5. Influence of selected cryoprotectants on regeneration of cryopreserved cells properties

JELÍNKOVÁ, Šárka, I KRATOCHVÍLOVÁ, Martin PEŠL, Jan PŘIBYL, Petr SKLÁDAL, Vladimír ROTREKL a Jan PŘIBYL. 5. Influence of selected cryoprotectants on regeneration of cryopreserved cells properties. Online. In Haider Khawaja. Stem Cells: From Myth to Reality and Evolving. 1. vyd. Springer, 2019, s. 102-126, 24 s. ISBN 978-3-11-064243-8. Dostupné z: https://dx.doi.org/10.1515/9783110642438-005.

Základní údaje

Originální název

5. Influence of selected cryoprotectants on regeneration of cryopreserved cells properties

Název anglicky

5. Influence of selected cryoprotectants on regeneration of cryopreserved cells properties

Autoři

JELÍNKOVÁ, Šárka, I KRATOCHVÍLOVÁ, Martin PEŠL, Jan PŘIBYL, Petr SKLÁDAL, Vladimír ROTREKL a Jan PŘIBYL

Vydání

1. vyd. Stem Cells: From Myth to Reality and Evolving, od s. 102-126, 24 s. 2019

Nakladatel

Springer

---

Další údaje

Typ výsledku

Kapitola resp. kapitoly v odborné knize

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

elektronická verze "online"

ISBN

978-3-11-064243-8

DOI

http://dx.doi.org/10.1515/9783110642438-005

Klíčová slova anglicky

Cryopreservation, Cryoprotectants, Freeze, Multipotent, Pluripotent, Saccharose, Stem cells, Therapy, Trehalose.

Štítky

topvydavatel

---

Anotace

V originále

The cryopreservation of cells is a fundamental task for a wide range of applications, from basic storage of cells to fertilization. Cells must be protected against damage during freezing by application of specifíc cryoprotectants and freezing/melting protocols. This chapter presents a brief overview of the predominant cryopreservation methods and most common cryoprotectants. Furthermore, it explores the specifics of cryopreservation of stem cells with various types and levels of multipotency. Special attention is dedicated to the effect of cryopreservation on human pluripotent stem cells, including the effect of different cryoprotectants used to prepare fibroblast feeder layer for the cultivation of pluripotent cells. Finally, the chapter concludes with a review of selected methods that have been successfully used to probe the postthaw state of cryopreserved cells through their viability, inner structure, and mechanical properties.

Anglicky

The cryopreservation of cells is a fundamental task for a wide range of applications, from basic storage of cells to fertilization. Cells must be protected against damage during freezing by application of specifíc cryoprotectants and freezing/melting protocols. This chapter presents a brief overview of the predominant cryopreservation methods and most common cryoprotectants. Furthermore, it explores the specifics of cryopreservation of stem cells with various types and levels of multipotency. Special attention is dedicated to the effect of cryopreservation on human pluripotent stem cells, including the effect of different cryoprotectants used to prepare fibroblast feeder layer for the cultivation of pluripotent cells. Finally, the chapter concludes with a review of selected methods that have been successfully used to probe the postthaw state of cryopreserved cells through their viability, inner structure, and mechanical properties.