The Effect of Uncoated SPIONs on hiPSC-Differentiated
Endothelial Cells

J 2019

The Effect of Uncoated SPIONs on hiPSC-Differentiated Endothelial Cells

ŠALINGOVÁ, Barbara, Pavel ŠIMARA, Pavel MATULA, Lenka ZAJÍČKOVÁ, Petr SYNEK et. al.

Základní údaje

Originální název

The Effect of Uncoated SPIONs on hiPSC-Differentiated Endothelial Cells

Autoři

ŠALINGOVÁ, Barbara (203 Česká republika, domácí), Pavel ŠIMARA (203 Česká republika, domácí), Pavel MATULA (203 Česká republika, domácí), Lenka ZAJÍČKOVÁ (203 Česká republika, domácí), Petr SYNEK (203 Česká republika, domácí), Ondřej JAŠEK (203 Česká republika, domácí), Lenka VEVERKOVÁ (203 Česká republika, domácí), Miroslava SEDLÁČKOVÁ (203 Česká republika, domácí), Zuzana NICHTOVA (703 Slovensko) a Irena KOUTNÁ (203 Česká republika, garant, domácí)

Vydání

International Journal of Molecular Sciences, BASEL, Molecular Diversity Preservation International, 2019, 1422-0067

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30402 Technologies involving the manipulation of cells, tissues, organs or the whole organism

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.556

Kód RIV

RIV/00216224:14110/19:00108522

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.3390/ijms20143536

UT WoS

000480449300160

Klíčová slova anglicky

superparamagnetic iron-oxide nanoparticles; human induced pluripotent stem cell-derived endothelial cells; reprogramming; differentiation; mature endothelial cells

Štítky

14110120, 14110517, cbia-web, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 10. 2022 12:50, Mgr. Marie Šípková, DiS.

Anotace

V originále

Background: Endothelial progenitor cells (EPCs) were indicated in vascular repair, angiogenesis of ischemic organs, and inhibition of formation of initial hyperplasia. Differentiation of endothelial cells (ECs) from human induced pluripotent stem cells (hiPSC)-derived endothelial cells (hiPSC-ECs) provides an unlimited supply for clinical application. Furthermore, magnetic cell labelling offers an effective way of targeting and visualization of hiPSC-ECs and is the next step towards in vivo studies. Methods: ECs were differentiated from hiPSCs and labelled with uncoated superparamagnetic iron-oxide nanoparticles (uSPIONs). uSPION uptake was compared between hiPSC-ECs and mature ECs isolated from patients by software analysis of microscopy pictures after Prussian blue cell staining. The acute and long-term cytotoxic effects of uSPIONs were evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay) and Annexin assay. Results: We showed, for the first time, uptake of uncoated SPIONs (uSPIONs) by hiPSC-ECs. In comparison with mature ECs of identical genetic background hiPSC-ECs showed lower uSPION uptake. However, all the studied endothelial cells were effectively labelled and showed magnetic properties even with low labelling concentration of uSPIONs. uSPIONs prepared by microwave plasma synthesis did not show any cytotoxicity nor impair endothelial properties. Conclusion: We show that hiPSC-ECs labelling with low concentration of uSPIONs is feasible and does not show any toxic effects in vitro, which is an important step towards animal studies.

Návaznosti

NV16-31501A, projekt VaV

Název: Tkáňové inženýrství epitelů: Buňky a protokoly pro regenerativní medicínu

Citovat

ŠALINGOVÁ, Barbara, Pavel ŠIMARA, Pavel MATULA, Lenka ZAJÍČKOVÁ, Petr SYNEK, Ondřej JAŠEK, Lenka VEVERKOVÁ, Miroslava SEDLÁČKOVÁ, Zuzana NICHTOVA a Irena KOUTNÁ. The Effect of Uncoated SPIONs on hiPSC-Differentiated Endothelial Cells. International Journal of Molecular Sciences. BASEL: Molecular Diversity Preservation International, 2019, roč. 20, č. 14, s. 1-16. ISSN 1422-0067. Dostupné z: https://dx.doi.org/10.3390/ijms20143536.

