Prognostic Significance of Serum Free Amino Acids in Head and
Neck Cancers

J 2019

Prognostic Significance of Serum Free Amino Acids in Head and Neck Cancers

VŠIANSKÝ, Vít, Markéta SVOBODOVÁ, Jaromír GUMULEC, Natalia CERNEI, Dagmar STERBOVA et. al.

Základní údaje

Originální název

Prognostic Significance of Serum Free Amino Acids in Head and Neck Cancers

Autoři

VŠIANSKÝ, Vít (203 Česká republika, domácí), Markéta SVOBODOVÁ (203 Česká republika, domácí), Jaromír GUMULEC (203 Česká republika, domácí), Natalia CERNEI (203 Česká republika), Dagmar STERBOVA (203 Česká republika), Ondrej ZITKA (203 Česká republika), Rom KOSTRICA (203 Česká republika), Pavel SMILEK (203 Česká republika), Jan PLZAK (203 Česká republika), Jan BETKA (203 Česká republika), David KALFERT (203 Česká republika), Michal MASAŘÍK (203 Česká republika, domácí) a Martina RAUDENSKÁ (203 Česká republika, garant, domácí)

Vydání

Cells, Basel, MDPI, 2019, 2073-4409

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.366

Kód RIV

RIV/00216224:14110/19:00107806

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.3390/cells8050428

UT WoS

000470994400048

Klíčová slova anglicky

head and neck cancer; blood biomarkers; prognosis; colony forming assay; metabolism; amino acids

Štítky

14110515, 14110518, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 22. 11. 2019 10:05, Mgr. Tereza Miškechová

Anotace

V originále

Despite distinctive advances in the field of head and neck squamous cell cancer (HNSCC) biomarker discovery, the spectrum of clinically useful prognostic serum biomarkers is limited. As metabolic activities in highly proliferative transformed cells are fundamentally different from those in non-transformed cells, specific shifts in concentration of different metabolites may serve as diagnostic or prognostic markers. Blood amino acids have been identified as promising biomarkers in different cancers before, but little is known about this field in HNSCC. Blood amino acid profiles of 140 HNSCC patients were examined using high-performance liquid chromatography. Cox proportional hazards regression model was used to assess the prognostic value of amino acid concentrations in serum. Colony forming assay was used to identify the effect of amino acids that were significant in Cox proportional hazards regression models on colony forming ability of FaDu and Detroit 562 cell lines. In the multivariable Cox regression model for overall survival (OS), palliative treatment was associated with an unfavourable prognosis while high serum levels of methionine have had a positive prognostic impact. In the relapse-free survival (RFS) multivariable model, methionine was similarly identified as a positive prognostic factor, along with tumor localization in the oropharynx. Oral cavity localization and primary radio(chemo)therapy treatment strategy have been linked to poorer RFS. 1mM serine was shown to support the forming of colonies in both tested HNSCC cell lines. Effect of methionine was exactly the opposite.

Návaznosti

GA18-03978S, projekt VaV

Název: Efekt buněčné tuhosti a mikroprostředí jako prediktor odpovědi na léčbu karcinomů hlavy a krku

Investor: Grantová agentura ČR, Efekt buněčné tuhosti a mikroprostředí jako prediktor odpovědi na léčbu karcinomů hlavy a krku

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

NV18-08-00229, projekt VaV

Název: Interakce buněk nádorového mikroprostředí jako nový nástroj predikce účinnosti terapie nádorů hlavy a krku

Investor: Ministerstvo zdravotnictví ČR, Cell interactions in tumour microenvironment as a new tool for therapy response prediction in head and neck cancer

ROZV/24/LF/2018, interní kód MU

Název: LF - Příspěvek na IP 2108

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, LF - Příspěvek na IP 2108, Interní rozvojové projekty

Citovat

VŠIANSKÝ, Vít, Markéta SVOBODOVÁ, Jaromír GUMULEC, Natalia CERNEI, Dagmar STERBOVA, Ondrej ZITKA, Rom KOSTRICA, Pavel SMILEK, Jan PLZAK, Jan BETKA, David KALFERT, Michal MASAŘÍK a Martina RAUDENSKÁ. Prognostic Significance of Serum Free Amino Acids in Head and Neck Cancers. Cells. Basel: MDPI, 2019, roč. 8, č. 5, s. 1-12. ISSN 2073-4409. Dostupné z: https://dx.doi.org/10.3390/cells8050428.

