Hydrogen Sulfide Effects on the Survival of Lactobacilli with
Emphasis on the Development of Inflammatory Bowel Diseases

J 2019

Hydrogen Sulfide Effects on the Survival of Lactobacilli with Emphasis on the Development of Inflammatory Bowel Diseases

KUSHKEVYCH, Ivan, Věra KOTRSOVÁ, Dani DORDEVIĆ, Leona BUŇKOVÁ, Monika VÍTĚZOVÁ et. al.

Základní údaje

Originální název

Hydrogen Sulfide Effects on the Survival of Lactobacilli with Emphasis on the Development of Inflammatory Bowel Diseases

Autoři

KUSHKEVYCH, Ivan (804 Ukrajina, garant, domácí), Věra KOTRSOVÁ (203 Česká republika, domácí), Dani DORDEVIĆ (203 Česká republika), Leona BUŇKOVÁ (203 Česká republika), Monika VÍTĚZOVÁ (203 Česká republika, domácí) a Amedeo AMEDEI (380 Itálie)

Vydání

Biomolecules, MDPI, 2019, 2218-273X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.082

Kód RIV

RIV/00216224:14310/19:00111414

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.3390/biom9120752

UT WoS

000506636800001

Klíčová slova anglicky

hydrogen sulfide; toxicity; intestinal microbiome; sulfate-reducing bacteria; lactic acid bacteria; inflammatory bowel disease; ulcerative colitis

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 26. 3. 2020 14:20, Mgr. Marie Šípková, DiS.

Anotace

V originále

The gut microbiota is a complex component of humans that depends on diet, host genome, and lifestyle. The background: The study purpose is to find relations between nutrition, intestinal lactic acid bacteria (LAB) from various environments (human, animal intestine, and yogurt) and sulfate-reducing microbial communities in the large intestine; to compare kinetic growth parameters of LAB; and to determine their sensitivity to different concentration of hydrogen sulfide produced by intestinal sulfate-reducing bacteria. Methods: Microbiological (isolation and identification), biochemical (electrophoresis), molecular biology methods (DNA isolation and PCR analysis), and statistical processing (average and standard error calculations) of the results were used. The results: The toxicity of hydrogen sulfide produced by sulfate-reducing bacteria, the survival of lactic acid bacteria, and minimal inhibitory concentrations (MIC) were determined. The measured hydrogen sulfide sensitivity values were the same for L. paracasei and L. reuteri (MIC > 1.1 mM). In addition, L. plantarum and L. fermentum showed also a similar sensitivity (MIC > 0.45 mM) but significantly (p < 0.05) lower than L. reuteri and L. paracasei (1.1 > 0.45 mM). L. paracasei and L. reuteri are more sensitive to hydrogen sulfide than L. fermentum and L. plantarum. L. pentosus was sensitive to the extremely low concentration of H2S (MIC > 0.15 mM). Conclusions: The Lactobacillus species were significantly sensitive to hydrogen sulfide, which is a final metabolite of intestinal sulfate-reducing bacteria. The results are definitely helpful for a better understanding of complicated interaction among intestinal microbiota and nutrition.

Návaznosti

MUNI/A/0902/2018, interní kód MU

Název: Podpora výzkumné činnosti studentů Mikrobiologie a molekulární biotechnologie 3

Investor: Masarykova univerzita, Podpora výzkumné činnosti studentů Mikrobiologie a molekulární biotechnologie 3, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

KUSHKEVYCH, Ivan, Věra KOTRSOVÁ, Dani DORDEVIĆ, Leona BUŇKOVÁ, Monika VÍTĚZOVÁ a Amedeo AMEDEI. Hydrogen Sulfide Effects on the Survival of Lactobacilli with Emphasis on the Development of Inflammatory Bowel Diseases. Biomolecules. MDPI, 2019, roč. 9, č. 12, s. 1-13. ISSN 2218-273X. Dostupné z: https://dx.doi.org/10.3390/biom9120752.

