| HREBÍK, Dominik, Dana ŠTVERÁKOVÁ, Karel ŠKUBNÍK, Tibor FÜZIK, Roman PANTŮČEK a Pavel PLEVKA. Structure and genome ejection mechanism of Staphylococcus aureus phage P68. Science advances. New York: American Association for the Advancement of Science, 2019, roč. 5, č. 10, s. "eaaw7414", 14 s. ISSN 2375-2548. Dostupné z: https://dx.doi.org/10.1126/sciadv.aaw7414. |
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@article{1574236,
author = {Hrebík, Dominik and Štveráková, Dana and Škubník, Karel and Füzik, Tibor and Pantůček, Roman and Plevka, Pavel},
article_location = {New York},
article_number = {10},
doi = {http://dx.doi.org/10.1126/sciadv.aaw7414},
keywords = {Podoviridae; RECEPTOR-BINDING PROTEIN; CRYSTAL-STRUCTURE; CRYO-EM; BACTERIOPHAGE P68},
language = {eng},
issn = {2375-2548},
journal = {Science advances},
title = {Structure and genome ejection mechanism of Staphylococcus aureus phage P68},
url = {https://advances.sciencemag.org/content/5/10/eaaw7414},
volume = {5},
year = {2019}
}
TY - JOUR
ID - 1574236
AU - Hrebík, Dominik - Štveráková, Dana - Škubník, Karel - Füzik, Tibor - Pantůček, Roman - Plevka, Pavel
PY - 2019
TI - Structure and genome ejection mechanism of Staphylococcus aureus phage P68
JF - Science advances
VL - 5
IS - 10
SP - "eaaw7414"
EP - "eaaw7414"
PB - American Association for the Advancement of Science
SN - 23752548
KW - Podoviridae
KW - RECEPTOR-BINDING PROTEIN
KW - CRYSTAL-STRUCTURE
KW - CRYO-EM
KW - BACTERIOPHAGE P68
UR - https://advances.sciencemag.org/content/5/10/eaaw7414
L2 - https://advances.sciencemag.org/content/5/10/eaaw7414
N2 - Phages infecting Staphylococcus aureus can be used as therapeutics against antibiotic-resistant bacterial infections. However, there is limited information about the mechanism of genome delivery of phages that infect Gram-positive bacteria. Here, we present the structures of native S. aureus phage P68, genome ejection intermediate, and empty particle. The P68 head contains 72 subunits of inner core protein, 15 of which bind to and alter the structure of adjacent major capsid proteins and thus specify attachment sites for head fibers. Unlike in the previously studied phages, the head fibers of P68 enable its virion to position itself at the cell surface for genome delivery. The unique interaction of one end of P68 DNA with one of the 12 portal protein subunits is disrupted before the genome ejection. The inner core proteins are released together with the DNA and enable the translocation of phage genome across the bacterial membrane into the cytoplasm.
ER -
HREBÍK, Dominik, Dana ŠTVERÁKOVÁ, Karel ŠKUBNÍK, Tibor FÜZIK, Roman PANTŮČEK a Pavel PLEVKA. Structure and genome ejection mechanism of Staphylococcus aureus phage P68. \textit{Science advances}. New York: American Association for the Advancement of Science, 2019, roč.~5, č.~10, s.~''eaaw7414'', 14 s. ISSN~2375-2548. Dostupné z: https://dx.doi.org/10.1126/sciadv.aaw7414.
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