One-step detection of human papilloma viral infection using
quantum dot-nucleotide interaction specificity

J 2019

One-step detection of human papilloma viral infection using quantum dot-nucleotide interaction specificity

JIMENEZ, Ana Maria Jimenez, Amitava MOULICK, Sukanya BHOWMICK, Vladislav STRMISKA, Milica GAGIC et. al.

Základní údaje

Originální název

One-step detection of human papilloma viral infection using quantum dot-nucleotide interaction specificity

Autoři

JIMENEZ, Ana Maria Jimenez (203 Česká republika), Amitava MOULICK (203 Česká republika), Sukanya BHOWMICK (203 Česká republika), Vladislav STRMISKA (203 Česká republika), Milica GAGIC (203 Česká republika), Zuzana HORAKOVA (203 Česká republika), Rom KOSTRICA (203 Česká republika), Michal MASAŘÍK (203 Česká republika, domácí), Zbynek HEGER (203 Česká republika) a Vojtech ADAM (203 Česká republika, garant)

Vydání

Talanta, AMSTERDAM, Elsevier, 2019, 0039-9140

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30204 Oncology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.339

Kód RIV

RIV/00216224:14110/19:00107803

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1016/j.talanta.2019.07.006

UT WoS

000485856500047

Klíčová slova anglicky

Cancer; Human papillomavirus; Nanotechnology; Magnetic isolation; Quantum dots

Štítky

14110518, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 20. 11. 2019 14:16, Mgr. Tereza Miškechová

Anotace

V originále

Due to the close relationship between carcinogenesis and human papillomavirus (HPV), and since they are transmitted via huge number of asymptomatic carriers, the detection of HPV is really needed to reduce the risk of developing cancer. According to the best of our knowledge, our study provides the very first method for one-step detection of viral infection and if it has initiated the subsequent cancer proliferation. The proposed novel nanosystem consists of magnetic glass particles (MGPs), which were attached with DNA probe on their surface to hybridize with target DNAs. The MGP-probe-DNA hybrid was finally conjugated with CdTe/ZnSe core/shell quantum dots (QDs). The proposed detection system is based on a novel mechanism in which the MGPs separate out the target DNAs from different biological samples using external magnetic field for better and clear detection and the QDs give different fluorescent maxima for different target DNAs due to their ability to interact differently with different nucleotides. Firstly, the method was optimized using HPV genes cloned into synthetic plasmids. Then it was applied directly on the samples from normal and cancerous cells. After that, the real hospital samples of head and neck squamous cell carcinoma (HNSCC) with or without the infection of HPV were also analyzed. Our novel nano-system is proved successful in detecting and distinguishing between the patients suffering by HPV infection with or without subsequent cancer having detection limit estimated as 1.0 x 10(9) (GEq/mL). The proposed methodology is faster and cost-effective, which can be applied at the clinical level to help the doctors to decide the strategy of medication that may save the life of the patients with an early treatment.

Návaznosti

GA18-03978S, projekt VaV

Název: Efekt buněčné tuhosti a mikroprostředí jako prediktor odpovědi na léčbu karcinomů hlavy a krku

Investor: Grantová agentura ČR, Efekt buněčné tuhosti a mikroprostředí jako prediktor odpovědi na léčbu karcinomů hlavy a krku

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

NV16-29835A, projekt VaV

Název: Molekulárně-genetické markery predikce účinnosti radioterapie u nádorů hlavy a krku

Citovat

JIMENEZ, Ana Maria Jimenez, Amitava MOULICK, Sukanya BHOWMICK, Vladislav STRMISKA, Milica GAGIC, Zuzana HORAKOVA, Rom KOSTRICA, Michal MASAŘÍK, Zbynek HEGER a Vojtech ADAM. One-step detection of human papilloma viral infection using quantum dot-nucleotide interaction specificity. Talanta. AMSTERDAM: Elsevier, 2019, roč. 205, DEC 1 2019, s. 1-8. ISSN 0039-9140. Dostupné z: https://dx.doi.org/10.1016/j.talanta.2019.07.006.