@article{1582160,
   author = {Kushkevych, Ivan and Kotrsová, Věra and Dordević, Dani and Buňková, Leona and Vítězová, Monika and Amedei, Amedeo},
   article_number = {12},
   doi = {http://dx.doi.org/10.3390/biom9120752},
   keywords = {hydrogen sulfide; toxicity; intestinal microbiome; sulfate-reducing bacteria; lactic acid bacteria; inflammatory bowel disease; ulcerative colitis},
   language = {eng},
   issn = {2218-273X},
   journal = {Biomolecules},
   title = {Hydrogen Sulfide Effects on the Survival of Lactobacilli with Emphasis on the Development of Inflammatory Bowel Diseases},
   url = {https://www.mdpi.com/2218-273X/9/12/752},
   volume = {9},
   year = {2019}
}

TY  - JOUR
ID  - 1582160
AU  - Kushkevych, Ivan - Kotrsová, Věra - Dordević, Dani - Buňková, Leona - Vítězová, Monika - Amedei, Amedeo
PY  - 2019
TI  - Hydrogen Sulfide Effects on the Survival of Lactobacilli with Emphasis on the Development of Inflammatory Bowel Diseases
JF  - Biomolecules
VL  - 9
IS  - 12
SP  - 1-13
EP  - 1-13
PB  - MDPI
SN  - 2218273X
KW  - hydrogen sulfide
KW  - toxicity
KW  - intestinal microbiome
KW  - sulfate-reducing bacteria
KW  - lactic acid bacteria
KW  - inflammatory bowel disease
KW  - ulcerative colitis
UR  - https://www.mdpi.com/2218-273X/9/12/752
L2  - https://www.mdpi.com/2218-273X/9/12/752
N2  - The gut microbiota is a complex component of humans that depends on diet, host genome, and lifestyle. The background: The study purpose is to find relations between nutrition, intestinal lactic acid bacteria (LAB) from various environments (human, animal intestine, and yogurt) and sulfate-reducing microbial communities in the large intestine; to compare kinetic growth parameters of LAB; and to determine their sensitivity to different concentration of hydrogen sulfide produced by intestinal sulfate-reducing bacteria. Methods: Microbiological (isolation and identification), biochemical (electrophoresis), molecular biology methods (DNA isolation and PCR analysis), and statistical processing (average and standard error calculations) of the results were used. The results: The toxicity of hydrogen sulfide produced by sulfate-reducing bacteria, the survival of lactic acid bacteria, and minimal inhibitory concentrations (MIC) were determined. The measured hydrogen sulfide sensitivity values were the same for L. paracasei and L. reuteri (MIC > 1.1 mM). In addition, L. plantarum and L. fermentum showed also a similar sensitivity (MIC > 0.45 mM) but significantly (p < 0.05) lower than L. reuteri and L. paracasei (1.1 > 0.45 mM). L. paracasei and L. reuteri are more sensitive to hydrogen sulfide than L. fermentum and L. plantarum. L. pentosus was sensitive to the extremely low concentration of H2S (MIC > 0.15 mM). Conclusions: The Lactobacillus species were significantly sensitive to hydrogen sulfide, which is a final metabolite of intestinal sulfate-reducing bacteria. The results are definitely helpful for a better understanding of complicated interaction among intestinal microbiota and nutrition.
ER  -

KUSHKEVYCH, Ivan, Věra KOTRSOVÁ, Dani DORDEVI$\backslash$'C, Leona BUŇKOVÁ, Monika VÍTĚZOVÁ a Amedeo AMEDEI. Hydrogen Sulfide Effects on the Survival of Lactobacilli with Emphasis on the Development of Inflammatory Bowel Diseases. \textit{Biomolecules}. MDPI, 2019, roč.~9, č.~12, s.~1-13. ISSN~2218-273X. Dostupné z: https://dx.doi.org/10.3390/biom9120752.

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