Sodium/calcium exchanger is involved in apoptosis induced by
H2S in tumor cells through decreased levels of intracellular pH

J 2019

Sodium/calcium exchanger is involved in apoptosis induced by H2S in tumor cells through decreased levels of intracellular pH

SZADVÁRI, Ivan, Soňa HUDECOVÁ, Barbora CHOVANCOVÁ, Miroslava MATÚŠKOVÁ, Dana CHOLUJOVÁ et. al.

Základní údaje

Originální název

Sodium/calcium exchanger is involved in apoptosis induced by H2S in tumor cells through decreased levels of intracellular pH

Autoři

SZADVÁRI, Ivan (703 Slovensko, domácí), Soňa HUDECOVÁ (703 Slovensko), Barbora CHOVANCOVÁ (703 Slovensko), Miroslava MATÚŠKOVÁ (703 Slovensko), Dana CHOLUJOVÁ (703 Slovensko), Ľubomíra LENČEŠOVA (703 Slovensko), David VALERIÁN (203 Česká republika, domácí), Karol ONDRIAS (703 Slovensko), Petr BABULA (203 Česká republika, domácí) a Oľga KRIŽANOVÁ (703 Slovensko, garant, domácí)

Vydání

NITRIC OXIDE-BIOLOGY AND CHEMISTRY, SAN DIEGO, ACADEMIC PRESS INC ELSEVIER SCIENCE, 2019, 1089-8603

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.311

Kód RIV

RIV/00216224:14110/19:00111015

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1016/j.niox.2019.02.011

UT WoS

000466832100001

Klíčová slova anglicky

Apoptosis; Hydrogen sulfide; Intracellular acidification; Sodium/calcium exchanger; Sodium/hydrogen exchanger

Štítky

14110515, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 14. 4. 2020 14:37, Mgr. Tereza Miškechová

Anotace

V originále

We explored possibility that sodium/calcium exchanger 1 (NCX1) is involved in pH modulation and apoptosis induction in GYY4137 treated cells. We have shown that although 10 days treatment with GYY4137 did not significantly decreased volume of tumors induced by colorectal cancer DLD1 cells in nude mice, it already induced apoptosis in these tumors. Treatment of DLD1 and ovarian cancer A2780 cells with GYY4137 resulted in intracellular acidification in a concentration-dependent manner. We observed increased mRNA and protein expression of both, NCX1 and sodium/hydrogen exchanger 1 (NHE1) in DLD1-induced tumors from GYY4137-treated mice. NCX1 was coupled with NHE1 in A2780 and DLD1 cells and this complex partially disintegrated after GYY4137 treatment. We proposed that intracellular acidification is due to uncoupling of NCX1/NHE1 complex rather than blocking of the reverse mode of NCX1, probably due to internalization of NHE1. Results might contribute to understanding molecular mechanism of H2S induced apoptosis in tumor cells.

Návaznosti

MUNI/A/1255/2018, interní kód MU

Název: Kardiovaskulární systém a jeho regulace a dysregulace pod vlivem farmak (Akronym: KAREDYSFAR)

Investor: Masarykova univerzita, Kardiovaskulární systém a jeho regulace a dysregulace pod vlivem farmak, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

SZADVÁRI, Ivan, Soňa HUDECOVÁ, Barbora CHOVANCOVÁ, Miroslava MATÚŠKOVÁ, Dana CHOLUJOVÁ, Ľubomíra LENČEŠOVA, David VALERIÁN, Karol ONDRIAS, Petr BABULA a Oľga KRIŽANOVÁ. Sodium/calcium exchanger is involved in apoptosis induced by H2S in tumor cells through decreased levels of intracellular pH. NITRIC OXIDE-BIOLOGY AND CHEMISTRY. SAN DIEGO: ACADEMIC PRESS INC ELSEVIER SCIENCE, 2019, roč. 87, JUN 1 2019, s. 1-9. ISSN 1089-8603. Dostupné z: https://dx.doi.org/10.1016/j.niox.2019.02.011.

