The interspecific variability of ladybird immunity and its response to stress conditions

FLORIÁN, Vojtěch, Michal ŘEŘICHA, Pavel DOBEŠ a Michal KNAPP. The interspecific variability of ladybird immunity and its response to stress conditions. In Kostelecké inspirování. 2019. ISBN 978-80-213-2981-2.

Základní údaje

• Originální název: The interspecific variability of ladybird immunity and its response to stress conditions
• Autoři: FLORIÁN, Vojtěch (203 Česká republika, garant, domácí), Michal ŘEŘICHA (203 Česká republika), Pavel DOBEŠ (203 Česká republika) a Michal KNAPP (203 Česká republika).
• Vydání: Kostelecké inspirování, 2019.

Další údaje

• Originální jazyk: angličtina
• Typ výsledku: Konferenční abstrakt
• Obor: 10616 Entomology
• Stát vydavatele: Česká republika
• Utajení: není předmětem státního či obchodního tajemství
• Kód RIV: RIV/00216224:14310/19:00111555
• Organizační jednotka: Přírodovědecká fakulta
• ISBN: 978-80-213-2981-2
• Klíčová slova anglicky: innate immunity; ladybird; antimicrobial activity; haemocyte concentration; total protein concentration; Harmonia axyridis; harlequin ladybird; antimicrobial peptides
• Příznaky: Mezinárodní význam

Anotace

Ladybirds (Coccinellidae) are the family of more than 6,000 species of beetles living worldwide. They differ in size, colour, territory, but also by type of nutriment. The best known are species that prey on aphids and scale insects. But some coccinellid species prey also on other arthropods or consume plant food. We compared immune reactions of 24 selected species of this large family, especially European species. The determined concentration of circulating haemocytes, antimicrobial activity against Gram-negative (Escherichia coli) and Gram-positive (Micrococcus luteus) bacteria were compared with fundamental physiological parameter, the total concentration of proteins in haemolymph. The comparison of basic immune parameters focused mainly on differences between species varying in ecology and invasiveness. Since harlequin ladybird (Harmonia axyridis) is an important invasive species, we further test its response to overwintering by measuring above mentioned immune parameters in individuals which were bred in different overwintering conditions. We have found that species from genera Harmonia, Ceratomegilla and Hippodamia have strong antimicrobial activity. Some other species, like seven-spot ladybird (Coccinella septempunctata) or two-spot ladybird (Adalia bipunctata), showed intermediate level of activity, but in many species we did not detected any antimicrobial activity against tested bacteria. Interestingly, three genera of ladybirds with strong immune activity had also the lowest concentration of proteins (50-80 mg/ml), whereas most of other species had concentration among 100-250 mg/ml.

Anotace česky

Ladybirds (Coccinellidae) are the family of more than 6,000 species of beetles living worldwide. They differ in size, colour, territory, but also by type of nutriment. The best known are species that prey on aphids and scale insects. But some coccinellid species prey also on other arthropods or consume plant food. We compared immune reactions of 24 selected species of this large family, especially European species. The determined concentration of circulating haemocytes, antimicrobial activity against Gram-negative (Escherichia coli) and Gram-positive (Micrococcus luteus) bacteria were compared with fundamental physiological parameter, the total concentration of proteins in haemolymph. The comparison of basic immune parameters focused mainly on differences between species varying in ecology and invasiveness. Since harlequin ladybird (Harmonia axyridis) is an important invasive species, we further test its response to overwintering by measuring above mentioned immune parameters in individuals which were bred in different overwintering conditions. We have found that species from genera Harmonia, Ceratomegilla and Hippodamia have strong antimicrobial activity. Some other species, like seven-spot ladybird (Coccinella septempunctata) or two-spot ladybird (Adalia bipunctata), showed intermediate level of activity, but in many species we did not detected any antimicrobial activity against tested bacteria. Interestingly, three genera of ladybirds with strong immune activity had also the lowest concentration of proteins (50-80 mg/ml), whereas most of other species had concentration among 100-250 mg/ml.