capillary electrophoresis of biological samples
JF  - Electrophoresis
VL  - 40
IS  - 18-19
SP  - 2390-2397
EP  - 2390-2397
PB  - WILEY-BLACKWELL
SN  - 01730835
KW  - Capillary electrophoresis
KW  - Contactless conductivity detection
KW  - Dual detection
KW  - Laser induced fluorescence detection
KW  - Saliva
UR  - http://dx.doi.org/10.1002/elps.201900112
L2  - http://dx.doi.org/10.1002/elps.201900112
N2  - In this article, optimization of BGE for simultaneous separation of inorganic ions, organic acids, and glutathione using dual (CD)-D-4-LIF detection in capillary electrophoresis is presented. The optimized BGE consisted of 30 mM 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid, 15 mM 2-amino-2-hydroxymethyl-propane-1,3-diol, and 2 mM 18-crown-6 at pH 7.2 and allowed simultaneous separation of ten inorganic anions and cations, three organic acids and glutathione in 20 min. The samples were injected hydrodynamically from both capillary ends using the double-opposite end injection principle. Sensitive detection of anions, cations, and organic acids with micromolar LODs using (CD)-D-4 and simultaneously glutathione with nanomolar LODs using LIF was achieved in a single run. The developed BGE may be useful in analyses of biological samples containing analytes with differing concentrations of several orders of magnitude that is not possible with single detection mode.
ER  -

LAČNÁ, Júlia; Jan PŘIKRYL; Norio TESHIMA; Hiroya MURAKAMI; Yukihiro ESAKA; František FORET and Petr KUBÁŇ. Optimization of background electrolyte composition for simultaneous contactless conductivity and fluorescence detection in capillary electrophoresis of biological samples. \textit{Electrophoresis}. HOBOKEN: WILEY-BLACKWELL, 2019, vol.~40, 18-19, p.~2390-2397. ISSN~0173-0835. Available from: https://doi.org/10.1002/elps.201900112.

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