@article{1572158,
   author = {Szadvári, Ivan and Hudecová, Soňa and Chovancová, Barbora and Matúšková, Miroslava and Cholujová, Dana and Lenčešova, Ľubomíra and Valerián, David and Ondrias, Karol and Babula, Petr and Križanová, Oľga},
   article_location = {SAN DIEGO},
   article_number = {JUN 1 2019},
   doi = {http://dx.doi.org/10.1016/j.niox.2019.02.011},
   keywords = {Apoptosis; Hydrogen sulfide; Intracellular acidification; Sodium/calcium exchanger; Sodium/hydrogen exchanger},
   language = {eng},
   issn = {1089-8603},
   journal = {NITRIC OXIDE-BIOLOGY AND CHEMISTRY},
   title = {Sodium/calcium exchanger is involved in apoptosis induced by H2S in tumor cells through decreased levels of intracellular pH},
   url = {https://www.sciencedirect.com/science/article/pii/S1089860318303343?via%3Dihub},
   volume = {87},
   year = {2019}
}

TY  - JOUR
ID  - 1572158
AU  - Szadvári, Ivan - Hudecová, Soňa - Chovancová, Barbora - Matúšková, Miroslava - Cholujová, Dana - Lenčešova, Ľubomíra - Valerián, David - Ondrias, Karol - Babula, Petr - Križanová, Oľga
PY  - 2019
TI  - Sodium/calcium exchanger is involved in apoptosis induced by H2S in tumor cells through decreased levels of intracellular pH
JF  - NITRIC OXIDE-BIOLOGY AND CHEMISTRY
VL  - 87
IS  - JUN 1 2019
SP  - 1-9
EP  - 1-9
PB  - ACADEMIC PRESS INC ELSEVIER SCIENCE
SN  - 10898603
KW  - Apoptosis
KW  - Hydrogen sulfide
KW  - Intracellular acidification
KW  - Sodium/calcium exchanger
KW  - Sodium/hydrogen exchanger
UR  - https://www.sciencedirect.com/science/article/pii/S1089860318303343?via%3Dihub
L2  - https://www.sciencedirect.com/science/article/pii/S1089860318303343?via%3Dihub
N2  - We explored possibility that sodium/calcium exchanger 1 (NCX1) is involved in pH modulation and apoptosis induction in GYY4137 treated cells. We have shown that although 10 days treatment with GYY4137 did not significantly decreased volume of tumors induced by colorectal cancer DLD1 cells in nude mice, it already induced apoptosis in these tumors. Treatment of DLD1 and ovarian cancer A2780 cells with GYY4137 resulted in intracellular acidification in a concentration-dependent manner. We observed increased mRNA and protein expression of both, NCX1 and sodium/hydrogen exchanger 1 (NHE1) in DLD1-induced tumors from GYY4137-treated mice. NCX1 was coupled with NHE1 in A2780 and DLD1 cells and this complex partially disintegrated after GYY4137 treatment. We proposed that intracellular acidification is due to uncoupling of NCX1/NHE1 complex rather than blocking of the reverse mode of NCX1, probably due to internalization of NHE1. Results might contribute to understanding molecular mechanism of H2S induced apoptosis in tumor cells.
ER  -

SZADVÁRI, Ivan, Soňa HUDECOVÁ, Barbora CHOVANCOVÁ, Miroslava MATÚŠKOVÁ, Dana CHOLUJOVÁ, Ľubomíra LENČEŠOVA, David VALERIÁN, Karol ONDRIAS, Petr BABULA a Oľga KRIŽANOVÁ. Sodium/calcium exchanger is involved in apoptosis induced by H2S in tumor cells through decreased levels of intracellular pH. \textit{NITRIC OXIDE-BIOLOGY AND CHEMISTRY}. SAN DIEGO: ACADEMIC PRESS INC ELSEVIER SCIENCE, 2019, roč.~87, JUN 1 2019, s.~1-9. ISSN~1089-8603. Dostupné z: https://dx.doi.org/10.1016/j.niox.2019.02.011.

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