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@article{1576081, author = {Trnková, Libuše and Třísková, Iveta and Vorlíčková, Michaela and Kejnovská, Iva and Dvořáková, Zuzana and Pivoňková, Hana and Fiala, Radovan}, article_number = {10}, doi = {http://dx.doi.org/10.1002/elan.201900323}, keywords = {i-motif; (C)-rich DNA nonamers; linear sweep voltammetry; elimination voltammetry with linear scan; CD spectra; NMR spectra; native PAGE}, language = {eng}, issn = {1040-0397}, journal = {Electroanalysis}, title = {Comparative Electrochemical and Spectroscopic Studies of I-motif forming DNA Nonamers}, url = {https://onlinelibrary.wiley.com/doi/epdf/10.1002/elan.201900323}, volume = {31}, year = {2019} }
TY - JOUR ID - 1576081 AU - Trnková, Libuše - Třísková, Iveta - Vorlíčková, Michaela - Kejnovská, Iva - Dvořáková, Zuzana - Pivoňková, Hana - Fiala, Radovan PY - 2019 TI - Comparative Electrochemical and Spectroscopic Studies of I-motif forming DNA Nonamers JF - Electroanalysis VL - 31 IS - 10 SP - 2081-2093 EP - 2081-2093 SN - 10400397 KW - i-motif KW - (C)-rich DNA nonamers KW - linear sweep voltammetry KW - elimination voltammetry with linear scan KW - CD spectra KW - NMR spectra KW - native PAGE UR - https://onlinelibrary.wiley.com/doi/epdf/10.1002/elan.201900323 L2 - https://onlinelibrary.wiley.com/doi/epdf/10.1002/elan.201900323 N2 - The paper shows the structural diversity of cytosine (C)-rich oligodeoxynucleotides (ODNs) arising from their detail nucleotide sequence and experimental conditions. In slightly acidic solutions, the ODN nonamers with different adenine (A) and cytosine (C) sequences can adopt non-canonical structures involving protonated bases. A distinct secondary structure formed in (C)-rich sequences, called i-motif (iM), consists of hemiprotonated and intercalated cytosine base pairs (C.C+). Folding and unfolding of particular structures in solutions were monitored by H-1 NMR and CD spectroscopies and native polyacrylamide gel electrophoresis (PAGE), which are capable to determine their structural characteristics. Effects of sequences and their proclivity to formation of the iM on electrochemical behaviour of the ODN nonamers were studied by electrochemical methods. The LSV signals of A and C obtained from the reductive dissolution of ODN adsorption layers on a hanging mercury drop electrode were processed by elimination voltammetry with linear scan (EVLS), which revealed complex effects of the nonamer properties (namely their primary and secondary structure confirmed in solution) on their adsorption and reduction activity. ER -
TRNKOVÁ, Libuše, Iveta TŘÍSKOVÁ, Michaela VORLÍČKOVÁ, Iva KEJNOVSKÁ, Zuzana DVOŘÁKOVÁ, Hana PIVOŇKOVÁ a Radovan FIALA. Comparative Electrochemical and Spectroscopic Studies of I-motif forming DNA Nonamers. \textit{Electroanalysis}. 2019, roč.~31, č.~10, s.~2081-2093. ISSN~1040-0397. Dostupné z: https://dx.doi.org/10.1002/elan.201900323.
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