Comparative Electrochemical and Spectroscopic Studies of
I-motif forming DNA Nonamers

J 2019

Comparative Electrochemical and Spectroscopic Studies of I-motif forming DNA Nonamers

TRNKOVÁ, Libuše, Iveta TŘÍSKOVÁ, Michaela VORLÍČKOVÁ, Iva KEJNOVSKÁ, Zuzana DVOŘÁKOVÁ et. al.

Základní údaje

Originální název

Comparative Electrochemical and Spectroscopic Studies of I-motif forming DNA Nonamers

Autoři

TRNKOVÁ, Libuše (203 Česká republika, garant, domácí), Iveta TŘÍSKOVÁ (203 Česká republika, domácí), Michaela VORLÍČKOVÁ (203 Česká republika), Iva KEJNOVSKÁ (203 Česká republika), Zuzana DVOŘÁKOVÁ (203 Česká republika), Hana PIVOŇKOVÁ (203 Česká republika) a Radovan FIALA (203 Česká republika, domácí)

Vydání

Electroanalysis, 2019, 1040-0397

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10400 1.4 Chemical sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

https://onlinelibrary.wiley.com/doi/epdf/10.1002/elan.201900323

Impakt faktor

Impact factor: 2.544

Kód RIV

RIV/00216224:14310/19:00111161

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1002/elan.201900323

UT WoS

000488393000001

Klíčová slova anglicky

i-motif; (C)-rich DNA nonamers; linear sweep voltammetry; elimination voltammetry with linear scan; CD spectra; NMR spectra; native PAGE

Štítky

CF NMR

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 30. 10. 2024 14:03, Ing. Martina Blahová

Anotace

V originále

The paper shows the structural diversity of cytosine (C)-rich oligodeoxynucleotides (ODNs) arising from their detail nucleotide sequence and experimental conditions. In slightly acidic solutions, the ODN nonamers with different adenine (A) and cytosine (C) sequences can adopt non-canonical structures involving protonated bases. A distinct secondary structure formed in (C)-rich sequences, called i-motif (iM), consists of hemiprotonated and intercalated cytosine base pairs (C.C+). Folding and unfolding of particular structures in solutions were monitored by H-1 NMR and CD spectroscopies and native polyacrylamide gel electrophoresis (PAGE), which are capable to determine their structural characteristics. Effects of sequences and their proclivity to formation of the iM on electrochemical behaviour of the ODN nonamers were studied by electrochemical methods. The LSV signals of A and C obtained from the reductive dissolution of ODN adsorption layers on a hanging mercury drop electrode were processed by elimination voltammetry with linear scan (EVLS), which revealed complex effects of the nonamer properties (namely their primary and secondary structure confirmed in solution) on their adsorption and reduction activity.

Návaznosti

MUNI/A/1359/2018, interní kód MU

Název: Vývoj metod a instrumentace pro analýzu biologicky významných látek 2019

Investor: Masarykova univerzita, Vývoj metod a instrumentace pro analýzu biologicky významných látek 2019, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

90043, velká výzkumná infrastruktura

Název: CIISB

Citovat

TRNKOVÁ, Libuše, Iveta TŘÍSKOVÁ, Michaela VORLÍČKOVÁ, Iva KEJNOVSKÁ, Zuzana DVOŘÁKOVÁ, Hana PIVOŇKOVÁ a Radovan FIALA. Comparative Electrochemical and Spectroscopic Studies of I-motif forming DNA Nonamers. Electroanalysis. 2019, roč. 31, č. 10, s. 2081-2093. ISSN 1040-0397. Dostupné z: https://dx.doi.org/10.1002/elan.201900323.

@article{1576081,
   author = {Trnková, Libuše and Třísková, Iveta and Vorlíčková, Michaela and Kejnovská, Iva and Dvořáková, Zuzana and Pivoňková, Hana and Fiala, Radovan},
   article_number = {10},
   doi = {http://dx.doi.org/10.1002/elan.201900323},
   keywords = {i-motif; (C)-rich DNA nonamers; linear sweep voltammetry; elimination voltammetry with linear scan; CD spectra; NMR spectra; native PAGE},
   language = {eng},
   issn = {1040-0397},
   journal = {Electroanalysis},
   title = {Comparative Electrochemical and Spectroscopic Studies of I-motif forming DNA Nonamers},
   url = {https://onlinelibrary.wiley.com/doi/epdf/10.1002/elan.201900323},
   volume = {31},
   year = {2019}
}

TY  - JOUR
ID  - 1576081
AU  - Trnková, Libuše - Třísková, Iveta - Vorlíčková, Michaela - Kejnovská, Iva - Dvořáková, Zuzana - Pivoňková, Hana - Fiala, Radovan
PY  - 2019
TI  - Comparative Electrochemical and Spectroscopic Studies of I-motif forming DNA Nonamers
JF  - Electroanalysis
VL  - 31
IS  - 10
SP  - 2081-2093
EP  - 2081-2093
SN  - 10400397
KW  - i-motif
KW  - (C)-rich DNA nonamers
KW  - linear sweep voltammetry
KW  - elimination voltammetry with linear scan
KW  - CD spectra
KW  - NMR spectra
KW  - native PAGE
UR  - https://onlinelibrary.wiley.com/doi/epdf/10.1002/elan.201900323
L2  - https://onlinelibrary.wiley.com/doi/epdf/10.1002/elan.201900323
N2  - The paper shows the structural diversity of cytosine (C)-rich oligodeoxynucleotides (ODNs) arising from their detail nucleotide sequence and experimental conditions. In slightly acidic solutions, the ODN nonamers with different adenine (A) and cytosine (C) sequences can adopt non-canonical structures involving protonated bases. A distinct secondary structure formed in (C)-rich sequences, called i-motif (iM), consists of hemiprotonated and intercalated cytosine base pairs (C.C+). Folding and unfolding of particular structures in solutions were monitored by H-1 NMR and CD spectroscopies and native polyacrylamide gel electrophoresis (PAGE), which are capable to determine their structural characteristics. Effects of sequences and their proclivity to formation of the iM on electrochemical behaviour of the ODN nonamers were studied by electrochemical methods. The LSV signals of A and C obtained from the reductive dissolution of ODN adsorption layers on a hanging mercury drop electrode were processed by elimination voltammetry with linear scan (EVLS), which revealed complex effects of the nonamer properties (namely their primary and secondary structure confirmed in solution) on their adsorption and reduction activity.
ER  -

TRNKOVÁ, Libuše, Iveta TŘÍSKOVÁ, Michaela VORLÍČKOVÁ, Iva KEJNOVSKÁ, Zuzana DVOŘÁKOVÁ, Hana PIVOŇKOVÁ a Radovan FIALA. Comparative Electrochemical and Spectroscopic Studies of I-motif forming DNA Nonamers. \textit{Electroanalysis}. 2019, roč.~31, č.~10, s.~2081-2093. ISSN~1040-0397. Dostupné z: https://dx.doi.org/10.1002/elan.201900323.

Podrobný výpis o publikaci