@article{1582256,
   author = {Jimenez, Ana Maria Jimenez and Moulick, Amitava and Bhowmick, Sukanya and Strmiska, Vladislav and Gagic, Milica and Horakova, Zuzana and Kostrica, Rom and Masařík, Michal and Heger, Zbynek and Adam, Vojtech},
   article_location = {AMSTERDAM},
   article_number = {DEC 1 2019},
   doi = {http://dx.doi.org/10.1016/j.talanta.2019.07.006},
   keywords = {Cancer; Human papillomavirus; Nanotechnology; Magnetic isolation; Quantum dots},
   language = {eng},
   issn = {0039-9140},
   journal = {Talanta},
   title = {One-step detection of human papilloma viral infection using quantum dot-nucleotide interaction specificity},
   url = {http://dx.doi.org/10.1016/j.talanta.2019.07.006},
   volume = {205},
   year = {2019}
}

TY  - JOUR
ID  - 1582256
AU  - Jimenez, Ana Maria Jimenez - Moulick, Amitava - Bhowmick, Sukanya - Strmiska, Vladislav - Gagic, Milica - Horakova, Zuzana - Kostrica, Rom - Masařík, Michal - Heger, Zbynek - Adam, Vojtech
PY  - 2019
TI  - One-step detection of human papilloma viral infection using quantum dot-nucleotide interaction specificity
JF  - Talanta
VL  - 205
IS  - DEC 1 2019
SP  - 1-8
EP  - 1-8
PB  - Elsevier
SN  - 00399140
KW  - Cancer
KW  - Human papillomavirus
KW  - Nanotechnology
KW  - Magnetic isolation
KW  - Quantum dots
UR  - http://dx.doi.org/10.1016/j.talanta.2019.07.006
L2  - http://dx.doi.org/10.1016/j.talanta.2019.07.006
N2  - Due to the close relationship between carcinogenesis and human papillomavirus (HPV), and since they are transmitted via huge number of asymptomatic carriers, the detection of HPV is really needed to reduce the risk of developing cancer. According to the best of our knowledge, our study provides the very first method for one-step detection of viral infection and if it has initiated the subsequent cancer proliferation. The proposed novel nanosystem consists of magnetic glass particles (MGPs), which were attached with DNA probe on their surface to hybridize with target DNAs. The MGP-probe-DNA hybrid was finally conjugated with CdTe/ZnSe core/shell quantum dots (QDs). The proposed detection system is based on a novel mechanism in which the MGPs separate out the target DNAs from different biological samples using external magnetic field for better and clear detection and the QDs give different fluorescent maxima for different target DNAs due to their ability to interact differently with different nucleotides. Firstly, the method was optimized using HPV genes cloned into synthetic plasmids. Then it was applied directly on the samples from normal and cancerous cells. After that, the real hospital samples of head and neck squamous cell carcinoma (HNSCC) with or without the infection of HPV were also analyzed. Our novel nano-system is proved successful in detecting and distinguishing between the patients suffering by HPV infection with or without subsequent cancer having detection limit estimated as 1.0 x 10(9) (GEq/mL). The proposed methodology is faster and cost-effective, which can be applied at the clinical level to help the doctors to decide the strategy of medication that may save the life of the patients with an early treatment.
ER  -

JIMENEZ, Ana Maria Jimenez, Amitava MOULICK, Sukanya BHOWMICK, Vladislav STRMISKA, Milica GAGIC, Zuzana HORAKOVA, Rom KOSTRICA, Michal MASAŘÍK, Zbynek HEGER a Vojtech ADAM. One-step detection of human papilloma viral infection using quantum dot-nucleotide interaction specificity. \textit{Talanta}. AMSTERDAM: Elsevier, 2019, roč.~205, DEC 1 2019, s.~1-8. ISSN~0039-9140. Dostupné z: https://dx.doi.org/10.1016/j.talanta.2019.07.006.

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