@article{1568956,
   author = {Šalingová, Barbara and Šimara, Pavel and Matula, Pavel and Zajíčková, Lenka and Synek, Petr and Jašek, Ondřej and Veverková, Lenka and Sedláčková, Miroslava and Nichtova, Zuzana and Koutná, Irena},
   article_location = {BASEL},
   article_number = {14},
   doi = {http://dx.doi.org/10.3390/ijms20143536},
   keywords = {superparamagnetic iron-oxide nanoparticles; human induced pluripotent stem cell-derived endothelial cells; reprogramming; differentiation; mature endothelial cells},
   language = {eng},
   issn = {1422-0067},
   journal = {International Journal of Molecular Sciences},
   title = {The Effect of Uncoated SPIONs on hiPSC-Differentiated Endothelial Cells},
   url = {https://www.mdpi.com/1422-0067/20/14/3536},
   volume = {20},
   year = {2019}
}

TY  - JOUR
ID  - 1568956
AU  - Šalingová, Barbara - Šimara, Pavel - Matula, Pavel - Zajíčková, Lenka - Synek, Petr - Jašek, Ondřej - Veverková, Lenka - Sedláčková, Miroslava - Nichtova, Zuzana - Koutná, Irena
PY  - 2019
TI  - The Effect of Uncoated SPIONs on hiPSC-Differentiated Endothelial Cells
JF  - International Journal of Molecular Sciences
VL  - 20
IS  - 14
SP  - 1-16
EP  - 1-16
PB  - Molecular Diversity Preservation International
SN  - 14220067
KW  - superparamagnetic iron-oxide nanoparticles
KW  - human induced pluripotent stem cell-derived endothelial cells
KW  - reprogramming
KW  - differentiation
KW  - mature endothelial cells
UR  - https://www.mdpi.com/1422-0067/20/14/3536
L2  - https://www.mdpi.com/1422-0067/20/14/3536
N2  - Background: Endothelial progenitor cells (EPCs) were indicated in vascular repair, angiogenesis of ischemic organs, and inhibition of formation of initial hyperplasia. Differentiation of endothelial cells (ECs) from human induced pluripotent stem cells (hiPSC)-derived endothelial cells (hiPSC-ECs) provides an unlimited supply for clinical application. Furthermore, magnetic cell labelling offers an effective way of targeting and visualization of hiPSC-ECs and is the next step towards in vivo studies. Methods: ECs were differentiated from hiPSCs and labelled with uncoated superparamagnetic iron-oxide nanoparticles (uSPIONs). uSPION uptake was compared between hiPSC-ECs and mature ECs isolated from patients by software analysis of microscopy pictures after Prussian blue cell staining. The acute and long-term cytotoxic effects of uSPIONs were evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay) and Annexin assay. Results: We showed, for the first time, uptake of uncoated SPIONs (uSPIONs) by hiPSC-ECs. In comparison with mature ECs of identical genetic background hiPSC-ECs showed lower uSPION uptake. However, all the studied endothelial cells were effectively labelled and showed magnetic properties even with low labelling concentration of uSPIONs. uSPIONs prepared by microwave plasma synthesis did not show any cytotoxicity nor impair endothelial properties. Conclusion: We show that hiPSC-ECs labelling with low concentration of uSPIONs is feasible and does not show any toxic effects in vitro, which is an important step towards animal studies.
ER  -

ŠALINGOVÁ, Barbara, Pavel ŠIMARA, Pavel MATULA, Lenka ZAJÍČKOVÁ, Petr SYNEK, Ondřej JAŠEK, Lenka VEVERKOVÁ, Miroslava SEDLÁČKOVÁ, Zuzana NICHTOVA a Irena KOUTNÁ. The Effect of Uncoated SPIONs on hiPSC-Differentiated Endothelial Cells. \textit{International Journal of Molecular Sciences}. BASEL: Molecular Diversity Preservation International, 2019, roč.~20, č.~14, s.~1-16. ISSN~1422-0067. Dostupné z: https://dx.doi.org/10.3390/ijms20143536.

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