Fast blood plasma separation device for point-of-care
applications

J 2018

Fast blood plasma separation device for point-of-care applications

ĎURČ, Pavol; František FORET and Petr KUBÁŇ

Basic information

Original name

Fast blood plasma separation device for point-of-care applications

Authors

ĎURČ, Pavol (703 Slovakia, belonging to the institution); František FORET (203 Czech Republic) and Petr KUBÁŇ (203 Czech Republic, guarantor)

Edition

Talanta, Amsterdam, ELSEVIER SCIENCE BV, 2018, 0039-9140

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

10406 Analytical chemistry

Country of publisher

Netherlands

Confidentiality degree

is not subject to a state or trade secret

References:

Full Text

Impact factor

Impact factor: 4.916

RIV identification code

RIV/00216224:14310/18:00111646

Organization unit

Faculty of Science

DOI

https://doi.org/10.1016/j.talanta.2018.02.004

UT WoS

000430645800008

EID Scopus

2-s2.0-85042222134

Keywords in English

Blood plasma separation; Point of care analysis; Capillary electrophoresis; Alcohol intoxication; Metabolites; Methanol

Abstract

In the original language

In this work, a simple device for extremely fast separation of blood plasma from diluted whole blood was developed. The device accommodates an asymmetric polysulfone membrane/supporting membrane sandwich that allows collection of 10 mu L blood plasma into a narrow glass capillary in less than 10 s. The composition of diluent solution was optimized in order to achieve maximum recoveries for selected metabolites of alcohol intoxication. 5% solution of [tris(hydroxymethyl)methylamino] propanesulfonic acid provided recoveries of formate, oxalate and glycolate close to 100% and only moderate erythrocyte lysis. Both charged and uncharged compounds from the whole blood samples can be analyzed in the separated blood plasma by capillary electrophoresis with contactless conductometric detection and spectrophotometry, respectively. The developed device might find wide application in on-site testing and point-of-care analysis, when only microliter volumes of whole blood are available.

Cite

ĎURČ, Pavol; František FORET and Petr KUBÁŇ. Fast blood plasma separation device for point-of-care applications. Talanta. Amsterdam: ELSEVIER SCIENCE BV, 2018, vol. 183, JUN, p. 55-60. ISSN 0039-9140. Available from: https://doi.org/10.1016/j.talanta.2018.02.004.

@article{1590677,
   author = {Ďurč, Pavol and Foret, František and Kubáň, Petr},
   article_location = {Amsterdam},
   article_number = {JUN},
   doi = {https://doi.org/10.1016/j.talanta.2018.02.004},
   keywords = {Blood plasma separation; Point of care analysis; Capillary electrophoresis; Alcohol intoxication; Metabolites; Methanol},
   language = {eng},
   issn = {0039-9140},
   journal = {Talanta},
   title = {Fast blood plasma separation device for point-of-care applications},
   url = {https://www.sciencedirect.com/science/article/pii/S0039914018301127},
   volume = {183},
   year = {2018}
}

TY  - JOUR
ID  - 1590677
AU  - Ďurč, Pavol - Foret, František - Kubáň, Petr
PY  - 2018
TI  - Fast blood plasma separation device for point-of-care applications
JF  - Talanta
VL  - 183
IS  - JUN
SP  - 55-60
EP  - 55-60
PB  - ELSEVIER SCIENCE BV
SN  - 00399140
KW  - Blood plasma separation
KW  - Point of care analysis
KW  - Capillary electrophoresis
KW  - Alcohol intoxication
KW  - Metabolites
KW  - Methanol
UR  - https://www.sciencedirect.com/science/article/pii/S0039914018301127
L2  - https://www.sciencedirect.com/science/article/pii/S0039914018301127
N2  - In this work, a simple device for extremely fast separation of blood plasma from diluted whole blood was developed. The device accommodates an asymmetric polysulfone membrane/supporting membrane sandwich that allows collection of 10 mu L blood plasma into a narrow glass capillary in less than 10 s. The composition of diluent solution was optimized in order to achieve maximum recoveries for selected metabolites of alcohol intoxication. 5% solution of [tris(hydroxymethyl)methylamino] propanesulfonic acid provided recoveries of formate, oxalate and glycolate close to 100% and only moderate erythrocyte lysis. Both charged and uncharged compounds from the whole blood samples can be analyzed in the separated blood plasma by capillary electrophoresis with contactless conductometric detection and spectrophotometry, respectively. The developed device might find wide application in on-site testing and point-of-care analysis, when only microliter volumes of whole blood are available.
ER  -

ĎURČ, Pavol; František FORET and Petr KUBÁŇ. Fast blood plasma separation device for point-of-care applications. \textit{Talanta}. Amsterdam: ELSEVIER SCIENCE BV, 2018, vol.~183, JUN, p.~55-60. ISSN~0039-9140. Available from: https://doi.org/10.1016/j.talanta.2018.02.004.

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