Automated workflow for the study of drug-protein interactions based on capillary electrophoresis-frontal analysis combined with in-capillary mixing of interacting partners

GLATZ, Zdeněk, Lenka MICHALCOVÁ a Hana NEVÍDALOVÁ. Automated workflow for the study of drug-protein interactions based on capillary electrophoresis-frontal analysis combined with in-capillary mixing of interacting partners. In 30th International Symposium on Pharmaceutical and Biomedical Analysis (PBA 2019). 2019.

Základní údaje

Originální název

Automated workflow for the study of drug-protein interactions based on capillary electrophoresis-frontal analysis combined with in-capillary mixing of interacting partners

Autoři

GLATZ, Zdeněk, Lenka MICHALCOVÁ a Hana NEVÍDALOVÁ

Vydání

30th International Symposium on Pharmaceutical and Biomedical Analysis (PBA 2019), 2019

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Další údaje

Typ výsledku

Konferenční abstrakt

Utajení

není předmětem státního či obchodního tajemství

Příznaky

Mezinárodní význam, Recenzováno

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Anotace

V originále

In this study, the new CE-FA method was created. The in-line approach of the sample mixing directly inside the capillary is based on diffusion and it is generic, robust and rapid. The numerous injection parameters and incubation period were considered and optimized. The method was used to determine the binding parameters of model systems (propranolol/lidocaine BSA) and compared with the classic methods. Based on the statistical evaluation, it was found that the results achieved both approaches are almost identical. In-line sample mixing for the CE FA method has so far not been published and a combination of CE FA approaches gives rise to a fully automated and versatile method for characterizing binding interactions.

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Návaznosti

GA19-08358S, projekt VaV

Název: Nové přístupy pro studium afinitních interakcí založené na kapilární elektroforéze Investor: Grantová agentura ČR, Nove přístupy pro studium afinitních interakcí založené na kapilární elektroforéze