@article{1582324,
   author = {Všianský, Vít and Svobodová, Markéta and Gumulec, Jaromír and Cernei, Natalia and Sterbova, Dagmar and Zitka, Ondrej and Kostrica, Rom and Smilek, Pavel and Plzak, Jan and Betka, Jan and Kalfert, David and Masařík, Michal and Raudenská, Martina},
   article_location = {Basel},
   article_number = {5},
   doi = {http://dx.doi.org/10.3390/cells8050428},
   keywords = {head and neck cancer; blood biomarkers; prognosis; colony forming assay; metabolism; amino acids},
   language = {eng},
   issn = {2073-4409},
   journal = {Cells},
   title = {Prognostic Significance of Serum Free Amino Acids in Head and Neck Cancers},
   url = {http://dx.doi.org/10.3390/cells8050428},
   volume = {8},
   year = {2019}
}

TY  - JOUR
ID  - 1582324
AU  - Všianský, Vít - Svobodová, Markéta - Gumulec, Jaromír - Cernei, Natalia - Sterbova, Dagmar - Zitka, Ondrej - Kostrica, Rom - Smilek, Pavel - Plzak, Jan - Betka, Jan - Kalfert, David - Masařík, Michal - Raudenská, Martina
PY  - 2019
TI  - Prognostic Significance of Serum Free Amino Acids in Head and Neck Cancers
JF  - Cells
VL  - 8
IS  - 5
SP  - 1-12
EP  - 1-12
PB  - MDPI
SN  - 20734409
KW  - head and neck cancer
KW  - blood biomarkers
KW  - prognosis
KW  - colony forming assay
KW  - metabolism
KW  - amino acids
UR  - http://dx.doi.org/10.3390/cells8050428
L2  - http://dx.doi.org/10.3390/cells8050428
N2  - Despite distinctive advances in the field of head and neck squamous cell cancer (HNSCC) biomarker discovery, the spectrum of clinically useful prognostic serum biomarkers is limited. As metabolic activities in highly proliferative transformed cells are fundamentally different from those in non-transformed cells, specific shifts in concentration of different metabolites may serve as diagnostic or prognostic markers. Blood amino acids have been identified as promising biomarkers in different cancers before, but little is known about this field in HNSCC. Blood amino acid profiles of 140 HNSCC patients were examined using high-performance liquid chromatography. Cox proportional hazards regression model was used to assess the prognostic value of amino acid concentrations in serum. Colony forming assay was used to identify the effect of amino acids that were significant in Cox proportional hazards regression models on colony forming ability of FaDu and Detroit 562 cell lines. In the multivariable Cox regression model for overall survival (OS), palliative treatment was associated with an unfavourable prognosis while high serum levels of methionine have had a positive prognostic impact. In the relapse-free survival (RFS) multivariable model, methionine was similarly identified as a positive prognostic factor, along with tumor localization in the oropharynx. Oral cavity localization and primary radio(chemo)therapy treatment strategy have been linked to poorer RFS. 1mM serine was shown to support the forming of colonies in both tested HNSCC cell lines. Effect of methionine was exactly the opposite.
ER  -

VŠIANSKÝ, Vít, Markéta SVOBODOVÁ, Jaromír GUMULEC, Natalia CERNEI, Dagmar STERBOVA, Ondrej ZITKA, Rom KOSTRICA, Pavel SMILEK, Jan PLZAK, Jan BETKA, David KALFERT, Michal MASAŘÍK a Martina RAUDENSKÁ. Prognostic Significance of Serum Free Amino Acids in Head and Neck Cancers. \textit{Cells}. Basel: MDPI, 2019, roč.~8, č.~5, s.~1-12. ISSN~2073-4409. Dostupné z: https://dx.doi.org/10.3390/cells8050428.

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