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@inbook{1580237,
author = {Jelínková, Šárka and Kratochvílová, I and Pešl, Martin and Přibyl, Jan and Skládal, Petr and Rotrekl, Vladimír and Přibyl, Jan},
booktitle = {Stem Cells: From Myth to Reality and Evolving},
edition = {1},
editor = {Haider Khawaja},
keywords = {Cryopreservation, rCyoprotectants, Freeze, Multipotent, Pluripotent, Saccharose, Stem cells, Therapy, Trehalose.},
howpublished = {elektronická verze "online"},
pages = {102-126},
publisher = {Springer},
title = {5. Influence of selected cryoprotectants on regeneration of cryopreserved cells properties},
year = {2019}
}
TY - CHAP
ID - 1580237
AU - Jelínková, Šárka - Kratochvílová, I - Pešl, Martin - Přibyl, Jan - Skládal, Petr - Rotrekl, Vladimír - Přibyl, Jan
PY - 2019
TI - 5. Influence of selected cryoprotectants on regeneration of cryopreserved cells properties
PB - Springer
KW - Cryopreservation, rCyoprotectants, Freeze, Multipotent, Pluripotent, Saccharose, Stem cells, Therapy, Trehalose.
N2 - The cryopreservation of cells is a fundamental task for a wide range of applications, from basic storage of cells to fertilization. Cells must be protected against damage during freezing by application of specifíc cryoprotectants and freezing/melting protocols. This chapter presents a brief overview of the predominant cryopreservation methods and most common cryoprotectants. Furthermore, it explores the specifics of cryopreservation of stem cells with various types and levels of multipotency. Special attention is dedicated to the effect of cryopreservation on human pluripotent stem cells, including the effect of different cryoprotectants used to prepare fibroblast feeder layer for the cultivation of pluripotent cells. Finally, the chapter concludes with a review of selected methods that have been successfully used to probe the postthaw state of cryopreserved cells through their viability, inner structure, and mechanical properties.
ER -
JELÍNKOVÁ, Šárka, I KRATOCHVÍLOVÁ, Martin PEŠL, Jan PŘIBYL, Petr SKLÁDAL, Vladimír ROTREKL a Jan PŘIBYL. 5. Influence of selected cryoprotectants on regeneration of cryopreserved cells properties. Online. In Haider Khawaja. \textit{Stem Cells: From Myth to Reality and Evolving}. 1. vyd. Springer, 2019, s.~102-126, 24 s.
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